Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BJI69_RS13035 Genome accession   NZ_CP017480
Coordinates   2867815..2869542 (-) Length   575 a.a.
NCBI ID   WP_046966391.1    Uniprot ID   A0A0G9HEM5
Organism   Luteibacter rhizovicinus DSM 16549 strain LJ96     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2862815..2874542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJI69_RS13015 (BJI69_12950) - 2863488..2864741 (+) 1254 WP_052767041.1 GGDEF domain-containing phosphodiesterase -
  BJI69_RS13020 (BJI69_12955) coaE 2864852..2865457 (-) 606 WP_046966388.1 dephospho-CoA kinase -
  BJI69_RS13025 (BJI69_12960) - 2865454..2866323 (-) 870 WP_046966389.1 A24 family peptidase -
  BJI69_RS13030 (BJI69_12965) pilC 2866405..2867670 (-) 1266 WP_046966390.1 type II secretion system F family protein Machinery gene
  BJI69_RS13035 (BJI69_12970) pilB 2867815..2869542 (-) 1728 WP_046966391.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJI69_RS13040 (BJI69_12975) - 2869714..2870568 (+) 855 WP_046966392.1 glycosyltransferase family 2 protein -
  BJI69_RS13045 (BJI69_12980) - 2870574..2871551 (+) 978 WP_052767042.1 glycosyltransferase -
  BJI69_RS13050 (BJI69_12985) - 2871555..2872334 (+) 780 WP_046966393.1 class I SAM-dependent methyltransferase -
  BJI69_RS13055 (BJI69_12990) - 2872331..2873353 (-) 1023 WP_046966394.1 glycosyltransferase -
  BJI69_RS13060 (BJI69_12995) - 2873350..2874303 (-) 954 WP_078023177.1 NAD-dependent epimerase/dehydratase family protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62790.46 Da        Isoelectric Point: 6.2683

>NTDB_id=198653 BJI69_RS13035 WP_046966391.1 2867815..2869542(-) (pilB) [Luteibacter rhizovicinus DSM 16549 strain LJ96]
MASQMQSPMLAGLTGMARRLVTEGVLPEADVRKAVQDSAEKRVSLSAWLVDHNLVDSPRLSQVASSEFGMPLMDIGNMAT
ANMPLDLVTEALISKHQALPLFKRGKRLFVGIADPMQSHALDEIKFHSNHMVEPVLVERGQLRRIIDAALASMGSQVAGY
EDGALDELSLEGGDGDDEPTSGIDATAGDDAPIVKFVNKILVDAIKRGASDIHFEPFETVYRVRLRMDGILRIVATAPIK
LGNRFASRLKVMSGLDIAERRVPQDGRIKLNLTKTRAIDFRVSTLPTLFGEKIVLRILDGSSAKLGIDKLGYEEVQKNLY
LDAIEKPYGMVLVTGPTGSGKTVSLYTALNILNTEGRNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNIISSVTLIIAQRLARRLHDCKREIVLPATA
LLAEGYTQEEIDAGFKVYEAVGCDSCNEGYKGRVGIYQVMPLVEDIQKIILEGGNAMQIGEVARRNGINDLRASALLKVR
NGMTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=198653 BJI69_RS13035 WP_046966391.1 2867815..2869542(-) (pilB) [Luteibacter rhizovicinus DSM 16549 strain LJ96]
ATGGCTTCACAAATGCAGTCGCCCATGCTTGCGGGTCTGACGGGTATGGCACGTCGGTTAGTCACCGAGGGTGTCCTGCC
CGAGGCGGACGTACGCAAGGCAGTCCAGGACTCGGCCGAAAAACGGGTCTCGCTGTCTGCGTGGCTCGTCGATCACAACC
TGGTCGACAGCCCCCGGCTGTCCCAGGTGGCGTCGTCCGAGTTCGGCATGCCGCTGATGGACATCGGCAACATGGCCACG
GCCAACATGCCGCTGGACCTGGTGACCGAAGCCCTGATCAGCAAGCACCAGGCCCTACCCCTGTTCAAGCGCGGCAAGCG
CCTGTTCGTCGGCATTGCCGATCCGATGCAGTCGCATGCGCTGGACGAGATCAAGTTCCACTCGAACCACATGGTCGAGC
CGGTGCTGGTCGAACGCGGCCAGTTGCGTCGCATCATCGACGCCGCGTTGGCATCGATGGGCTCGCAGGTCGCCGGTTAC
GAAGACGGCGCGCTCGACGAGCTGTCCCTCGAAGGCGGCGACGGTGACGACGAGCCGACCTCCGGCATCGACGCGACGGC
GGGCGATGACGCGCCGATCGTGAAGTTCGTCAACAAGATCCTGGTCGACGCGATCAAGCGTGGCGCCTCGGACATCCACT
TCGAACCCTTCGAAACCGTCTACCGTGTGCGCCTGCGAATGGACGGCATCCTGCGCATTGTCGCGACGGCGCCGATCAAG
CTCGGCAATCGCTTCGCCTCGCGCTTGAAGGTGATGAGCGGCCTGGATATCGCCGAGCGCCGCGTGCCGCAGGATGGCCG
CATCAAGCTCAACCTGACCAAAACCCGCGCGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGTTCCTCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGGAGGTCCAGAAGAACCTCTAC
CTCGACGCCATCGAAAAACCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCCGGTAAGACGGTGTCGCTGTACAC
GGCCTTGAACATCCTCAATACCGAGGGGCGCAACATCTCCACGGTGGAAGACCCGGTCGAAATCCGCGTCGAGGGTATCA
ACCAGGTTCAACAGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCCCTGCGCTCCTTCCTCCGCCAGGATCCGGATGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAAATTGCGGTCAAGGCCGCGCAGACGGGCCACATGGTTCTGTC
CACCCTGCATACCAACGACGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGCATCGCGCCCTACAACATCATTTCGT
CGGTGACCCTGATCATCGCCCAGCGCCTGGCCCGCCGCCTGCATGACTGCAAGCGCGAGATCGTCCTGCCGGCGACGGCC
TTGCTGGCCGAGGGTTATACCCAGGAAGAAATAGACGCTGGGTTCAAGGTCTACGAGGCCGTGGGCTGCGACAGCTGCAA
CGAGGGCTACAAGGGCCGCGTCGGTATCTACCAGGTCATGCCCCTGGTCGAGGACATCCAGAAGATCATCCTCGAGGGCG
GCAACGCGATGCAGATTGGCGAGGTCGCCCGCCGGAATGGCATCAACGACCTGCGTGCGTCTGCCCTGCTCAAGGTCCGC
AATGGCATGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G9HEM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.044

98.261

0.541
  pilB Acinetobacter baylyi ADP1

53.71

98.435

0.529
  pilB Legionella pneumophila strain ERS1305867

50.439

98.957

0.499
  pilB Vibrio cholerae strain A1552

48.49

97.913

0.475
  pilB Vibrio campbellii strain DS40M4

46.892

97.913

0.459
  pilF Neisseria gonorrhoeae MS11

50.19

91.478

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

45.979

99.478

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.027

100

0.372
  pilF Thermus thermophilus HB27

37.857

97.391

0.369

Multiple sequence alignment