Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XCCCN12_RS17410 Genome accession   NZ_CP017310
Coordinates   3804297..3806003 (-) Length   568 a.a.
NCBI ID   WP_187305920.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris str. CN12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3799297..3811003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XCCCN12_RS17400 - 3800665..3802275 (+) 1611 WP_040940804.1 HAMP domain-containing sensor histidine kinase -
  XCCCN12_RS17405 pilR 3802485..3803879 (+) 1395 WP_162808570.1 sigma-54 dependent transcriptional regulator Regulator
  XCCCN12_RS17410 pilB 3804297..3806003 (-) 1707 WP_187305920.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XCCCN12_RS17415 - 3806062..3806472 (-) 411 WP_075285411.1 pilin -
  XCCCN12_RS17420 - 3806552..3807001 (-) 450 WP_075285410.1 pilin -
  XCCCN12_RS17425 pilC 3807338..3808594 (+) 1257 WP_075285409.1 type II secretion system F family protein Machinery gene
  XCCCN12_RS17430 - 3808600..3809463 (+) 864 WP_075285408.1 A24 family peptidase -
  XCCCN12_RS17435 coaE 3809477..3810100 (+) 624 WP_075285407.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61805.86 Da        Isoelectric Point: 5.7640

>NTDB_id=197563 XCCCN12_RS17410 WP_187305920.1 3804297..3806003(-) (pilB) [Xanthomonas campestris pv. campestris str. CN12]
MGITGIARRLVQDGSLEEGAARSAMDQAAAAKIPLPQWFAERKLVSAAQLAAANAVEFGMPLMDVSVFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMGDDDEGMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHALLAEGF
TPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMKASHGVTSLA
EINRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=197563 XCCCN12_RS17410 WP_187305920.1 3804297..3806003(-) (pilB) [Xanthomonas campestris pv. campestris str. CN12]
ATCGGAATAACTGGAATAGCCCGTCGTTTGGTACAGGACGGCTCATTGGAAGAAGGCGCTGCGCGTTCAGCAATGGACCA
GGCTGCTGCCGCAAAAATTCCACTGCCTCAGTGGTTTGCTGAAAGAAAATTAGTATCTGCAGCTCAGCTCGCCGCAGCTA
ATGCCGTAGAGTTCGGCATGCCGCTGATGGATGTGTCGGTGTTTGACGCCAGCCAGAACGCGGTCAAGTTGGTCAGTGAG
GAGTTGCTTCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTTGTGGGGGTGAGCAACCCAACCCA
GACCCGTGCGCTGGACGACATCAAGTTCCATACTAATCTTGTGGTCGAGCCGATCCTCGTCGACGAGGATCAGATCCGGC
GCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGTTCGTCGATGGGGGACGACGACGAGGGGATGGGAGACCTA
GACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGATGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTCAACCAGCGCATCGCGGCG
CGCTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAAGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTGAGTACCTTGCCGACCCTGTTCGGCGAGAAGGTGGTGCTGCGTATCCTGGACGCCA
GTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAGCTGTTCCTGGATGCGATCCACAAGCCC
TACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCTGTATACCGCGCTCGGGATTCTCAACGA
CGAGACGCGCAATATTTCCACTGCTGAGGATCCGGTCGAAATCCGCTTGCCCGGCGTCAATCAGGTGCAGCAGAACGTCA
AGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCGCAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGCTTGGCACGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCGGAACATGCGCTGCTGGCGGAAGGCTTT
ACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGAATGCACGGAAGGCTACAAGGGCCGCAC
CGGTATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGGAAGGCGGCAATGCGATGCAGATCGCCG
AGGCCGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAGGCTTCGCACGGCGTGACCAGCCTGGCC
GAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

100

0.562
  pilB Acinetobacter baylyi ADP1

55.458

100

0.555
  pilB Legionella pneumophila strain ERS1305867

52.641

100

0.526
  pilF Neisseria gonorrhoeae MS11

48.592

100

0.486
  pilB Vibrio cholerae strain A1552

49.548

97.359

0.482
  pilB Vibrio campbellii strain DS40M4

46.397

100

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

46.691

98.415

0.46

Multiple sequence alignment