Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BJK09_RS07385 Genome accession   NZ_CP017601
Coordinates   1625475..1627199 (+) Length   574 a.a.
NCBI ID   WP_027227022.1    Uniprot ID   A0A2S6EZP9
Organism   Legionella pneumophila strain D6026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1620475..1632199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJK09_RS07365 (BJK09_07260) - 1620768..1623296 (+) 2529 WP_027227019.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  BJK09_RS07370 (BJK09_07265) - 1623298..1623867 (-) 570 WP_027221706.1 hypoxanthine-guanine phosphoribosyltransferase -
  BJK09_RS07375 (BJK09_07270) - 1623910..1624236 (-) 327 WP_027227020.1 DUF1820 family protein -
  BJK09_RS07380 (BJK09_07275) - 1624419..1625162 (-) 744 WP_027227021.1 methyltransferase domain-containing protein -
  BJK09_RS07385 (BJK09_07280) pilB 1625475..1627199 (+) 1725 WP_027227022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJK09_RS07390 (BJK09_07285) pilC 1627205..1628425 (+) 1221 WP_027221710.1 type II secretion system F family protein Machinery gene
  BJK09_RS07395 (BJK09_07290) pilD 1628471..1629334 (+) 864 WP_027227023.1 prepilin peptidase Machinery gene
  BJK09_RS07400 (BJK09_07295) - 1629348..1630553 (-) 1206 WP_027227024.1 cyclic nucleotide-binding domain-containing protein -
  BJK09_RS07405 (BJK09_07300) - 1630663..1631256 (-) 594 WP_027227025.1 hypothetical protein -
  BJK09_RS07410 (BJK09_07305) - 1631272..1631727 (-) 456 WP_027227026.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62645.11 Da        Isoelectric Point: 5.4362

>NTDB_id=200634 BJK09_RS07385 WP_027227022.1 1625475..1627199(+) (pilB) [Legionella pneumophila strain D6026]
MALATDEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLVQYLVKNKILSAEQIALTAAQNFGVPMMDINCIEVDSI
PASLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSSLIDNLLTAKESQGLSDYVE
DSGDLEGLEISTEDEDQDVDTATSATDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLA
SRITARIKVMSNLDISERRIPQDGGFKMKISKARAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRSNFIK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCDQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=200634 BJK09_RS07385 WP_027227022.1 1625475..1627199(+) (pilB) [Legionella pneumophila strain D6026]
ATGGCTCTAGCTACAGATGAATATAGACTACAGGGAATTGGCCAGCTTCTAGTACTGGAAAAGTTGCTAGATAAAACCAA
AGCAATTGAGTTGCATAAATTGGCCGCAGCAGAAAAGATATCTTTAGTGCAATATCTTGTAAAAAATAAAATACTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCAATGATGGATATTAATTGCATTGAGGTGGACTCTATT
CCTGCGAGTCTGGTTAATGAGAAATTGATTAAACGTCATGCCATGGTCCCCCTGTTTAGTCGCGGCACTAACTTGTACCT
TGCAACAGATGATCCCAGTAAGCAAGCTTCATTAAAGGAAATTCAATTTCATACCGGACTGAATACTCATGCTATAGTTG
TAGAAACAGATAAACTGAGTTCTTTGATTGATAACTTATTAACAGCAAAGGAAAGTCAAGGCTTATCGGACTATGTCGAA
GACTCTGGTGATCTGGAAGGTTTGGAAATAAGTACCGAAGATGAAGATCAAGATGTTGATACAGCAACATCTGCAACAGA
TGATGCACCGATTGTAAAGTTCGTTAATAAAATTTTATTGGATGCGATAAGGCAAGGCGCTTCTGATATACATTTTGAGC
CTTATGAACGAGAATACCGGATCAGGTACAGGCAAGATGGTATTTTACATGAGGTAGCTACCCCACCGGCAAGCCTGGCA
TCCCGTATTACAGCGCGTATCAAGGTAATGTCTAATTTGGATATATCAGAGAGGCGTATACCCCAGGATGGCGGCTTTAA
AATGAAAATATCCAAAGCACGAGCGATTGATTTCAGAGTGAGCACCTGTCCAACCTCAGCCGGGGAAAAAGTGGTGATGC
GAGTACTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGAGGTCCAATTTCATTAAA
GCAATCCAGCGCCCACAGGGAATGATTTTAGTGACAGGCCCAACAGGTAGTGGAAAAACAGTTACCTTATATACGGCTTT
GAATATATTAAATACAATTGAAGTGAACATTTCGACTGCAGAGGATCCTGTTGAAATCAAGGTACCTGGCATCAATCAGG
TTAATATTAATCCTAAAGCCGGCCTAACCTTTTCCGGTGCATTGCGCTCGTTTCTAAGGCAAGACCCTGATATTATCATG
GTTGGTGAGATACGAGATCTTGAAACAGCTGAAATTGCTGTAAAAGCTGCGCAGACAGGGCATTTGGTTCTTTCTACACT
GCATACCAACAGTGCTGCTGAAACATTAAATCGTTTAGTGAACATGGGGATACCTACTTTTAACATTGCGAGTTCAGTTA
CATTAATTATTGCACAAAGGCTGGCAAGAAAATTATGTAATCAATGTAAGGCAGTGCGCGATGACTTTACCAATCAAGGT
TTGATTGAATTAGGCTTCAAGGAAGCTGATCTGGTTAATCTTAAATTATACAAAGCAGTAGGTTGTGATCAATGCACAAG
TGGCTATCGCGGGCGGGTTGGTCTGTTTGAGGTTTTACCAATGACGAAAGAGCTAGGCCAGTTGATTATGTCAGGTGGTA
ACTCCCTGGATATCTTAAAATTAGCCCAATCTGAAGGTATGCTGACTATCTTTCAATCGGGCATAGAAAAAGTCAAGGAA
GGGATCACAACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6EZP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

96.341

100

0.963
  pilB Acinetobacter baumannii D1279779

51.146

98.78

0.505
  pilB Acinetobacter baylyi ADP1

49.315

100

0.502
  pilB Vibrio cholerae strain A1552

49.723

94.251

0.469
  pilB Vibrio campbellii strain DS40M4

47.397

97.038

0.46
  pilF Neisseria gonorrhoeae MS11

46.466

98.606

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

48.513

93.728

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

92.16

0.368
  pilF Thermus thermophilus HB27

38.806

93.38

0.362

Multiple sequence alignment