Detailed information NTG0183043

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLJM3_RS11770
• Genome accession: NZ_CP015901
• Coordinates: 2261509..2261934 (-)
• Length: 141 a.a.
• NCBI ID: WP_011677182.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain JM3
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2256509..2266934

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLJM3_RS11745 (LLJM3_2403)	-	2258112..2258921 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLJM3_RS11750 (LLJM3_2404)	-	2258914..2259651 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLJM3_RS11755 (LLJM3_2405)	-	2259830..2260672 (-)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLJM3_RS11760 (LLJM3_2406)	-	2260669..2261106 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLJM3_RS11765 (LLJM3_03405)	comGG	2261186..2261485 (-)	300	WP_011677181.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLJM3_RS11770 (LLJM3_2408)	comGF	2261509..2261934 (-)	426	WP_011677182.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLJM3_RS11775 (LLJM3_2409)	comGE	2261918..2262214 (-)	297	WP_011677183.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLJM3_RS11780 (LLJM3_2410)	comGD	2262186..2262374 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLJM3_RS11785 (LLJM3_2411)	comGC	2262576..2262926 (-)	351	WP_050574401.1	competence type IV pilus major pilin ComGC	Machinery gene
LLJM3_RS11790 (LLJM3_2412)	comGB	2262971..2263997 (-)	1027	Protein_2295	competence type IV pilus assembly protein ComGB	-
LLJM3_RS11795 (LLJM3_03410)	-	2263897..2264718 (-)	822	Protein_2296	ATPase, T2SS/T4P/T4SS family	-
LLJM3_RS11800 (LLJM3_2414)	-	2264821..2265711 (+)	891	WP_011677100.1	IS982 family transposase	-
LLJM3_RS11805 (LLJM3_03415)	comGA	2265693..2265884 (-)	192	WP_041167484.1	hypothetical protein	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16194.73 Da
• Isoelectric Point: 8.4549

>NTDB_id=183043 LLJM3_RS11770 WP_011677182.1 2261509..2261934(-) (comGF) [Lactococcus cremoris strain JM3]
MKLKIRAFTLLECLVALLAISGSVPVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=183043 LLJM3_RS11770 WP_011677182.1 2261509..2261934(-) (comGF) [Lactococcus cremoris strain JM3]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCCAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	99.291	100	0.993
comYF	Streptococcus mutans UA140	43.972	100	0.44
comYF	Streptococcus mutans UA159	43.262	100	0.433
comGF/cglF	Streptococcus mitis NCTC 12261	38.571	99.291	0.383
comGF/cglF	Streptococcus mitis SK321	38.571	99.291	0.383
comGF/cglF	Streptococcus pneumoniae Rx1	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae D39	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae R6	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae TIGR4	37.143	99.291	0.369