Detailed information NTG0187922

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BBJ33_RS12555
• Genome accession: NZ_CP016395
• Coordinates: 2623236..2623850 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain M75
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2618236..2628850

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BBJ33_RS12530 (BBJ33_12530)	rpsT	2618536..2618802 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BBJ33_RS12535 (BBJ33_12535)	holA	2618819..2619860 (-)	1042	Protein_2488	DNA polymerase III subunit delta	-
BBJ33_RS20280	-	2619900..2620054 (-)	155	Protein_2489	hypothetical protein	-
BBJ33_RS12540 (BBJ33_12540)	-	2620095..2620229 (+)	135	WP_003152870.1	YqzM family protein	-
BBJ33_RS12545 (BBJ33_12545)	comEC	2620248..2622599 (-)	2352	WP_069473513.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BBJ33_RS12550 (BBJ33_12550)	-	2622600..2623169 (-)	570	WP_003152868.1	ComE operon protein 2	-
BBJ33_RS12555 (BBJ33_12555)	comEA	2623236..2623850 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
BBJ33_RS12560 (BBJ33_12560)	comER	2623909..2624730 (+)	822	WP_014305449.1	late competence protein ComER	-
BBJ33_RS12565 (BBJ33_12565)	-	2624799..2625536 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
BBJ33_RS12570 (BBJ33_12570)	rsfS	2625533..2625889 (-)	357	WP_003152864.1	ribosome silencing factor	-
BBJ33_RS12575 (BBJ33_12575)	yqeK	2625886..2626467 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BBJ33_RS12580 (BBJ33_12580)	-	2626457..2627026 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
BBJ33_RS12585 (BBJ33_12585)	yhbY	2627037..2627327 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BBJ33_RS12590 (BBJ33_12590)	aroE	2627321..2628163 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=187922 BBJ33_RS12555 WP_003152867.1 2623236..2623850(-) (comEA) [Bacillus velezensis strain M75]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=187922 BBJ33_RS12555 WP_003152867.1 2623236..2623850(-) (comEA) [Bacillus velezensis strain M75]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363