Detailed information NTG0193346

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: XJ27_RS21980
• Genome accession: NZ_CP016878
• Coordinates: 4898042..4899778 (+)
• Length: 578 a.a.
• NCBI ID: WP_074058080.1
• Uniprot ID: -
• Organism: Xanthomonas hortorum strain B07-007
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	4896880..4897842	4898042..4899778	flank	200

Gene organization within MGE regions

Location: 4896880..4899778

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XJ27_RS21975 (XJ27_20990)	-	4896880..4897842 (-)	963	WP_006448937.1	IS1595 family transposase	-
XJ27_RS21980 (XJ27_20995)	pilB	4898042..4899778 (+)	1737	WP_074058080.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62751.97 Da
• Isoelectric Point: 5.4464

>NTDB_id=193346 XJ27_RS21980 WP_074058080.1 4898042..4899778(+) (pilB) [Xanthomonas hortorum strain B07-007]
MSLTGTANLVGITGIARRLVQDGVLDEALARVAMDQAASAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
DDDDDMGDLDVSSGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLP
EHALLAEGFTAEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=193346 XJ27_RS21980 WP_074058080.1 4898042..4899778(+) (pilB) [Xanthomonas hortorum strain B07-007]
ATGAGTCTTACGGGAACAGCTAATTTGGTTGGAATAACTGGGATTGCTAGACGTCTCGTTCAGGACGGAGTGCTTGACGA
AGCATTAGCCCGTGTTGCTATGGATCAGGCGGCATCTGCGAAAGTTCCGTTGCCTCAGTGGTTTGCGGAAAAAAAGCTAG
TCACTGCCTCCCAACTCGCCGCCGCCAATGCGGTTGAGTTCGGCATGCCACTGATGGACGTGTCGGTATTCGACGCCAGC
CAGAACGCAGTCAAGCTGGTCAGCGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCAACCCAGACCCGAGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTGGAGCCGA
TTCTGGTCGATGAGGACCAGATCCGCCGCACATTGGAGCAGTGGCAGGCAAGCAATGCATCGCTAGGCTCGTCGCTCGGT
GACGATGATGACGATATGGGGGATCTGGACGTCTCCTCCGGGGACGAGGACATGGGCGCCGGTGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCTTCCGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGGATCGACGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGTTGAGCCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGTATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAGAAGCTGGGCTATGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCATAAGCCTTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCTTGAACGACGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GTGTGAATCAGGTGCAGCAGAACGTGAAGCGCGGCATGACGTTTGCTGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCAATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATTGCACCGTACAACATCA
CTTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGGCGCCGCTGCCC
GAGCACGCGCTGTTGGCCGAGGGGTTCACCGCTGAGCAGATCGCTGCCGGAATCGAACTGTACGAGGCGGTTGGTTGCGA
CGAATGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATTGGCGCGATCGTGC
TGGAAGGCGGTAATGCAATGCAGATCGCAGAGGCCGCGCAGAGGATCGGCATCCGCGATTTGCGGCAGTCGGCGCTGGTG
AAGGCGGCGCATGGAGTGACGAGCCTGGCTGAGATCAATCGGGTGACAAAGGATTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-153)

• Type II/IV secretion system protein (T2SSE) (201-468)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.986	98.27	0.55
pilB	Acinetobacter baylyi ADP1	54.577	98.27	0.536
pilB	Legionella pneumophila strain ERS1305867	52.197	98.443	0.514
pilB	Vibrio cholerae strain A1552	48.517	99.135	0.481
pilF	Neisseria gonorrhoeae MS11	48.239	98.27	0.474
pilB	Vibrio campbellii strain DS40M4	45.518	98.443	0.448
pilB	Vibrio parahaemolyticus RIMD 2210633	45.567	97.578	0.445
pilB/pilB1	Synechocystis sp. PCC 6803	35.599	100	0.381
pilF	Thermus thermophilus HB27	40.691	90.138	0.367