Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   Maut_RS09070 Genome accession   NZ_CP017019
Coordinates   1772002..1773663 (-) Length   553 a.a.
NCBI ID   WP_025775118.1    Uniprot ID   A0AAC9HIF0
Organism   Moorella thermoacetica strain DSM 103132     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1767002..1778663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Maut_RS09050 (Maut_01847) - 1767935..1768564 (-) 630 WP_069589957.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Maut_RS09055 (Maut_01848) - 1768824..1769348 (-) 525 WP_069589959.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Maut_RS09060 (Maut_01849) - 1769565..1770773 (-) 1209 WP_069591500.1 type II secretion system F family protein -
  Maut_RS09065 (Maut_01850) pilT 1770969..1771988 (-) 1020 WP_069589960.1 type IV pilus twitching motility protein PilT Machinery gene
  Maut_RS09070 (Maut_01851) pilF 1772002..1773663 (-) 1662 WP_025775118.1 GspE/PulE family protein Machinery gene
  Maut_RS09075 (Maut_01852) aroB 1773669..1774763 (-) 1095 WP_069589961.1 3-dehydroquinate synthase -
  Maut_RS09080 (Maut_01853) - 1774742..1775263 (-) 522 WP_069589963.1 shikimate kinase -
  Maut_RS09085 (Maut_01854) aroC 1775316..1776467 (-) 1152 WP_069589965.1 chorismate synthase -
  Maut_RS09090 (Maut_01855) - 1776492..1777379 (-) 888 WP_069589967.1 shikimate dehydrogenase -
  Maut_RS09095 (Maut_01856) - 1777499..1778020 (-) 522 WP_025775125.1 YqeG family HAD IIIA-type phosphatase -

Sequence

Protein


Download         Length: 553 a.a.        Molecular weight: 60486.17 Da        Isoelectric Point: 6.2155

>NTDB_id=194539 Maut_RS09070 WP_025775118.1 1772002..1773663(-) (pilF) [Moorella thermoacetica strain DSM 103132]
MDSRRRLGDLLIEAGMLTPAQLEQALQEQKRSGERLGKVLIRLGFITEASMLEVLEFQLGIPKVVLADYHLDPEVVRLVP
EGLARRYQAIPIRLDGNRLLVAMADPLNLVALDDLRLVTGKEIMPAIAAEKEIEAALSRFWQREPVTSMSEVAAAVAAAE
SGGRAGGTEGAPAVRLVNSFIQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEK
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKIVLRLLDQEAMLLPLDDLGFLPAIKERFESLIHSSYGMLLITGPTGSGK
TTTLYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPKAGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATT
GHLVLTTLHTNDAAGAVTRLLDMGVEGYLVNSSLIGVVAQRLVRRICPHCREMYEPEPGSPERAWLPGEERLWRGRGCEN
CHYTGYANRTAIQEVLVMNEELRRLVAAKAPATALKEAAVAGGMVPLIDDGLEKARQGITTVSEVLRVSLGGL

Nucleotide


Download         Length: 1662 bp        

>NTDB_id=194539 Maut_RS09070 WP_025775118.1 1772002..1773663(-) (pilF) [Moorella thermoacetica strain DSM 103132]
ATGGATAGTCGACGACGACTGGGGGACCTGTTGATCGAAGCCGGGATGCTTACCCCGGCCCAGCTGGAACAGGCCCTGCA
GGAACAGAAACGCAGCGGGGAGCGCCTGGGTAAGGTTTTAATCCGCCTGGGATTTATCACCGAGGCCAGCATGCTGGAGG
TCCTGGAGTTCCAGCTGGGGATCCCCAAGGTGGTCCTGGCTGACTACCACCTGGATCCGGAGGTGGTCCGCCTGGTGCCG
GAAGGCCTGGCCCGGCGCTACCAGGCCATCCCCATCCGCCTGGACGGCAACCGCCTCCTGGTGGCCATGGCCGATCCCCT
GAACCTCGTGGCCCTGGACGACCTGCGCCTGGTCACCGGCAAGGAGATTATGCCGGCTATAGCCGCCGAGAAGGAAATCG
AGGCAGCTTTAAGCCGGTTCTGGCAACGGGAACCCGTTACGAGCATGAGCGAAGTAGCGGCAGCCGTCGCCGCCGCGGAA
TCTGGCGGGCGCGCCGGGGGCACGGAAGGCGCGCCGGCTGTGCGCCTGGTCAACAGTTTTATCCAGCAGGCCATCCAGAC
CCGGGCCAGCGACATCCATATAGAGCCCCAGGAGGGGGAGGTCCGGGTGCGCCTGCGGGTAGACGGCCTGCTGCGGGAGT
TGACCCGCCTGCCCCTGGGGGTTTTAAGTAGCCTGATCTCCAGGATCAAGATCATGGCCGGCATGGACATCGCCGAAAAA
CGCTTGCCCCAGGACGGCCGTTTTCAGTTTACCCTGGGTAAACGCAGTGTCGACCTCAGGGTTTCCAGCCTGCCTACTGT
TTACGGCGAAAAGATCGTCCTGCGCCTCCTGGACCAGGAGGCCATGCTCCTGCCCCTGGACGACCTGGGATTTTTGCCGG
CCATAAAAGAACGCTTTGAGAGCCTCATCCACAGTTCCTACGGTATGCTCCTCATTACCGGTCCCACGGGCAGCGGTAAG
ACGACGACCCTCTATGCCACCCTTAACATTTTAAGCTCGCCGGAAAAAAATATCATTACCATTGAGGATCCGGTAGAATA
CCTGCTGCCCGGCATCAATCAGGTGCGGGTTAACCCCAAGGCCGGCCTGACCTTTGCTTCAGGGCTGCGTTCCATCCTGC
GTCAGGACCCGGATATCATTATGGTCGGGGAGATTCGCGACCGGGAGACGGCCGATATCGCCGTCCGGGCGGCGACTACC
GGTCACCTGGTCTTAACGACCCTGCACACCAATGACGCCGCCGGCGCCGTAACCCGCCTCCTGGATATGGGAGTGGAAGG
CTACCTGGTCAATTCCTCCCTTATTGGCGTGGTGGCCCAGCGCCTGGTGCGCCGCATCTGTCCCCATTGCCGGGAGATGT
ACGAGCCGGAGCCGGGCTCTCCGGAAAGGGCCTGGTTGCCGGGCGAGGAACGGCTCTGGCGCGGCCGGGGTTGCGAAAAC
TGCCATTATACCGGTTACGCCAACCGGACGGCCATCCAGGAGGTCCTGGTCATGAATGAAGAACTCCGGCGCCTGGTAGC
CGCCAAGGCGCCGGCTACGGCCCTGAAGGAGGCAGCGGTGGCCGGCGGTATGGTTCCTTTGATTGACGACGGTTTGGAAA
AAGCCCGCCAGGGGATCACTACGGTGAGCGAGGTCCTACGCGTTTCCCTGGGAGGTTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)

Type II/IV secretion system protein (T2SSE)

(180-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

49.18

99.277

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.273

99.458

0.45
  pilB Vibrio campbellii strain DS40M4

41.831

100

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

41.281

100

0.42
  pilB Vibrio cholerae strain A1552

41.877

100

0.42
  pilB/pilB1 Synechocystis sp. PCC 6803

37.931

100

0.418
  pilB Acinetobacter baylyi ADP1

40.541

100

0.407
  pilB Acinetobacter baumannii D1279779

39.421

100

0.394
  pilF Neisseria gonorrhoeae MS11

38.321

99.096

0.38
  pilB Legionella pneumophila strain ERS1305867

41.616

89.512

0.373

Multiple sequence alignment