Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LT090_RS02835 Genome accession   NZ_CP017689
Coordinates   634304..636007 (-) Length   567 a.a.
NCBI ID   WP_415918826.1    Uniprot ID   -
Organism   Thalassotalea crassostreae strain LPB0090     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 629304..641007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT090_RS02810 yacG 629595..629831 (-) 237 WP_068544848.1 DNA gyrase inhibitor YacG -
  LT090_RS02815 - 629979..630725 (-) 747 WP_068544846.1 cell division protein ZapD -
  LT090_RS02820 coaE 631099..631707 (-) 609 WP_068545045.1 dephospho-CoA kinase -
  LT090_RS02825 pilD 631897..632790 (-) 894 WP_068544844.1 prepilin peptidase Machinery gene
  LT090_RS02830 pilC 632968..634221 (-) 1254 WP_082897042.1 type II secretion system F family protein Machinery gene
  LT090_RS02835 pilB 634304..636007 (-) 1704 WP_415918826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LT090_RS17240 - 636120..636509 (-) 390 WP_269466066.1 pilin -
  LT090_RS02845 nadC 637107..638000 (-) 894 WP_068544840.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LT090_RS02850 - 638043..638564 (-) 522 WP_415918824.1 TIGR02281 family clan AA aspartic protease -
  LT090_RS02855 ampD 638820..639392 (+) 573 WP_082897041.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LT090_RS02860 ampE 639607..640452 (+) 846 WP_068544838.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62344.37 Da        Isoelectric Point: 4.9734

>NTDB_id=201803 LT090_RS02835 WP_415918826.1 634304..636007(-) (pilB) [Thalassotalea crassostreae strain LPB0090]
MAMKGIHRQSSLLSAVVRSELIAKDKAADIFSFCQKKQTSIVSHLIEKEKISSKEITKVLSKSFGYPIIELKSLDLTALP
DGLRNEKLIQKHNALPLYLRGNILFIAVSDPTNIDALEEFQFNTGYSTEMVLADEVQLKSVIENLLEDESSALELTDLDE
DELGDIDVQDSEKKEEESNGENDAPIVVYINKILLDAIKKGASDLHFEPFEKKFRVRFRVDGILAEVASPPVNLASRMTA
RLKVMSKLDIAERRVPQDGRIKLNVSKTKSIDFRVSTLPTMWGEKVVMRILDSSSAQLGIDILGYEDEQKKLYMDALSKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVQINNRAGLTFAEALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSLIIAQRLARRLCNHCKTVDDVSPHALVELGF
EASEANDITIYKPVGCDECTKGYKGRVGIYEVMQISQETATIIMEGGSSLDIAKQAQSEGFNNLRQSALLKAKAGVTSLA
EVTRVTA

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=201803 LT090_RS02835 WP_415918826.1 634304..636007(-) (pilB) [Thalassotalea crassostreae strain LPB0090]
ATTGCTATGAAAGGAATTCATCGACAATCTAGTTTGCTCTCTGCTGTCGTGCGCAGTGAACTGATTGCAAAAGACAAAGC
CGCTGATATATTTTCTTTTTGTCAAAAAAAGCAAACGTCTATTGTCTCCCACTTAATTGAAAAAGAAAAAATCTCATCGA
AAGAGATTACTAAAGTACTATCGAAAAGCTTTGGTTATCCAATTATTGAACTGAAATCTCTAGATTTGACTGCATTGCCA
GACGGTCTTCGTAATGAAAAACTCATTCAAAAACACAATGCTCTGCCACTATATTTACGTGGCAATATCTTATTTATTGC
GGTATCCGATCCAACTAATATTGATGCCCTCGAAGAATTCCAATTTAATACCGGTTATAGCACAGAAATGGTGCTTGCTG
ACGAAGTTCAATTAAAATCCGTAATTGAAAATTTACTTGAAGATGAAAGTTCAGCACTCGAGCTCACCGACCTCGATGAA
GATGAGCTTGGCGATATCGATGTTCAAGATTCAGAAAAAAAAGAAGAAGAATCTAACGGTGAAAATGATGCACCGATTGT
TGTTTACATCAATAAAATACTACTCGATGCGATTAAAAAAGGCGCATCTGATTTGCATTTTGAGCCGTTCGAGAAAAAGT
TCCGCGTACGTTTCCGAGTTGATGGCATATTAGCCGAAGTCGCGTCTCCGCCAGTCAATTTGGCAAGCCGTATGACTGCC
AGATTAAAAGTTATGTCAAAACTCGATATTGCCGAGCGTCGAGTACCACAAGATGGTCGTATAAAACTTAATGTATCAAA
AACGAAATCCATCGATTTTCGTGTATCAACTCTTCCTACGATGTGGGGCGAAAAAGTAGTAATGCGTATACTTGACTCCT
CAAGTGCCCAGTTGGGGATTGATATCCTTGGTTACGAAGATGAGCAGAAAAAGCTTTATATGGACGCCTTGTCTAAACCA
CAAGGCATGATTCTGGTAACCGGCCCTACCGGCTCTGGTAAAACCGTATCACTGTATACTGGCCTAAACATTCTCAATAC
TGAAGAGCGCAATATTTCTACCGCCGAAGACCCAGTTGAGATTAACCTCGAAGGCGTTAACCAGGTACAAATTAATAACC
GCGCAGGATTAACTTTTGCTGAAGCGTTAAAGTCTTTTTTACGTCAAGATCCCGATATCGTTATGGTTGGTGAGATCCGA
GATTTGGAAACCGCTGAAATTGCCATAAAGGCCGCGCAAACTGGTCACTTGGTATTATCAACTCTGCATACCAACTCTGC
TGCTGAGACATTGACGCGATTATTAAACATGGGCGTACCATCGTATAACGTTGCAAGCTCGGTAAGTTTAATCATTGCCC
AACGTCTTGCCCGCCGTTTATGTAACCATTGTAAAACCGTCGATGACGTGTCTCCCCATGCATTGGTTGAATTGGGGTTT
GAGGCAAGTGAAGCCAATGATATTACAATCTATAAACCTGTGGGCTGTGATGAATGTACCAAAGGCTATAAAGGTCGTGT
TGGTATCTACGAAGTAATGCAAATTAGCCAAGAAACTGCCACTATTATTATGGAAGGTGGAAGTTCTTTAGATATAGCTA
AACAAGCGCAAAGTGAAGGTTTTAATAACTTACGTCAATCTGCGCTGTTAAAAGCTAAAGCTGGTGTAACAAGTTTAGCT
GAAGTAACCAGGGTTACAGCGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.396

94.533

0.552
  pilB Acinetobacter baylyi ADP1

57.728

94.709

0.547
  pilB Legionella pneumophila strain ERS1305867

53.217

100

0.54
  pilB Vibrio cholerae strain A1552

53.654

98.942

0.531
  pilB Vibrio parahaemolyticus RIMD 2210633

52.92

99.647

0.527
  pilB Vibrio campbellii strain DS40M4

52.22

99.295

0.519
  pilF Neisseria gonorrhoeae MS11

48.833

98.236

0.48

Multiple sequence alignment