Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   A9P79_RS25630 Genome accession   NZ_CP016950
Coordinates   2948249..2949973 (+) Length   574 a.a.
NCBI ID   WP_240072666.1    Uniprot ID   -
Organism   Cupriavidus taiwanensis strain SWF66294     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2943249..2954973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9P79_RS25590 (A9P79_25225) obgE 2943829..2944926 (-) 1098 WP_062801793.1 GTPase ObgE -
  A9P79_RS25595 (A9P79_25230) rpmA 2945047..2945307 (-) 261 WP_115704864.1 50S ribosomal protein L27 -
  A9P79_RS25600 (A9P79_25235) rplU 2945347..2945658 (-) 312 WP_240072663.1 50S ribosomal protein L21 -
  A9P79_RS25605 - 2945855..2946088 (-) 234 WP_147299810.1 hypothetical protein -
  A9P79_RS25610 (A9P79_25240) ispB 2946138..2947067 (+) 930 WP_240074671.1 octaprenyl diphosphate synthase -
  A9P79_RS25620 (A9P79_25250) - 2947321..2947641 (-) 321 WP_240072664.1 helix-turn-helix domain-containing protein -
  A9P79_RS25625 (A9P79_25255) - 2947654..2947929 (-) 276 WP_240072665.1 type II toxin-antitoxin system RelE/ParE family toxin -
  A9P79_RS25630 (A9P79_25260) pilF 2948249..2949973 (+) 1725 WP_240072666.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A9P79_RS25635 (A9P79_25265) - 2950039..2951310 (+) 1272 WP_240072667.1 type II secretion system F family protein -
  A9P79_RS25640 (A9P79_25270) pilD 2951314..2952225 (+) 912 WP_115704873.1 prepilin peptidase Machinery gene
  A9P79_RS25645 (A9P79_25275) coaE 2952310..2952936 (+) 627 WP_115704875.1 dephospho-CoA kinase -
  A9P79_RS25650 (A9P79_25280) zapD 2953146..2953904 (+) 759 WP_115681185.1 cell division protein ZapD -
  A9P79_RS25655 (A9P79_25285) - 2953922..2954110 (+) 189 WP_111519102.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63279.94 Da        Isoelectric Point: 7.2932

>NTDB_id=193961 A9P79_RS25630 WP_240072666.1 2948249..2949973(+) (pilF) [Cupriavidus taiwanensis strain SWF66294]
MTLGLALAQSRRIAPALLAQLEQAARDKQSQLIDEIIGSGTMSAHDVALFAADKYQLPLLDLAQYNLTKVPPALAGNREF
HAHRLLPLGRRENRLVLALSDPSNQAGLDAIRDKYKLPVEAVVVEHDKLMKHVRAAGEALGTLKNISPVQAERKMIEYDP
VAAAANPRNRTTADNIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKGKDAKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCSCKREGEIPREALL
EAGFREHDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQEIILAHGTALQIAEQARRDGVLSLREAGLLKVRQ
GVTSLEEVLATTNT

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=193961 A9P79_RS25630 WP_240072666.1 2948249..2949973(+) (pilF) [Cupriavidus taiwanensis strain SWF66294]
ATGACACTCGGACTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCGCGTGA
TAAGCAATCGCAGCTCATCGACGAGATCATCGGCAGCGGCACCATGAGCGCGCATGACGTGGCGCTGTTCGCCGCGGACA
AGTACCAGCTGCCTCTGCTCGATCTGGCCCAGTACAACCTGACCAAGGTGCCGCCGGCGCTGGCCGGCAACCGTGAGTTC
CACGCCCACCGGCTGCTGCCGCTGGGCCGGCGCGAGAACCGCCTGGTGCTGGCGCTGTCCGATCCGTCCAACCAGGCCGG
CCTGGACGCGATCCGCGACAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCATGTGC
GCGCCGCCGGCGAGGCGCTGGGCACGCTGAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGATCCG
GTCGCCGCCGCCGCCAACCCGCGCAACCGCACCACGGCCGACAATATCGACGACGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCACCGCGGCGCCTCGGACCTGCACTTCGAACCGTTCGAAACCTTTTACCGCATCCGCTTCC
GCGTCGATGGCGTGCTGCAGGAGGTCGCGCGCCCGCCGCTGGACATCCGCGACAAGATCGCGACCCGCATCAAGGTGCTG
TCGCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGGCAAGGA
TGCCAAGGAAACCGTCGAGAAGGCGGTGGACTTCCGCGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATCCTGGAATCGTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGGCGCTGCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCTACCGGCAGCGGCAAGACCGTGTCGCTGTACACCTTCCT
GAACCTGCTGAACCAGGGCGACATCAATATCTCGACGGCCGAGGACCCGGCTGAAATCCAGCTGCCGGGCATCAACCAGG
TCAACGTCAACGACAAGGCCGGGCTGACCTTTGCCGCCGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACCTGGTGTTGTCGACGCT
GCACACCAACGACGCGCCGACCACGCTGACGCGCCTGATGAACATGGGCGTGGCCCCCTTCAATATCGCGTCGAGCGTGC
TGATGATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGCAGCTGCAAGCGCGAGGGCGAGATCCCGCGCGAGGCCTTGCTG
GAAGCGGGCTTCCGCGAGCACGACCTGGACGGCAGCTGGCAGCCCTACCATCCGGTCGGCTGCGAGCGCTGCAACGGCAG
CGGCTACAAGGGCCGCTGCGGCATCTACCAGGTGATGCCGATCACCGAGGCCATGCAGGAAATCATCCTGGCGCACGGCA
CCGCGCTTCAGATTGCCGAGCAGGCGCGCAGGGACGGCGTGCTATCGTTGCGCGAAGCGGGGCTGCTGAAGGTCAGGCAG
GGCGTCACGTCACTCGAAGAAGTGCTGGCGACCACGAACACGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(187-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

51.986

100

0.524
  pilB Acinetobacter baumannii D1279779

51.916

100

0.519
  pilB Acinetobacter baylyi ADP1

52.698

96.864

0.51
  pilB Legionella pneumophila strain ERS1305867

49.009

96.69

0.474
  pilB Vibrio cholerae strain A1552

45.043

100

0.451
  pilB Vibrio campbellii strain DS40M4

45.656

94.251

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

45.287

94.251

0.427
  pilF Thermus thermophilus HB27

38.687

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.919

96.69

0.376

Multiple sequence alignment