Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   S4054249_RS03165 Genome accession   NZ_CP015411
Coordinates   762734..764410 (-) Length   558 a.a.
NCBI ID   WP_046356878.1    Uniprot ID   A0A0F6A935
Organism   Pseudoalteromonas luteoviolacea strain S4054249     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 757734..769410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S4054249_RS03145 (S4054249_03145) - 759377..760249 (-) 873 WP_046356882.1 AraC family transcriptional regulator -
  S4054249_RS03150 (S4054249_03150) - 760385..761437 (-) 1053 WP_046356881.1 alpha/beta hydrolase -
  S4054249_RS03155 (S4054249_03155) - 761541..762425 (+) 885 WP_046356880.1 LysR family transcriptional regulator -
  S4054249_RS03160 (S4054249_03160) yacG 762418..762657 (-) 240 WP_046356879.1 DNA gyrase inhibitor YacG -
  S4054249_RS03165 (S4054249_03165) pilB 762734..764410 (-) 1677 WP_046356878.1 type IV-A pilus assembly ATPase PilB Machinery gene
  S4054249_RS03170 (S4054249_03170) coaE 764530..765135 (-) 606 WP_046356877.1 dephospho-CoA kinase -
  S4054249_RS03175 (S4054249_03175) pilD 765138..766028 (-) 891 WP_046356876.1 A24 family peptidase Machinery gene
  S4054249_RS03180 (S4054249_03180) pilC 766064..767299 (-) 1236 WP_046356875.1 type II secretion system F family protein Machinery gene
  S4054249_RS03185 (S4054249_03185) - 767358..767828 (-) 471 WP_046356874.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  S4054249_RS03190 (S4054249_03190) nadC 768083..768922 (-) 840 WP_046356873.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62399.67 Da        Isoelectric Point: 5.4609

>NTDB_id=179289 S4054249_RS03165 WP_046356878.1 762734..764410(-) (pilB) [Pseudoalteromonas luteoviolacea strain S4054249]
MEHHSPLLRKFITLGRVTPDQIKAKQTEATTTAELICLCSGMSGQELAEQCLDLFRVPYFDLNGFDVENVPKDLIKEKLI
RQHYLLPLVKKDRKLFIATSDPTDYGAFENFEFSTGLQCEIVVVDHKLLEQKIDQLFDSSSGLSLSEDEFKEFASLDVED
EPQTQQQDEEKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGILHEMASPPNTLASRLSARIKVMSHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSAAKLGIDVLGYEPEQKDLYLNALEQPQGMILVTGPT
GSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINTKADMTFANALKAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVMSTLHTNSAPETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKEPEELPKEELLKQGFTEEQVKDLTL
YSPKGCDHCTDGYKGRVGVYEVVKITPEMSHKIMEGANSLEIAALADKLGFDNLRKSGLKKAAAGVTSLAEINRVTNY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=179289 S4054249_RS03165 WP_046356878.1 762734..764410(-) (pilB) [Pseudoalteromonas luteoviolacea strain S4054249]
ATGGAACATCACTCTCCGTTACTGAGAAAATTCATTACCTTAGGTCGGGTTACGCCGGATCAGATAAAGGCTAAGCAAAC
CGAAGCAACGACCACGGCTGAATTGATATGCCTGTGTAGTGGCATGTCTGGCCAAGAGCTTGCCGAGCAATGCCTTGATT
TATTTCGAGTACCTTACTTTGATCTGAATGGATTTGATGTTGAAAACGTTCCCAAAGATTTGATCAAAGAAAAGCTGATC
CGACAGCACTACTTATTGCCCCTTGTTAAAAAAGATCGCAAGCTATTTATCGCAACATCCGATCCCACCGATTATGGGGC
ATTCGAAAACTTCGAATTTAGTACCGGTCTACAATGTGAAATCGTAGTTGTCGATCACAAGTTGCTTGAACAAAAAATAG
ATCAGCTATTTGACTCAAGCTCAGGTTTATCCCTCAGCGAAGATGAATTCAAGGAATTTGCCAGCCTCGATGTAGAAGAT
GAACCACAAACACAGCAACAAGATGAAGAAAAAGACGATGCACCTATCATTGTCTACATCAATAAAATCTTGATGGACGC
CATTAAAAAAGGGGCATCAGATTTACACTTTGAGCCCTATGAAAAGAAGTACCGTGTCCGTTTTCGTATTGATGGCATTT
TGCATGAGATGGCCAGCCCTCCTAATACGTTAGCGAGCCGACTATCTGCGCGAATTAAAGTCATGTCTCACCTAGACATC
GCTGAAAAACGTAAACCCCAAGATGGGCGTATCAAGCTCAAAATATCAGAGCGAAAAAGTATCGATTTTCGTGTCAGTAC
ACTCCCGACTATGTGGGGTGAGAAAATCGTAATGCGAATACTCGACTCATCAGCTGCTAAGTTAGGCATCGATGTCTTAG
GTTATGAACCAGAACAAAAAGACTTGTATTTAAATGCGCTTGAGCAACCTCAGGGAATGATTTTAGTTACAGGTCCAACA
GGATCTGGTAAAACGGTTTCACTCTATACAGGTCTTAATATTTTAAATAAACCTGAGCGAAATATCAGTACCGCAGAAGA
TCCTGTTGAAATAAATCTTGAAGGGATCAACCAAGTTCAAATCAATACTAAGGCAGATATGACCTTTGCAAATGCACTTA
AAGCATTTTTACGTCAAGATCCGGATGTGGTGATGGTTGGTGAGATCAGGGACCTCGAAACTGCCGAAATCTCTATCAAA
GCAGCCCAAACTGGTCACTTAGTTATGAGTACTCTGCATACAAATTCAGCACCTGAAACTCTGACACGACTGCTAAATAT
GGGCGTGCCTGCATACAATGTCGCAAGCTCGGTGAGTTTAATTATTGCTCAGCGCCTCGCGCGAAGGTTGTGTCCAAAAT
GCAAAGAACCGGAAGAGTTGCCAAAAGAAGAGCTGCTCAAACAAGGCTTCACAGAAGAACAAGTTAAGGACTTAACGTTG
TACTCCCCTAAAGGCTGTGATCACTGCACGGACGGATATAAAGGCCGTGTCGGTGTATACGAAGTAGTTAAGATCACCCC
TGAGATGTCACATAAAATAATGGAGGGTGCAAACTCTCTGGAGATCGCAGCACTTGCTGATAAGCTTGGCTTTGACAACT
TACGCAAATCAGGTCTCAAAAAAGCAGCCGCGGGAGTCACCTCACTGGCCGAAATTAACCGCGTTACAAATTACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F6A935

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.185

96.774

0.582
  pilB Acinetobacter baylyi ADP1

60.314

91.219

0.55
  pilB Vibrio cholerae strain A1552

53.37

98.387

0.525
  pilB Legionella pneumophila strain ERS1305867

53.005

98.387

0.522
  pilB Vibrio parahaemolyticus RIMD 2210633

54.545

94.624

0.516
  pilB Vibrio campbellii strain DS40M4

53.89

94.444

0.509
  pilF Neisseria gonorrhoeae MS11

48.307

100

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.991

100

0.375
  pilF Thermus thermophilus HB27

36.007

100

0.362

Multiple sequence alignment