Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BG575_RS04955 Genome accession   NZ_CP017503
Coordinates   976206..976685 (+) Length   159 a.a.
NCBI ID   WP_020831302.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 32795     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 971206..981685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG575_RS04925 (BG575_04825) - 971779..972543 (-) 765 WP_006248395.1 TSUP family transporter -
  BG575_RS04930 (BG575_04830) proB 972543..973634 (-) 1092 WP_006251346.1 glutamate 5-kinase -
  BG575_RS04935 (BG575_04835) folA 973746..974240 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG575_RS04940 (BG575_04840) - 974360..974590 (+) 231 WP_006251345.1 YgjV family protein -
  BG575_RS04945 (BG575_04845) radA 974645..976024 (-) 1380 WP_006251344.1 DNA repair protein RadA -
  BG575_RS04955 (BG575_04850) pilA 976206..976685 (+) 480 WP_020831302.1 prepilin peptidase-dependent pilin Machinery gene
  BG575_RS04960 (BG575_04855) pilB 976706..978109 (+) 1404 WP_006251341.1 GspE/PulE family protein Machinery gene
  BG575_RS04965 (BG575_04860) pilC 978093..979289 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG575_RS04970 (BG575_04865) - 979292..979948 (+) 657 WP_006248404.1 prepilin peptidase -
  BG575_RS04975 (BG575_04870) coaE 979984..980619 (+) 636 WP_020831305.1 dephospho-CoA kinase -
  BG575_RS04980 (BG575_04875) yacG 980601..980786 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16855.17 Da        Isoelectric Point: 8.7964

>NTDB_id=199179 BG575_RS04955 WP_020831302.1 976206..976685(+) (pilA) [Mannheimia haemolytica strain 32795]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVELCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=199179 BG575_RS04955 WP_020831302.1 976206..976685(+) (pilA) [Mannheimia haemolytica strain 32795]
ATGCAAAAAATTGTACATTTTCGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTAATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACCATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTGCAAGCCTCTGCTTCGTATAAATCAGACGTTGAACTCTGTATTTACAACAGTGGCGGGCTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

57.143

88.05

0.503
  pilA Haemophilus influenzae Rd KW20

55.634

89.308

0.497

Multiple sequence alignment