Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BSY15_RS03395 Genome accession   NZ_CP016447
Coordinates   771021..772760 (+) Length   579 a.a.
NCBI ID   WP_069103615.1    Uniprot ID   -
Organism   Acidovorax sp. RAC01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766021..777760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSY15_RS03365 (BSY15_685) proB 766535..767677 (-) 1143 WP_069103611.1 glutamate 5-kinase -
  BSY15_RS03370 (BSY15_686) obgE 767768..768838 (-) 1071 WP_069103612.1 GTPase ObgE -
  BSY15_RS03375 (BSY15_687) rpmA 769033..769290 (-) 258 WP_069103613.1 50S ribosomal protein L27 -
  BSY15_RS03380 (BSY15_688) rplU 769307..769618 (-) 312 WP_005794184.1 50S ribosomal protein L21 -
  BSY15_RS03385 (BSY15_689) - 769792..770775 (+) 984 WP_231940697.1 polyprenyl synthetase family protein -
  BSY15_RS03395 (BSY15_691) pilB 771021..772760 (+) 1740 WP_069103615.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BSY15_RS03400 (BSY15_692) pilC 772780..773994 (+) 1215 WP_069103616.1 type II secretion system F family protein Machinery gene
  BSY15_RS03405 (BSY15_693) - 773994..774866 (+) 873 WP_069103617.1 A24 family peptidase -
  BSY15_RS03410 (BSY15_694) coaE 774902..775510 (+) 609 WP_069103618.1 dephospho-CoA kinase -
  BSY15_RS03415 (BSY15_695) zapD 775601..776356 (+) 756 WP_069106358.1 cell division protein ZapD -
  BSY15_RS20515 - 776422..776640 (+) 219 WP_083235534.1 DNA gyrase inhibitor YacG -
  BSY15_RS03420 (BSY15_696) - 776891..777319 (-) 429 WP_156779046.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62882.15 Da        Isoelectric Point: 5.1557

>NTDB_id=188312 BSY15_RS03395 WP_069103615.1 771021..772760(+) (pilB) [Acidovorax sp. RAC01]
MAAVDTAPKEASAVALPGLGRALISAGKLTQKSAEDIYKKSQIARTSFIGELTGSGAVSAADLAHTVSAVFGAPLLDLEA
IDPLRLPRELLDPKICQAYRVIVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLSKLVDATTKSVSDSM
DGMTASGGDFEFDDVPVEENPESNDPGAADVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASP
PVAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPVEK
ERLLQAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPADI
PHETLVEAGYPEEELDGTWVTYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQARAEGVRSLRDSGL
YKAKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=188312 BSY15_RS03395 WP_069103615.1 771021..772760(+) (pilB) [Acidovorax sp. RAC01]
ATGGCCGCTGTCGATACTGCCCCCAAAGAAGCCTCCGCGGTCGCGCTTCCCGGTTTGGGGAGGGCACTCATATCCGCGGG
CAAGCTGACTCAAAAGTCCGCTGAGGATATTTACAAAAAATCACAGATTGCCCGTACCAGCTTTATCGGCGAACTGACGG
GCTCTGGAGCGGTGTCAGCCGCCGACCTGGCACACACCGTCTCAGCGGTGTTTGGCGCCCCGCTTCTGGACCTAGAAGCC
ATCGACCCATTGCGTTTACCCAGGGAGCTACTGGATCCGAAAATCTGCCAGGCCTATCGCGTGATAGTGCTTAGCAAGCG
AAATAACAGACTGATTGTCGCCACTGCCGACCCAACGGATCAGGAAGCGGCGGAAAAAATCAAGTTCACGACTCAGATGG
GGGTGGACTGGATTATCGCCGAGTATGACAAGCTGAGTAAGCTGGTTGATGCCACGACGAAATCGGTGTCTGACTCCATG
GACGGCATGACAGCGTCGGGTGGCGATTTTGAATTCGATGACGTACCGGTAGAAGAAAATCCTGAGTCAAATGATCCTGG
CGCAGCAGATGTTGAAGATGCGCCGGTCGTAAAGTTCTTGCACAAGATGCTTCTTGATGCATTTAACATGCGTGCGTCAG
ACCTGCACTTCGAACCTTACGAGCATCAATACCGAGTACGTTTCCGAATCGACGGGGAGCTCCGCGAAATCGCTTCACCT
CCCGTTGCCATCAAGGACAAACTCGCTTCACGGATCAAGGTGATATCCCGTCTTGATATCTCCGAAAAACGCGTTCCTCA
AGACGGCCGGATGAAACTCAAGGTCGGCCCTGACCGAGTTATCGATTTTCGCGTAAGCACCTTGCCCACTCTCTTTGGCG
AGAAAATTGTGATTCGTATCCTCGATCCCAGCAGTGCCAAGCTGGGTATCGATGCGCTGGGCTATGAACCCGTAGAAAAA
GAGCGGCTTCTGCAGGCTATCGGCAGACCGTACGGGATGATTCTCGTGACCGGACCGACAGGTTCAGGAAAAACTGTTTC
ACTCTACACCTGTCTCAATTTATTGAATAAACCTGGCGTCAACATTGCCACAGCAGAAGATCCCTCTGAAATCAACCTGC
CAGGTGTGAATCAGGTCAACGTCAATGAAAAGGCTGGACTGACTTTCGCCGTCGCTCTGAAGTCTTTCTTGCGTCAAGAT
CCTGACATTATCATGGTCGGCGAAATTCGCGACCTGGAGACCGCCGACATTTCGATCAAGGCCGCACAAACTGGACATTT
GGTCCTGTCAACGCTGCATACCAACGACGCCCCCACCACCTTGACGCGTATGCGCAACATGGGGATCGCCCCATTCAATA
TCGCCTCAAGCGTGATTCTGATCACTGCGCAGAGATTGGCTCGACGACTATGCCCCAACTGCAAGGCACCGGCAGACATT
CCGCACGAGACATTGGTAGAGGCGGGCTATCCCGAAGAAGAGTTGGACGGGACATGGGTCACCTACAAACCCGTCGGCTG
CTCTGCGTGCAATAACGGTTACAAAGGGCGAGTGGGCATCTACCAGGTGATGCCCATATCCGAGGAGATTCAACGAATCA
TCCTTCGCGATGGAAGTGCCCTTGAAATTGCGGAACAGGCGCGGGCCGAAGGCGTCAGATCATTACGAGATTCGGGTTTG
TACAAGGCGAAATTGGGATTAACTTCTCTGGAAGAAGTGCTCGCAGTCACTAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.516

100

0.539
  pilB Acinetobacter baumannii D1279779

52.65

100

0.532
  pilF Neisseria gonorrhoeae MS11

53.097

97.582

0.518
  pilB Legionella pneumophila strain ERS1305867

49.645

97.409

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.15

97.927

0.442
  pilB Vibrio campbellii strain DS40M4

45.374

97.064

0.44
  pilB Vibrio cholerae strain A1552

47.448

91.364

0.434
  pilF Thermus thermophilus HB27

39.076

97.237

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.22

98.964

0.378

Multiple sequence alignment