Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LL275_RS11735 Genome accession   NZ_CP015897
Coordinates   2311420..2312493 (-) Length   357 a.a.
NCBI ID   WP_014570824.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain 275     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2306420..2317493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL275_RS11690 (LL275_2263) - 2306550..2307359 (-) 810 WP_014570791.1 metal ABC transporter permease -
  LL275_RS11695 (LL275_2264) - 2307352..2308089 (-) 738 WP_003129989.1 metal ABC transporter ATP-binding protein -
  LL275_RS11700 (LL275_2265) - 2308266..2309108 (-) 843 WP_003129990.1 metal ABC transporter substrate-binding protein -
  LL275_RS11705 (LL275_2266) - 2309105..2309542 (-) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  LL275_RS11710 (LL275_2267) comGG 2309632..2309916 (-) 285 WP_003129993.1 competence type IV pilus minor pilin ComGG Machinery gene
  LL275_RS11715 (LL275_2268) comGF 2309955..2310401 (-) 447 WP_029344525.1 competence type IV pilus minor pilin ComGF Machinery gene
  LL275_RS11720 (LL275_2269) comGE 2310364..2310660 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  LL275_RS11725 (LL275_2270) comGD 2310632..2311063 (-) 432 WP_080585155.1 competence type IV pilus minor pilin ComGD Machinery gene
  LL275_RS11730 (LL275_2271) comGC 2311023..2311406 (-) 384 WP_081040865.1 competence type IV pilus major pilin ComGC Machinery gene
  LL275_RS11735 (LL275_2272) comGB 2311420..2312493 (-) 1074 WP_014570824.1 competence type IV pilus assembly protein ComGB Machinery gene
  LL275_RS11740 (LL275_2273) comGA 2312387..2313326 (-) 940 Protein_2268 competence type IV pilus ATPase ComGA -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40366.59 Da        Isoelectric Point: 10.4062

>NTDB_id=182901 LL275_RS11735 WP_014570824.1 2311420..2312493(-) (comGB) [Lactococcus lactis subsp. lactis strain 275]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=182901 LL275_RS11735 WP_014570824.1 2311420..2312493(-) (comGB) [Lactococcus lactis subsp. lactis strain 275]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACCTTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.698

95.518

0.79
  comYB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comYB Streptococcus mutans UA140

53.372

95.518

0.51
  comYB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae D39

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae R6

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

93.838

0.482
  comGB/cglB Streptococcus mitis SK321

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.149

93.838

0.471

Multiple sequence alignment