Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BKX93_RS09965 Genome accession   NZ_CP017707
Coordinates   2134025..2135731 (-) Length   568 a.a.
NCBI ID   WP_070979662.1    Uniprot ID   A0A1D9LG87
Organism   Chromobacterium vaccinii strain 21-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2129025..2140731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKX93_RS09940 (BKX93_09940) yacG 2130253..2130444 (-) 192 WP_046168374.1 DNA gyrase inhibitor YacG -
  BKX93_RS09945 (BKX93_09945) zapD 2130450..2131208 (-) 759 WP_031297029.1 cell division protein ZapD -
  BKX93_RS09950 (BKX93_09950) coaE 2131245..2131859 (-) 615 WP_046155583.1 dephospho-CoA kinase -
  BKX93_RS09955 (BKX93_09955) - 2131844..2132764 (-) 921 WP_046168368.1 A24 family peptidase -
  BKX93_RS09960 (BKX93_09960) pilC 2132764..2133987 (-) 1224 WP_046168367.1 type II secretion system F family protein Machinery gene
  BKX93_RS09965 (BKX93_09965) pilF 2134025..2135731 (-) 1707 WP_070979662.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BKX93_RS09970 (BKX93_09970) - 2135900..2137021 (-) 1122 WP_046155587.1 porin -
  BKX93_RS09975 (BKX93_09975) - 2137420..2137740 (-) 321 WP_070979663.1 helix-hairpin-helix domain-containing protein -
  BKX93_RS09980 (BKX93_09980) ccsA 2137807..2138628 (-) 822 WP_046155588.1 cytochrome c biogenesis protein CcsA -
  BKX93_RS09985 (BKX93_09985) ffh 2138740..2140095 (+) 1356 WP_046155589.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62359.98 Da        Isoelectric Point: 5.5625

>NTDB_id=202139 BKX93_RS09965 WP_070979662.1 2134025..2135731(-) (pilF) [Chromobacterium vaccinii strain 21-1]
MEATAGISGLGRALVQHNMLLQQDADAISKQAITSHLSFVEQLIQSKKMAARDIAMFSSRVFGYPLLDLASLDTDQLPKG
LVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFG
DLEFADPEAQLDNGPQTEVDDAPVVKFIHKMLLDAIGLGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLFGEKIVMRILDSSAASLDIEQLGFEPEQKAHLLRAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARKLCSHCKQPVEIPEEALLRAGFLK
EDLAGGWHPYGPVGCEECRNTGYRGRVGIFEVMPISDAMMRLIMKNGTAVDISDLAREEGMVDLRRAGLLKVIAGMTSLA
EVEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=202139 BKX93_RS09965 WP_070979662.1 2134025..2135731(-) (pilF) [Chromobacterium vaccinii strain 21-1]
ATGGAAGCTACGGCAGGGATTTCCGGCCTGGGCAGGGCCCTGGTCCAGCACAATATGCTGTTGCAGCAGGATGCGGACGC
CATTTCGAAACAGGCGATAACTTCCCATCTCAGCTTTGTCGAGCAGCTGATCCAGAGCAAGAAGATGGCCGCGCGCGACA
TCGCGATGTTTTCATCCAGAGTGTTCGGCTATCCGCTGCTGGATCTGGCTTCGCTGGATACCGACCAACTACCCAAGGGG
CTGGTGGACGACGAGGTGATCGCCAGCCGCCGGCTGCTGCCGCTGCTCAAGCGCGGCAACAAGCTTTACATCGGCACTTC
CGATCCCACCCAGACCTCCGCCTACCATGCGATCACTTTCAAGACCGGCCTGACGGTAGAAGTGGTCGTGGTGGAAGAAG
ACAAGCTGTCCAAGCTGATCGGGCGATATCAGGACAATTCGACTTCGGCGCTGAAAGAGCTGGAAAGCGAGGATTTCGGC
GATCTGGAATTCGCCGATCCCGAGGCGCAGCTGGACAACGGCCCGCAGACCGAGGTCGACGATGCTCCAGTGGTCAAGTT
CATCCACAAGATGTTGCTGGATGCGATAGGCCTCGGCGCGTCCGACATCCACTTCGAGCCATACGAGAAGTACTACCGCA
TCCGCTACCGGGTGGATGGCGTATTGAAGGAGGTGGCGCAGCCGCCCTTGCTGCTGAAGGAAAAGATCGCTTCCCGCATC
AAGGTGATTTCCCGGCTGGATATTTCGGAAAAGCGGCTGCCTCAGGATGGCCGGCTGAAGCTGGTGATTTCCAAGACCCG
GGCGATAGATTTCCGCGTCAGTACCTTGCCCACCTTGTTCGGCGAGAAAATCGTGATGCGTATCCTGGATTCGTCGGCGG
CCTCGCTGGACATCGAGCAGCTGGGCTTCGAGCCGGAGCAAAAAGCGCATCTGTTGAGGGCGATAGCCCGGCCCTACGGC
ATGGTCCTGGTGACCGGCCCTACCGGCAGCGGCAAGACCGTATCGCTTTACACTTGTCTGAACATTTTGAACAAGCCGGA
CACCAATATCTCCACTGCGGAGGATCCGGTGGAAATCAACCTGCCCGGCATCAATCAGGTCAACGTCAACGAAAAGGCGG
GGTTGACCTTCGCCTCGGCGTTGAAGGCTTTTCTGCGGCAAGATCCTGACGTGATCATGGTGGGCGAGATTCGCGATTTC
GAGACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGTTCTCGACGCTGCATACCAACGACGCGCCGTC
CACCTTGACGCGGATGCTGAACATGGGCGTGGCGCCGTTCAACATCGCCAGTTCGGTGTTGCTGATCATGGCGCAGCGCT
TGGCGCGCAAACTATGCAGCCATTGCAAGCAGCCGGTGGAGATTCCCGAGGAGGCGCTGCTGCGCGCAGGTTTCTTGAAG
GAGGACTTGGCCGGCGGCTGGCACCCCTACGGGCCGGTCGGCTGCGAGGAGTGCCGGAACACCGGCTACAGGGGGCGCGT
CGGGATTTTCGAAGTGATGCCGATCAGCGATGCGATGATGCGGCTGATCATGAAGAACGGCACTGCGGTGGACATCTCCG
ATCTGGCCAGGGAGGAGGGCATGGTCGATCTGCGCCGCGCCGGCCTGCTGAAGGTGATTGCCGGCATGACCTCGCTGGCC
GAGGTCGAGGCGGTGACCAACGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9LG87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

58.363

98.944

0.577
  pilB Acinetobacter baumannii D1279779

54.623

100

0.562
  pilB Legionella pneumophila strain ERS1305867

54.021

100

0.544
  pilB Acinetobacter baylyi ADP1

54.174

99.12

0.537
  pilB Vibrio cholerae strain A1552

49.113

99.296

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.313

99.12

0.479
  pilB Vibrio campbellii strain DS40M4

46.892

99.12

0.465
  pilF Thermus thermophilus HB27

38.612

98.944

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.299

94.366

0.38

Multiple sequence alignment