Detailed information NTG0200636

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BJK09_RS07395
• Genome accession: NZ_CP017601
• Coordinates: 1628471..1629334 (+)
• Length: 287 a.a.
• NCBI ID: WP_027227023.1
• Uniprot ID: A0A2S6EZM3
• Organism: Legionella pneumophila strain D6026
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1623471..1634334

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BJK09_RS07375 (BJK09_07270)	-	1623910..1624236 (-)	327	WP_027227020.1	DUF1820 family protein	-
BJK09_RS07380 (BJK09_07275)	-	1624419..1625162 (-)	744	WP_027227021.1	methyltransferase domain-containing protein	-
BJK09_RS07385 (BJK09_07280)	pilB	1625475..1627199 (+)	1725	WP_027227022.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BJK09_RS07390 (BJK09_07285)	pilC	1627205..1628425 (+)	1221	WP_027221710.1	type II secretion system F family protein	Machinery gene
BJK09_RS07395 (BJK09_07290)	pilD	1628471..1629334 (+)	864	WP_027227023.1	prepilin peptidase	Machinery gene
BJK09_RS07400 (BJK09_07295)	-	1629348..1630553 (-)	1206	WP_027227024.1	cyclic nucleotide-binding domain-containing protein	-
BJK09_RS07405 (BJK09_07300)	-	1630663..1631256 (-)	594	WP_027227025.1	hypothetical protein	-
BJK09_RS07410 (BJK09_07305)	-	1631272..1631727 (-)	456	WP_027227026.1	hypothetical protein	-
BJK09_RS07415 (BJK09_07310)	-	1631875..1632690 (+)	816	WP_027227027.1	pyruvate, water dikinase regulatory protein	-
BJK09_RS07420 (BJK09_07315)	-	1632772..1634226 (-)	1455	WP_027227028.1	bifunctional 2-methylcitrate dehydratase/aconitate hydratase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32867.63 Da
• Isoelectric Point: 8.2759

>NTDB_id=200636 BJK09_RS07395 WP_027227023.1 1628471..1629334(+) (pilD) [Legionella pneumophila strain D6026]
MINTLIINYTWFMYLVIGLFSLAVGSLLNVIIYRLPIILQEEWREQCCELLNLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCKSPISIRYPLIELLTTVLSLYASWHFGFTVQLLFVLLAIWILIPLTFIDLDHQLLPDSLTL
GLLWLGLIANTWSIFVSLDIAVLSCAGAYLALWLFINLFYLITGKVGMGHGDFKLFAAFGAWFGWMYLPLILLMSSITGA
IIGLIYLRINGKSRDTAIPFGPFLCISGLIAMFLGDSIINWYIGYWM

Nucleotide

• Length: 864 bp

>NTDB_id=200636 BJK09_RS07395 WP_027227023.1 1628471..1629334(+) (pilD) [Legionella pneumophila strain D6026]
ATGATAAATACGCTAATAATCAATTATACCTGGTTTATGTATTTAGTCATCGGACTCTTTTCTTTGGCCGTTGGAAGTTT
ACTTAATGTTATCATCTATCGCTTACCAATTATTTTGCAGGAAGAATGGCGAGAACAATGCTGTGAGTTACTTAATCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATCTATTTTTACCACGTTCATTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCCCTATTAAGCTATATCTTGTTAAGAGGTCGCTGTTACCAATGCAAAAGTCCCATTTCAATTCGCTA
TCCCCTTATTGAGTTATTGACCACAGTGTTATCATTATACGCCAGTTGGCACTTTGGATTTACAGTACAATTATTATTTG
TTTTATTAGCCATCTGGATATTGATACCTCTTACTTTTATTGATCTTGATCATCAATTACTCCCTGATAGTCTCACTTTA
GGACTATTATGGCTTGGTCTTATTGCAAATACATGGAGTATATTTGTTAGTCTTGATATTGCTGTGTTAAGTTGTGCCGG
TGCCTACTTAGCCTTATGGTTATTCATTAATCTATTTTATCTGATTACAGGTAAGGTAGGCATGGGGCATGGTGATTTCA
AATTATTTGCAGCATTTGGCGCATGGTTTGGCTGGATGTATTTACCATTAATTCTTCTGATGTCTTCAATAACCGGAGCT
ATCATTGGTTTGATTTATTTAAGGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGTCCTTTTCTTTGCATTTC
CGGGTTAATAGCTATGTTTTTGGGGGACTCCATTATCAATTGGTATATAGGTTATTGGATGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: A0A2S6EZM3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.534	97.909	0.495
pilD	Acinetobacter nosocomialis M2	49.084	95.122	0.467
pilD	Acinetobacter baumannii D1279779	48.364	95.819	0.463
pilD	Vibrio campbellii strain DS40M4	46.403	96.864	0.449
pilD	Neisseria gonorrhoeae MS11	46.043	96.864	0.446