Detailed information NTG0183955

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: A9E78_RS04575
• Genome accession: NZ_CP015933
• Coordinates: 1011807..1012871 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain C2_S
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1006807..1017871

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9E78_RS04565 (A9E78_04565)	-	1007847..1010231 (-)	2385	WP_027220940.1	penicillin-binding protein 1A	-
A9E78_RS04570 (A9E78_04570)	ravI	1010383..1011390 (-)	1008	WP_011946020.1	Dot/Icm T4SS effector RavI	-
A9E78_RS04575 (A9E78_04575)	pilM	1011807..1012871 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
A9E78_RS04580 (A9E78_04580)	pilN	1012875..1013423 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
A9E78_RS04585 (A9E78_04585)	pilO	1013437..1014036 (+)	600	WP_013101267.1	type 4a pilus biogenesis protein PilO	Machinery gene
A9E78_RS04590 (A9E78_04590)	pilP	1014033..1014617 (+)	585	WP_013101268.1	pilus assembly protein PilP	Machinery gene
A9E78_RS04595 (A9E78_04595)	pilQ	1014621..1016717 (+)	2097	WP_011946023.1	type IV pilus secretin PilQ	Machinery gene
A9E78_RS04600 (A9E78_04600)	aroK	1017164..1017691 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=183955 A9E78_RS04575 WP_011215094.1 1011807..1012871(+) (pilM) [Legionella pneumophila strain C2_S]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=183955 A9E78_RS04575 WP_011215094.1 1011807..1012871(+) (pilM) [Legionella pneumophila strain C2_S]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407