Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BIZ42_RS05445 Genome accession   NZ_CP017483
Coordinates   1239389..1241119 (-) Length   576 a.a.
NCBI ID   WP_019183939.1    Uniprot ID   -
Organism   Stenotrophomonas sp. LM091     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1234389..1246119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIZ42_RS05430 (BIZ42_05430) sucC 1234724..1235893 (-) 1170 WP_070425790.1 ADP-forming succinate--CoA ligase subunit beta -
  BIZ42_RS05435 (BIZ42_05435) - 1236227..1237840 (+) 1614 WP_070425791.1 ATP-binding protein -
  BIZ42_RS05440 (BIZ42_05440) pilR 1237911..1239296 (+) 1386 WP_046273839.1 sigma-54 dependent transcriptional regulator Regulator
  BIZ42_RS05445 (BIZ42_05445) pilB 1239389..1241119 (-) 1731 WP_019183939.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BIZ42_RS19975 (BIZ42_05450) - 1241256..1241633 (-) 378 WP_070425792.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BIZ42_RS05455 (BIZ42_05455) pilC 1241995..1243254 (+) 1260 WP_070427451.1 type II secretion system F family protein Machinery gene
  BIZ42_RS05460 (BIZ42_05460) pilD 1243262..1244125 (+) 864 WP_046273837.1 A24 family peptidase Machinery gene
  BIZ42_RS05465 (BIZ42_05465) coaE 1244136..1244747 (+) 612 WP_070425793.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62215.32 Da        Isoelectric Point: 6.2060

>NTDB_id=198707 BIZ42_RS05445 WP_019183939.1 1239389..1241119(-) (pilB) [Stenotrophomonas sp. LM091]
MNTVAATANLVGITGIARRLVQDGALDEAAARTAMADATAARQPLPQWFAEKKLVTAAQLAAANALEFGMPLLDVSAFDS
SHNAMKLVSEELLRKHHVLPLFKRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILVDEEQIRRTLEQWQTSQDTLGSALGG
GDDDMGDLDVSAGDEEGGTTDAGIDAKGDDTPVVKFINKVLIDAIRKGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPAQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRPVELPAN
ALLAEGFTQAQLDAGIKLFEPVGCDECTEGYKGRTGIYQVMPMTDEISTIVLAGGNALQIAEAAQQAGVNDLRQSALAKA
AAGVTGLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=198707 BIZ42_RS05445 WP_019183939.1 1239389..1241119(-) (pilB) [Stenotrophomonas sp. LM091]
ATGAACACTGTCGCCGCCACCGCCAACCTCGTCGGCATCACCGGCATCGCCCGCCGCCTGGTCCAGGACGGCGCGCTGGA
CGAGGCCGCCGCCCGCACCGCCATGGCCGACGCCACCGCCGCCCGGCAGCCGCTGCCGCAGTGGTTCGCGGAGAAGAAGC
TCGTCACCGCCGCACAGCTGGCGGCCGCCAACGCGCTGGAGTTCGGCATGCCGCTGCTGGACGTGTCCGCGTTCGACAGC
AGCCACAACGCCATGAAGCTGGTGAGCGAGGAGTTGCTGCGCAAGCACCACGTGCTGCCACTGTTCAAGCGCGGTGGCAG
GCTGTTCGTGGGCACCAGCAACCCGACCCATTCGCTGGATGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCC
TGGTGGACGAGGAACAGATCCGCCGCACGCTGGAGCAGTGGCAGACCAGCCAGGACACCCTGGGCTCGGCGCTGGGCGGC
GGCGACGATGACATGGGCGACCTGGATGTCTCGGCCGGCGACGAGGAAGGCGGTACCACCGATGCGGGGATCGACGCCAA
GGGCGATGACACCCCGGTCGTCAAATTCATCAACAAGGTCCTGATCGACGCCATCCGAAAGGGCGCCTCGGACATCCATT
TCGAACCCTACGAAGACGACTATCGCGTGCGCCTGCGCATCGATGGTCTGCTCAAGAACGTGGCCAAGGCCCCGGTCAAG
CTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGATGGGCG
CATCAAGCTCAACCTCTCCAAGACCCGCCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTCGATGGCAGCGCTGCCAAACTGGGCATCGACAAGCTCGGGTATGAGCCGGCGCAGCAGAAACTGTTC
CTGGATGCGATTCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTGGGCATCCTCAACGATGAGACGCGCAACATTTCCACCGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGATCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCACATGGTGCTCTC
CACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCGTACAACATCACCAGCT
CGGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGCAACCACTGCAAGCGCCCGGTGGAGCTGCCGGCCAAC
GCGCTGCTGGCCGAGGGCTTCACCCAGGCCCAGCTGGACGCGGGCATCAAGCTGTTCGAGCCGGTCGGCTGCGATGAGTG
CACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCTCCACGATCGTGCTGGCCG
GTGGCAATGCGCTGCAGATCGCCGAGGCCGCGCAGCAGGCCGGGGTGAACGATCTGCGCCAGTCCGCGTTGGCCAAGGCC
GCCGCCGGGGTGACCGGTCTGGCGGAGATCAACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.732

98.438

0.549
  pilB Acinetobacter baylyi ADP1

55.556

98.438

0.547
  pilB Legionella pneumophila strain ERS1305867

52.705

99.479

0.524
  pilF Neisseria gonorrhoeae MS11

48.677

98.438

0.479
  pilB Vibrio cholerae strain A1552

48.336

99.132

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

45.694

98.785

0.451
  pilB Vibrio campbellii strain DS40M4

44.93

99.306

0.446

Multiple sequence alignment