Detailed information NTG0182897

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LL275_RS11715
• Genome accession: NZ_CP015897
• Coordinates: 2309955..2310401 (-)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: A0AAP4JUI5
• Organism: Lactococcus lactis subsp. lactis strain 275
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2304955..2315401

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL275_RS11680 (LL275_2261)	-	2305064..2305441 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LL275_RS11685 (LL275_2262)	-	2305645..2306511 (+)	867	WP_003129984.1	RluA family pseudouridine synthase	-
LL275_RS11690 (LL275_2263)	-	2306550..2307359 (-)	810	WP_014570791.1	metal ABC transporter permease	-
LL275_RS11695 (LL275_2264)	-	2307352..2308089 (-)	738	WP_003129989.1	metal ABC transporter ATP-binding protein	-
LL275_RS11700 (LL275_2265)	-	2308266..2309108 (-)	843	WP_003129990.1	metal ABC transporter substrate-binding protein	-
LL275_RS11705 (LL275_2266)	-	2309105..2309542 (-)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
LL275_RS11710 (LL275_2267)	comGG	2309632..2309916 (-)	285	WP_003129993.1	competence type IV pilus minor pilin ComGG	Machinery gene
LL275_RS11715 (LL275_2268)	comGF	2309955..2310401 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL275_RS11720 (LL275_2269)	comGE	2310364..2310660 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL275_RS11725 (LL275_2270)	comGD	2310632..2311063 (-)	432	WP_080585155.1	competence type IV pilus minor pilin ComGD	Machinery gene
LL275_RS11730 (LL275_2271)	comGC	2311023..2311406 (-)	384	WP_081040865.1	competence type IV pilus major pilin ComGC	Machinery gene
LL275_RS11735 (LL275_2272)	comGB	2311420..2312493 (-)	1074	WP_014570824.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL275_RS11740 (LL275_2273)	comGA	2312387..2313326 (-)	940	Protein_2268	competence type IV pilus ATPase ComGA	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=182897 LL275_RS11715 WP_029344525.1 2309955..2310401(-) (comGF) [Lactococcus lactis subsp. lactis strain 275]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=182897 LL275_RS11715 WP_029344525.1 2309955..2310401(-) (comGF) [Lactococcus lactis subsp. lactis strain 275]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385