Detailed information NTG0194568

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: BGI51_RS02750
• Genome accession: NZ_CP017024
• Coordinates: 588840..590534 (-)
• Length: 564 a.a.
• NCBI ID: WP_187980237.1
• Uniprot ID: -
• Organism: Pseudomonas oryzihabitans strain KNF2016
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	585932..604382	588840..590534	within	0

Gene organization within MGE regions

Location: 585932..604382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BGI51_RS02735 (BGI51_02705)	coaE	585932..586543 (-)	612	WP_187980235.1	dephospho-CoA kinase	-
BGI51_RS02740 (BGI51_02710)	-	586540..587411 (-)	872	Protein_541	A24 family peptidase	-
BGI51_RS02745 (BGI51_02715)	pilC	587411..588628 (-)	1218	WP_187980236.1	type II secretion system F family protein	Machinery gene
BGI51_RS02750 (BGI51_02720)	pilB	588840..590534 (-)	1695	WP_187980237.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BGI51_RS02755	-	590747..591211 (+)	465	WP_187980238.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
BGI51_RS02760 (BGI51_02725)	-	591280..592317 (-)	1038	WP_187980239.1	SDR family oxidoreductase	-
BGI51_RS02770 (BGI51_02735)	cyoA	593090..594013 (+)	924	WP_133861286.1	ubiquinol oxidase subunit II	-
BGI51_RS02775 (BGI51_02740)	cyoB	594017..596005 (+)	1989	WP_158896248.1	cytochrome o ubiquinol oxidase subunit I	-
BGI51_RS02780 (BGI51_02745)	-	596010..596624 (+)	615	WP_133861288.1	cytochrome o ubiquinol oxidase subunit III	-
BGI51_RS02785 (BGI51_02750)	cyoD	596624..596962 (+)	339	WP_133861289.1	cytochrome o ubiquinol oxidase subunit IV	-
BGI51_RS02790 (BGI51_02755)	cyoE	596974..597861 (+)	888	WP_252977194.1	heme o synthase	-
BGI51_RS02795	-	597931..601848 (-)	3918	WP_252977195.1	pilus assembly protein	-
BGI51_RS02800 (BGI51_02765)	-	601845..602423 (-)	579	WP_252977196.1	PilX N-terminal domain-containing pilus assembly protein	-
BGI51_RS02805 (BGI51_02770)	-	602456..603253 (-)	798	WP_187980242.1	PilW family protein	-
BGI51_RS02810 (BGI51_02775)	pilV	603255..603848 (-)	594	WP_187980243.1	type IV pilus modification protein PilV	-
BGI51_RS02815 (BGI51_02780)	-	603849..604382 (-)	534	WP_187980244.1	GspH/FimT family pseudopilin	-

Sequence

Protein

• Length: 564 a.a.
• Molecular weight: 62421.79 Da
• Isoelectric Point: 5.7815

>NTDB_id=194568 BGI51_RS02750 WP_187980237.1 588840..590534(-) (pilB) [Pseudomonas oryzihabitans strain KNF2016]
MNETVMLSGLAKQLVDAQLLVEAAAQQAVVKAKQAKVPLVTYLVQQRLVQSRPLAELAAQEFGVPFLDLAAIDKEQLPKD
IVNEKLMQQHHFLPLSKRGNKLFIALSDPTNQQAINDVSFGSKLAVEVVLVEDDKLHQAIERYLDTGTGGLELSDLDLDL
EAEGGERPQEAVAGSDADDAPVVKFVNKMLLDAIKKGSSDLHFEPYEKMYRVRFRTDGVLHEAARPPVQLVSRISARLKV
MSNMDISERRKPQDGRVKLKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEDVQKELYLQALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTEDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNLATSVNLIIAQRLARKLCNKCKRVHEVPREALLAEGFREEE
VGSFTLYQPIGCEDCNAGYRGRVGIYEVVKVTPALQQMIMEEGNSIEIAARMRKEGFNDLRRSGLVKAMQGITSLEEINR
VTKD

Nucleotide

• Length: 1695 bp

>NTDB_id=194568 BGI51_RS02750 WP_187980237.1 588840..590534(-) (pilB) [Pseudomonas oryzihabitans strain KNF2016]
ATGAACGAAACGGTAATGCTATCGGGATTGGCGAAGCAGCTGGTGGATGCGCAGCTGTTGGTAGAGGCGGCAGCCCAGCA
GGCAGTGGTCAAGGCCAAGCAGGCCAAGGTGCCGCTGGTAACTTATCTCGTTCAGCAGCGGCTGGTACAGAGTCGTCCCC
TAGCGGAACTGGCCGCTCAGGAGTTTGGCGTGCCCTTTCTCGATCTGGCGGCCATCGACAAGGAGCAGCTGCCTAAGGAT
ATTGTCAACGAAAAGCTGATGCAGCAGCATCACTTCCTGCCTCTGAGCAAAAGAGGCAACAAGCTCTTTATCGCACTCTC
TGATCCGACCAATCAGCAGGCCATCAATGATGTGTCCTTTGGCAGCAAACTGGCGGTCGAGGTGGTGCTGGTCGAAGATG
ACAAGCTGCACCAAGCCATCGAGCGATATCTAGATACGGGTACCGGTGGTTTGGAGCTGTCCGATCTTGATCTTGATCTA
GAGGCCGAAGGTGGGGAGCGGCCACAGGAAGCTGTTGCGGGTTCTGATGCCGACGATGCGCCAGTGGTGAAGTTCGTCAA
CAAGATGCTGCTCGATGCCATCAAGAAGGGTTCGTCCGACCTGCATTTCGAGCCCTACGAAAAGATGTACCGGGTGCGTT
TCCGCACCGACGGTGTCCTGCACGAGGCCGCTCGACCGCCAGTGCAGCTGGTATCGCGAATTTCGGCGCGGCTCAAGGTG
ATGTCCAATATGGACATCTCCGAGCGGCGCAAACCACAGGATGGCCGCGTCAAGCTCAAGGTCTCCAAGACCAAGTCCAT
CGACTTCCGCGTAAATACTTTGCCCACTCTTTGGGGCGAGAAGATCGTGATGCGGATCCTGGATTCCTCCAGCGCCCAGA
TGGGAATCGATGCCCTGGGCTATGAGGATGTGCAGAAGGAGCTCTATCTGCAGGCACTCAAGCAGCCGCAGGGAATGATC
CTGGTGACCGGGCCTACTGGTTCCGGCAAGACGGTCTCCCTTTATACCGGCCTCAATATCCTCAATACCGAAGACATCAA
TATTTCCACCGCTGAAGATCCGGTGGAGATCAATCTGGAAGGCATCAACCAGGTCAACGTTAACCCGCGTCAGGGACTCG
ACTTCTCCCAGGCGCTGCGGGCCTTCCTGCGTCAGGATCCTGACGTGATCATGGTGGGTGAGATTCGAGACCTGGAAACG
GCGGAGATCGCCATCAAGGCGGCCCAGACCGGTCACATGGTGATGTCCACCCTGCACACCAACAGCGCGGCAGAAACTCT
GACGCGCCTGCGGAACATGGGGGTGCCCGCCTTCAACCTGGCGACCTCGGTCAATCTGATCATCGCTCAGCGACTGGCGC
GTAAGCTGTGCAACAAGTGCAAGAGAGTCCATGAGGTGCCGCGTGAGGCGTTATTGGCCGAAGGCTTTCGCGAGGAGGAG
GTCGGCAGTTTCACCCTTTACCAGCCCATCGGCTGCGAGGACTGCAATGCGGGTTATCGCGGGCGAGTGGGGATCTATGA
GGTGGTGAAGGTCACGCCAGCGTTGCAGCAAATGATCATGGAGGAGGGCAACTCCATCGAGATCGCGGCGCGGATGCGCA
AAGAGGGCTTCAATGATCTGCGGCGGTCAGGACTGGTGAAGGCGATGCAGGGGATTACCAGTCTGGAGGAGATCAATCGG
GTGACTAAGGACTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (188-455)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	59.715	99.468	0.594
pilB	Acinetobacter baylyi ADP1	59.537	99.468	0.592
pilB	Legionella pneumophila strain ERS1305867	54.246	100	0.555
pilB	Vibrio cholerae strain A1552	49.912	100	0.5
pilB	Vibrio campbellii strain DS40M4	49.734	99.823	0.496
pilB	Vibrio parahaemolyticus RIMD 2210633	49.376	99.468	0.491
pilF	Neisseria gonorrhoeae MS11	48.845	99.823	0.488
pilF	Thermus thermophilus HB27	36.964	99.291	0.367
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.048	93.085	0.363