Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VSVS12_RS13620 Genome accession   NZ_CP016307
Coordinates   3026232..3027914 (+) Length   560 a.a.
NCBI ID   WP_065430954.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain VS-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3021232..3032914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VSVS12_RS13605 (VSVS12_02760) pncC 3022371..3022859 (-) 489 WP_005593984.1 nicotinamide-nucleotide amidase -
  VSVS12_RS13610 (VSVS12_02761) mutS 3022944..3025511 (+) 2568 WP_065430952.1 DNA mismatch repair protein MutS -
  VSVS12_RS13615 (VSVS12_02762) pilA 3025774..3026232 (+) 459 WP_065430953.1 pilin Machinery gene
  VSVS12_RS13620 (VSVS12_02763) pilB 3026232..3027914 (+) 1683 WP_065430954.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VSVS12_RS13625 (VSVS12_02764) pilC 3027968..3029191 (+) 1224 WP_065430955.1 type II secretion system F family protein Machinery gene
  VSVS12_RS13630 (VSVS12_02765) - 3029297..3030165 (+) 869 Protein_2609 prepilin peptidase -
  VSVS12_RS13635 (VSVS12_02766) coaE 3030170..3030778 (+) 609 WP_065430956.1 dephospho-CoA kinase -
  VSVS12_RS13640 (VSVS12_02767) zapD 3030813..3031553 (+) 741 WP_005593976.1 cell division protein ZapD -
  VSVS12_RS13645 (VSVS12_02768) yacG 3031629..3031823 (+) 195 WP_005593974.1 DNA gyrase inhibitor YacG -
  VSVS12_RS13650 (VSVS12_02769) rplS 3032182..3032535 (-) 354 WP_005593972.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61834.95 Da        Isoelectric Point: 6.8553

>NTDB_id=186772 VSVS12_RS13620 WP_065430954.1 3026232..3027914(+) (pilB) [Vibrio scophthalmi strain VS-12]
MSHNLATLLSQQGLLAHTQVGQPPFTSNHPSGFDPSILEFISADKLAAVVSQLFKLPQVNIAHYEYRTVCEELGLSTLIQ
KHLSLPLAKTNGTLTLAVVDPTLDVIEDEFRFATGLQIELVVTDYNAIKKAIYALYGRSLQSTTTAKKEINALELAQLVN
VSEEEFGREEDLSLDDAPVSRYIHQILLEAIRKGASDIHFEPYEKHYRIRLRCDGLLVESHQPASQLGRRLSTRLKILAN
LDIAERRRPQDGRSKVKLNADSAVDMRISTLPTLWGEKVVLRLLESGINLLDIDRLGYSHQQKQHYLKALKQPQGLILFT
GPTGSGKTISLYSGLNLLNTTQVNIATAEDPVEINLSGINQVQIQPQIGFDFACALRSFLRQDPDIVMVGEIRDVETADI
AIKASQTGHLVLSTLHTNSAADTIVRLASMGIKTYDLAASLTLVMAQRLVRKLCAYCKEPAKRNIDAKDDLLGPVTEQQY
QANQHGCTQCNQGYQGRIGLFEVLPINAKLQQAILQCATAIEIETLAKNEGMMSLQQSGIAALNAGKTSRQELQRVLLFG

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=186772 VSVS12_RS13620 WP_065430954.1 3026232..3027914(+) (pilB) [Vibrio scophthalmi strain VS-12]
ATGAGTCATAATTTAGCGACGCTGCTTTCTCAACAAGGCCTACTTGCTCACACGCAAGTAGGCCAGCCACCTTTTACCTC
AAACCATCCTTCTGGCTTTGATCCGTCGATACTAGAATTCATTAGCGCAGATAAATTAGCCGCTGTGGTAAGCCAATTAT
TCAAACTGCCACAGGTTAATATTGCTCACTATGAGTATCGTACGGTTTGTGAAGAACTGGGGTTGAGTACACTGATCCAA
AAACACCTTTCATTACCACTTGCCAAAACCAATGGCACGCTCACGCTGGCAGTGGTCGATCCGACACTGGATGTGATTGA
AGACGAGTTTCGTTTTGCGACTGGATTGCAGATTGAATTAGTGGTGACTGACTACAATGCTATCAAGAAGGCCATTTATG
CCCTCTATGGCCGAAGCCTTCAATCCACCACAACCGCGAAGAAAGAGATCAATGCCCTCGAACTGGCACAGCTAGTGAAT
GTCAGCGAAGAGGAGTTTGGTCGAGAAGAAGATTTGAGCCTAGATGACGCCCCCGTTAGTCGCTACATTCATCAGATATT
ACTCGAGGCGATACGCAAAGGGGCTTCTGATATTCATTTTGAACCGTATGAAAAGCATTACCGGATTCGCCTACGCTGCG
ACGGACTACTCGTAGAAAGCCACCAGCCAGCTTCTCAACTTGGCCGCCGCCTCTCCACACGGTTGAAAATATTAGCCAAC
TTAGATATAGCTGAACGTCGACGACCGCAAGATGGCAGAAGCAAGGTGAAGCTAAATGCTGATAGCGCCGTGGATATGCG
AATCTCGACGCTACCCACTTTATGGGGTGAAAAGGTGGTGCTGCGATTATTGGAAAGCGGTATCAACCTGCTTGATATTG
ACCGACTCGGCTATAGCCACCAGCAAAAACAGCACTACTTAAAAGCACTCAAACAACCACAAGGGCTGATATTGTTTACT
GGCCCAACAGGCAGCGGTAAAACCATCTCACTCTATAGTGGGCTAAATCTTCTTAACACGACGCAAGTCAATATTGCCAC
CGCAGAAGATCCAGTAGAGATCAATCTCAGCGGTATCAATCAAGTCCAAATTCAACCGCAAATCGGCTTCGACTTCGCTT
GCGCACTGCGATCTTTTCTTCGTCAAGATCCAGATATTGTTATGGTCGGCGAAATTCGTGATGTTGAAACCGCTGACATT
GCGATCAAAGCGTCACAAACGGGCCACTTGGTTCTTTCCACTTTGCACACCAACTCCGCAGCTGACACCATCGTTCGTTT
AGCGAGTATGGGGATTAAAACTTATGATCTTGCCGCCTCACTGACGCTGGTGATGGCCCAGCGCTTAGTCAGAAAACTCT
GCGCGTATTGCAAAGAGCCAGCAAAACGAAACATCGATGCTAAGGACGATTTACTCGGACCCGTCACTGAACAGCAGTAT
CAAGCCAATCAGCACGGTTGTACTCAATGTAATCAGGGTTATCAAGGCCGAATAGGCTTGTTTGAAGTACTGCCAATAAA
CGCCAAACTGCAACAAGCGATATTGCAATGCGCGACTGCCATTGAGATTGAAACGCTCGCCAAAAATGAAGGCATGATGA
GCTTACAACAATCCGGTATTGCCGCACTCAACGCTGGCAAAACCAGTCGCCAAGAATTACAACGCGTGTTGCTATTTGGC
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-139)

Type II/IV secretion system protein (T2SSE)

(185-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

59.043

100

0.595
  pilB Vibrio cholerae strain A1552

58.437

100

0.588
  pilB Vibrio campbellii strain DS40M4

57.726

100

0.58
  pilB Legionella pneumophila strain ERS1305867

46.328

94.821

0.439
  pilB Acinetobacter baumannii D1279779

44.636

93.214

0.416
  pilB Acinetobacter baylyi ADP1

43.893

93.571

0.411
  pilF Neisseria gonorrhoeae MS11

43.487

93.214

0.405

Multiple sequence alignment