Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FORC36_RS13885 Genome accession   NZ_CP015512
Coordinates   2882959..2884749 (-) Length   596 a.a.
NCBI ID   WP_085761369.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_036     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2877959..2889749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC36_RS13865 (FORC36_2583) - 2878661..2879488 (-) 828 WP_017789766.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  FORC36_RS13870 (FORC36_2584) - 2879568..2881064 (-) 1497 WP_011079359.1 AAA family ATPase -
  FORC36_RS13875 (FORC36_2585) aroB 2881115..2882206 (-) 1092 WP_011079358.1 3-dehydroquinate synthase -
  FORC36_RS13880 (FORC36_2586) aroK 2882239..2882757 (-) 519 WP_011079357.1 shikimate kinase AroK -
  FORC36_RS13885 (FORC36_2587) pilQ 2882959..2884749 (-) 1791 WP_085761369.1 type IV pilus secretin PilQ Machinery gene
  FORC36_RS13890 (FORC36_2588) pilP 2884763..2885275 (-) 513 WP_085761370.1 pilus assembly protein PilP Machinery gene
  FORC36_RS13895 (FORC36_2589) pilO 2885265..2885855 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC36_RS13900 (FORC36_2590) pilN 2885848..2886438 (-) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  FORC36_RS13905 (FORC36_2591) pilM 2886428..2887435 (-) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 596 a.a.        Molecular weight: 64664.75 Da        Isoelectric Point: 4.5316

>NTDB_id=180229 FORC36_RS13885 WP_085761369.1 2882959..2884749(-) (pilQ) [Vibrio vulnificus strain FORC_036]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGSGDSGSGGSGSEGGSASID
DFLNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTV
SFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKV
PLLGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1791 bp        

>NTDB_id=180229 FORC36_RS13885 WP_085761369.1 2882959..2884749(-) (pilQ) [Vibrio vulnificus strain FORC_036]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACCTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACGGTGGTGGATTTGAAACCGGTAGCGGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTGTTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGTATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTACGTAATGTTTTGCAGCTGATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTCTAGATAAACGTGTTGAAGGTAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTGAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCGCGAATTGTCACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTACGTTG
GGGTTTTACCTCCATCAACGGCAGCACAACTGTCGGTGGTTCAATCGAAAACAACCTGGCAACCATTGGTCTCTACAATG
CAGGCAGTGGTAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTAGTGAAGGCGGTTCGGCCTCCATTGAT
GATTTCCTCAATGTCAATCTTGCTGCGACCTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGA
TTTGTTGTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAA
CCAATAAGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTT
TCATTTAAGAAAGCCGTGCTTAGCCTGAAAGTGACCCCACAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGT
TACACAAGATAGACCCGGCGAAATTGTCAAAACAGGTACTGGAGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACAC
AAGTCTTGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATTACCAATACCACGGACAAGGTT
CCTCTGTTAGGCGATTTACCTTTATTGGGGGCGCTTTTCCGCCGTAGCTATGAAAACGTGGGTAAACGTGAATTGCTGAT
ATTTGTCACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Secretin and TonB N terminus short domain (STN)

(186-234)

Bacterial type II and III secretion system protein (Secretin)

(436-594)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.534

99.329

0.77
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.48

99.329

0.73
  pilQ Vibrio cholerae strain A1552

73.48

99.329

0.73
  pilQ Legionella pneumophila strain ERS1305867

41.012

96.141

0.394
  pilQ Legionella pneumophila strain Lp02

41.281

94.295

0.389
  pilQ Pseudomonas aeruginosa PAK

39.683

95.134

0.378
  comQ Acinetobacter baylyi ADP1

37.037

99.664

0.369

Multiple sequence alignment