Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BG575_RS04960 Genome accession   NZ_CP017503
Coordinates   976706..978109 (+) Length   467 a.a.
NCBI ID   WP_006251341.1    Uniprot ID   A0A378NHR6
Organism   Mannheimia haemolytica strain 32795     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 971706..983109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG575_RS04925 (BG575_04825) - 971779..972543 (-) 765 WP_006248395.1 TSUP family transporter -
  BG575_RS04930 (BG575_04830) proB 972543..973634 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  BG575_RS04935 (BG575_04835) folA 973746..974240 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG575_RS04940 (BG575_04840) - 974360..974590 (+) 231 WP_006251345.1 YgjV family protein -
  BG575_RS04945 (BG575_04845) radA 974645..976024 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  BG575_RS04955 (BG575_04850) pilA 976206..976685 (+) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  BG575_RS04960 (BG575_04855) pilB 976706..978109 (+) 1404 WP_006251341.1 GspE/PulE family protein Machinery gene
  BG575_RS04965 (BG575_04860) pilC 978093..979289 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG575_RS04970 (BG575_04865) - 979292..979948 (+) 657 WP_006248404.1 prepilin peptidase -
  BG575_RS04975 (BG575_04870) coaE 979984..980619 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  BG575_RS04980 (BG575_04875) yacG 980601..980786 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  BG575_RS04985 (BG575_04880) holA 980787..981812 (-) 1026 WP_032844853.1 DNA polymerase III subunit delta -
  BG575_RS04990 (BG575_04885) lptE 981855..982343 (-) 489 WP_006251337.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 52965.62 Da        Isoelectric Point: 5.5059

>NTDB_id=199180 BG575_RS04960 WP_006251341.1 976706..978109(+) (pilB) [Mannheimia haemolytica strain 32795]
MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAEQLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEEL
KFLLNALSPEQSSPQSEIYEENPLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGEKVVLRLQKNKPTHFDFLDLGFN
PTQKANLLNALSQPQGLILVTGPTGSGKSITLYSALSHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTF
LRQDPDIIMLGEIRDEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLRRVCVKCGG
AGCEHCYQGYKGRIGVYQLLNRSAKNFEKNTACLDFKTLADSAKEKLTEGKTNQAEILRVLGNDENL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=199180 BG575_RS04960 WP_006251341.1 976706..978109(+) (pilB) [Mannheimia haemolytica strain 32795]
ATGGAGTATTCGGTAACCGACTTAAACAGCCAAAGATTCATCGAGATTTCAGCAGAACAATGGCAGAAAAATTGTAATGA
AAAGCAGATTTTGTTGCGTTACTTAGCCGTTCCACTGCAAGAAACAGCAGAACAGCTTTGGCTGGGAATTGATGATATGC
AAAATCTTAATGCCTGCGAGATTTTTGCATTTATCTACCACAAACAGATTGAGCCTGTTTTGATTTCCTCCGAAGAATTA
AAATTTTTGCTCAATGCCCTTTCGCCTGAACAGAGTAGCCCGCAGAGTGAAATTTATGAAGAAAATCCGCTTTATCCTTT
TTCCGAGAATACCGAGCAGCCGGATAGAAATGATCCGATCATCCAAATTTTAGAGAATTTGTTTAAGTTCTGTTTGCAAC
AGAACGCTTCCGATATCCATCTTGAACCACAAAAAGAGAGATTGTTAATTCGGTTAAGAATTGATGGCGTGCTACATATT
TATAAAAGCCTGCCGAGTAGTTTATCCAGCCGTTTGATTTCACGGGTTAAATTATTGGCTAAGCTCGATATCAGCGAGAC
TCGTTTGCCACAAGACGGACAATTTCATTTCAAAACACTGTTAGCTGAAACGCTGGATTTCCGTGTTTCTACCTTGCCAA
CGCACTTCGGCGAAAAAGTGGTGCTACGGCTACAAAAAAATAAACCAACCCATTTTGATTTTTTAGATTTAGGCTTTAAC
CCAACTCAGAAAGCCAATTTGCTGAATGCTTTGAGTCAGCCTCAAGGATTGATTTTAGTAACCGGCCCGACAGGAAGCGG
TAAAAGCATCACGCTTTACAGTGCATTAAGCCATTTAAACAGCAGTGAAAAGCATATTTTGACTGCCGAAGACCCGATTG
AAATTGAGATTGAGGGAATTATCCAAACCCAAGTGAATCGAGGGATTAACTTAGATTTCAGCCAACTGTTAAGAACCTTT
CTACGCCAAGATCCGGACATTATTATGCTCGGCGAAATTCGTGATGAAGAGAGTGCAGAAATGGCACTACGGGCGGCACA
AACCGGGCATTTGGTGCTTTCGACTTTGCACACCAATGATGCCCCTTCAGCGGTAGAACGGTTGCTACAGCTCGGCATAA
AAGAGTATGAACTTAAAAATTCATTGCTACTCGTGATTGCTCAACGCCTGTTACGCCGAGTGTGTGTGAAATGCGGTGGG
GCAGGCTGTGAGCATTGTTATCAAGGGTATAAAGGGCGAATTGGGGTATATCAACTCTTAAACCGATCTGCAAAAAATTT
TGAAAAAAACACCGCTTGTTTGGATTTTAAAACCCTTGCCGACAGTGCAAAAGAAAAATTGACGGAAGGAAAAACCAATC
AGGCGGAAATTTTAAGGGTGCTAGGCAATGACGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(132-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378NHR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.451

98.715

0.715
  pilB Haemophilus influenzae Rd KW20

56.71

98.929

0.561
  pilB Haemophilus influenzae 86-028NP

55.94

99.143

0.555
  pilB Vibrio campbellii strain DS40M4

40.748

100

0.42
  pilB Vibrio cholerae strain A1552

40.249

100

0.415
  pilB Legionella pneumophila strain ERS1305867

39.754

100

0.415
  pilB Vibrio parahaemolyticus RIMD 2210633

38.755

100

0.413
  pilB Acinetobacter baylyi ADP1

35.928

100

0.385
  pilB Acinetobacter baumannii D1279779

36.401

100

0.381

Multiple sequence alignment