Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   clem_RS06495 Genome accession   NZ_CP016397
Coordinates   1491231..1492931 (-) Length   566 a.a.
NCBI ID   WP_094090888.1    Uniprot ID   A0A222P288
Organism   Legionella clemsonensis strain CDC-D5610     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1486231..1497931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  clem_RS06475 (clem_06580) - 1486772..1487914 (+) 1143 WP_094090885.1 Fic family protein -
  clem_RS06480 (clem_06585) - 1488174..1489193 (-) 1020 WP_198333205.1 carbonic anhydrase -
  clem_RS06485 (clem_06590) - 1489319..1490176 (-) 858 WP_094090886.1 A24 family peptidase -
  clem_RS06490 (clem_06595) pilC 1490182..1491231 (-) 1050 WP_094090887.1 type II secretion system F family protein Machinery gene
  clem_RS06495 (clem_06600) pilB 1491231..1492931 (-) 1701 WP_094090888.1 type IV-A pilus assembly ATPase PilB Machinery gene
  clem_RS06500 (clem_06605) - 1493034..1493264 (+) 231 WP_094090889.1 hypothetical protein -
  clem_RS06505 (clem_06610) - 1493413..1494150 (+) 738 WP_094090890.1 class I SAM-dependent methyltransferase -
  clem_RS06510 (clem_06615) - 1494320..1494646 (+) 327 WP_094090891.1 DUF1820 family protein -
  clem_RS06515 (clem_06620) - 1494670..1495230 (+) 561 WP_094090892.1 hypoxanthine-guanine phosphoribosyltransferase -
  clem_RS06520 (clem_06625) - 1495399..1496115 (-) 717 WP_232505579.1 murein L,D-transpeptidase family protein -
  clem_RS06525 (clem_06630) - 1496222..1496812 (-) 591 WP_094090893.1 type I secretion system protein LssZ -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 63956.64 Da        Isoelectric Point: 7.4073

>NTDB_id=187969 clem_RS06495 WP_094090888.1 1491231..1492931(-) (pilB) [Legionella clemsonensis strain CDC-D5610]
MERIQQEQQHLSLVAQLINKNNLISEKEIAQYQQNADQADQSLQNYLVTNNIVSELQIALLLAEHFNIPYLNIDSVDIEL
IPWRLITDRLMRRLHVAPLMLRENHLILATDYPTQTLTKEIQFYTGLTVTLAIAETSKLNQLIEKIYQRKLSEGPTNYKD
YSMQQASSNLSVEVKDCSEDDASIVNFVDSILFDAIKRKASDIHFETYENYYRIRYRMDGVLTEIATPPIDLAQRITARI
KIMANLDISERRIPQDGRFNVRFSENTLRDCRINSCPTINGEKVVIRILNSDTPKINIESLGFNKRQEKYFLDAIAKPQG
MILVTGPTGSGKTVSLYAALSLLNTQEKNISTAEDPVEIKLSGINQVNINTKAGLTFAKALRAFLRQDPDVMMIGEIRDF
ETAEMAIKAAQTGHLILSTLHTNSAAETLTRLANLGVSPFNIASSLTLLIAQRLVRRLCQHCKQIQSNLTTHQLEQFGLN
AKISSIPFFKAKGCKYCFDGYKGRVGIFEVMPVSPAIERLILTGKDTDEILKQAKKEGMLTIYQSGLEKIKEGITTLEEL
NRVVIQ

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=187969 clem_RS06495 WP_094090888.1 1491231..1492931(-) (pilB) [Legionella clemsonensis strain CDC-D5610]
ATGGAAAGAATTCAGCAAGAACAACAACATTTATCTCTTGTTGCTCAACTTATTAATAAGAATAATCTAATCAGTGAAAA
AGAAATAGCTCAGTATCAGCAAAATGCTGATCAAGCGGATCAATCTCTTCAGAATTATTTAGTTACAAATAATATTGTTT
CTGAATTACAAATAGCATTACTGCTAGCAGAACATTTCAACATTCCCTATCTCAATATAGACTCTGTAGATATTGAACTC
ATTCCCTGGCGTCTTATCACCGACAGGCTAATGCGTCGGCTTCACGTAGCACCACTTATGCTACGAGAAAATCATTTAAT
TCTAGCAACCGATTATCCTACTCAAACACTAACGAAAGAAATTCAATTCTATACTGGGCTAACAGTAACTTTGGCTATTG
CTGAGACTTCCAAGCTAAATCAACTTATTGAAAAAATTTATCAACGCAAACTCAGCGAGGGCCCAACTAATTACAAAGAT
TATTCCATGCAGCAAGCTTCAAGTAATTTATCGGTTGAAGTGAAGGACTGTTCTGAAGATGACGCATCCATTGTTAATTT
TGTCGACAGTATTCTTTTTGACGCTATTAAAAGAAAGGCCTCTGACATTCATTTCGAGACCTATGAAAATTACTATCGAA
TTCGTTATCGTATGGATGGTGTTTTAACCGAGATAGCAACACCTCCAATTGATTTAGCTCAAAGGATTACGGCACGTATA
AAAATTATGGCTAATTTAGATATCTCTGAGCGCCGTATCCCTCAGGATGGTCGATTTAATGTAAGATTTTCAGAAAATAC
TTTAAGGGATTGTCGGATAAATAGTTGTCCCACTATTAACGGTGAAAAAGTAGTGATTCGCATTCTTAATTCAGATACAC
CAAAAATTAACATTGAATCCTTGGGATTTAACAAAAGACAGGAAAAATACTTTCTGGATGCCATTGCAAAGCCGCAAGGA
ATGATTCTTGTAACGGGCCCAACTGGCAGTGGTAAAACAGTTTCCTTATATGCAGCCCTTAGTTTATTAAATACCCAGGA
AAAAAATATTTCCACTGCAGAAGATCCTGTAGAGATTAAACTCTCAGGAATTAATCAAGTAAATATTAATACAAAAGCAG
GCTTAACCTTTGCAAAAGCTTTGCGTGCTTTTTTACGTCAAGATCCCGATGTGATGATGATAGGCGAAATCCGAGATTTT
GAAACAGCTGAGATGGCGATTAAAGCAGCTCAAACAGGTCATCTAATTTTGTCTACGTTACATACTAATAGTGCTGCCGA
AACTTTAACACGGCTTGCTAACTTAGGAGTTTCTCCCTTTAATATTGCAAGCTCTCTTACTCTTCTAATAGCTCAGCGCC
TGGTAAGACGTTTATGCCAGCACTGCAAGCAAATTCAAAGCAATTTGACAACACATCAATTAGAACAATTTGGTTTGAAC
GCAAAAATATCATCAATTCCTTTTTTCAAAGCTAAAGGATGCAAATATTGCTTTGATGGCTATAAGGGACGTGTTGGGAT
ATTTGAGGTGATGCCTGTCTCGCCTGCCATTGAGAGACTTATTCTTACAGGCAAGGATACTGATGAAATTCTTAAACAGG
CGAAAAAAGAAGGCATGCTCACTATTTATCAATCAGGACTTGAAAAAATTAAAGAAGGAATAACAACCCTTGAGGAATTG
AATCGAGTGGTAATTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222P288

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.049

100

0.542
  pilB Acinetobacter baylyi ADP1

46.126

98.057

0.452
  pilB Acinetobacter baumannii D1279779

45.213

99.647

0.451
  pilB Vibrio cholerae strain A1552

48.532

90.283

0.438
  pilB Vibrio parahaemolyticus RIMD 2210633

45.301

93.993

0.426
  pilF Neisseria gonorrhoeae MS11

44.037

96.29

0.424
  pilB Vibrio campbellii strain DS40M4

48.34

85.159

0.412
  pilF Thermus thermophilus HB27

38.571

98.94

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.926

100

0.369

Multiple sequence alignment