Detailed information NTG0183192

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLUC11_RS10810
• Genome accession: NZ_CP015904
• Coordinates: 2192440..2192886 (-)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: A0AAP4JUI5
• Organism: Lactococcus lactis subsp. lactis strain UC11
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2187440..2197886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC11_RS10775 (LLUC11_2081)	-	2187557..2187934 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLUC11_RS10780 (LLUC11_2082)	-	2188139..2189005 (+)	867	WP_025017140.1	RluA family pseudouridine synthase	-
LLUC11_RS10785 (LLUC11_2083)	-	2189043..2189852 (-)	810	WP_014570791.1	metal ABC transporter permease	-
LLUC11_RS10790 (LLUC11_2084)	-	2189845..2190582 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
LLUC11_RS10795 (LLUC11_2085)	-	2190759..2191601 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
LLUC11_RS10800 (LLUC11_2086)	-	2191598..2192035 (-)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
LLUC11_RS10805 (LLUC11_2087)	comGG	2192117..2192401 (-)	285	WP_025017139.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLUC11_RS10810 (LLUC11_2088)	comGF	2192440..2192886 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLUC11_RS10815 (LLUC11_2089)	comGE	2192849..2193145 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLUC11_RS10820 (LLUC11_2090)	comGD	2193235..2193534 (-)	300	WP_289667678.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
LLUC11_RS10825 (LLUC11_2091)	comGC	2193509..2193892 (-)	384	WP_081040674.1	competence type IV pilus major pilin ComGC	Machinery gene
LLUC11_RS10830 (LLUC11_2092)	comGB	2193906..2194979 (-)	1074	WP_081041295.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLUC11_RS10835 (LLUC11_2093)	comGA	2194873..2195811 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=183192 LLUC11_RS10810 WP_029344525.1 2192440..2192886(-) (comGF) [Lactococcus lactis subsp. lactis strain UC11]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=183192 LLUC11_RS10810 WP_029344525.1 2192440..2192886(-) (comGF) [Lactococcus lactis subsp. lactis strain UC11]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385