Detailed information NTG0183278

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC77_RS10090
• Genome accession: NZ_CP015906
• Coordinates: 1957600..1958247 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain UC77
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1952600..1963247

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC77_RS10065 (LLUC77_1922)	atpF	1952670..1953176 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLUC77_RS10070 (LLUC77_1923)	atpB	1953191..1953904 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLUC77_RS10075 (LLUC77_1924)	-	1953949..1954164 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLUC77_RS10080 (LLUC77_1925)	-	1954348..1955124 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLUC77_RS10085 (LLUC77_1926)	comEC	1955409..1957619 (-)	2211	WP_081172221.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLUC77_RS10090 (LLUC77_1927)	comEA	1957600..1958247 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LLUC77_RS10095 (LLUC77_1928)	-	1958307..1959668 (-)	1362	WP_021214597.1	ABC transporter permease	-
LLUC77_RS10100 (LLUC77_1929)	-	1959665..1960597 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLUC77_RS10105 (LLUC77_1930)	-	1960702..1961100 (-)	399	WP_004255236.1	hypothetical protein	-
LLUC77_RS10110 (LLUC77_1931)	-	1961204..1961767 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLUC77_RS10115 (LLUC77_1932)	-	1961940..1963118 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=183278 LLUC77_RS10090 WP_010906131.1 1957600..1958247(-) (comEA) [Lactococcus lactis subsp. lactis strain UC77]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=183278 LLUC77_RS10090 WP_010906131.1 1957600..1958247(-) (comEA) [Lactococcus lactis subsp. lactis strain UC77]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386