Detailed information
Bioinformatically predicted
Overview
| Name | pilU | Type | Machinery gene |
| Locus tag | IEC338SC_RS06550 | Genome accession | NZ_CP015145 |
| Coordinates | 1367878..1368996 (-) | Length | 372 a.a. |
| NCBI ID | WP_063098244.1 | Uniprot ID | A0AB33BL08 |
| Organism | Acinetobacter pittii strain IEC338SC | ||
| Function | mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 1360845..1425763 | 1367878..1368996 | within | 0 |
Gene organization within MGE regions
Location: 1360845..1425763
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IEC338SC_RS06510 (IEC338SC_1306) | argB | 1361788..1362693 (+) | 906 | WP_002121716.1 | acetylglutamate kinase | - |
| IEC338SC_RS06515 (IEC338SC_1307) | - | 1362816..1363598 (+) | 783 | WP_032037403.1 | GNAT family N-acetyltransferase | - |
| IEC338SC_RS06520 (IEC338SC_1308) | - | 1363706..1364554 (+) | 849 | WP_063098241.1 | class II glutamine amidotransferase | - |
| IEC338SC_RS06525 (IEC338SC_1309) | - | 1364806..1365261 (+) | 456 | WP_002121698.1 | bacteriohemerythrin | - |
| IEC338SC_RS06530 (IEC338SC_1310) | - | 1365415..1366482 (+) | 1068 | WP_155726862.1 | hypothetical protein | - |
| IEC338SC_RS06535 (IEC338SC_1311) | - | 1366482..1366796 (+) | 315 | WP_063098243.1 | RnfH family protein | - |
| IEC338SC_RS06540 (IEC338SC_1312) | omlA | 1366861..1367259 (-) | 399 | WP_002121740.1 | outer membrane protein assembly factor BamE | - |
| IEC338SC_RS06545 (IEC338SC_1313) | fur | 1367371..1367808 (+) | 438 | WP_002121758.1 | ferric iron uptake transcriptional regulator | - |
| IEC338SC_RS06550 (IEC338SC_1314) | pilU | 1367878..1368996 (-) | 1119 | WP_063098244.1 | PilT/PilU family type 4a pilus ATPase | Machinery gene |
| IEC338SC_RS06555 (IEC338SC_1315) | pilT | 1369016..1370053 (-) | 1038 | WP_002121749.1 | type IV pilus twitching motility protein PilT | Machinery gene |
| IEC338SC_RS06560 (IEC338SC_1316) | yggS | 1370180..1370866 (+) | 687 | WP_063098245.1 | YggS family pyridoxal phosphate-dependent enzyme | - |
| IEC338SC_RS06565 (IEC338SC_1317) | - | 1371036..1371773 (+) | 738 | WP_063098246.1 | hypothetical protein | - |
| IEC338SC_RS06570 (IEC338SC_1318) | - | 1371939..1372334 (+) | 396 | WP_063098247.1 | hypothetical protein | - |
| IEC338SC_RS06575 (IEC338SC_1319) | sbcC | 1372361..1375957 (-) | 3597 | WP_063098248.1 | AAA family ATPase | - |
| IEC338SC_RS06580 (IEC338SC_1320) | sbcD | 1375967..1377235 (-) | 1269 | WP_063098249.1 | exonuclease SbcCD subunit D | - |
| IEC338SC_RS06585 (IEC338SC_1321) | - | 1377399..1377872 (+) | 474 | WP_063098250.1 | OsmC family protein | - |
| IEC338SC_RS06590 (IEC338SC_1322) | - | 1377934..1378311 (-) | 378 | WP_063098251.1 | VOC family protein | - |
| IEC338SC_RS06595 (IEC338SC_1323) | - | 1378692..1379144 (+) | 453 | WP_017386708.1 | ABZJ_00895 family protein | - |
| IEC338SC_RS06600 (IEC338SC_1324) | yfcH | 1379228..1380133 (+) | 906 | WP_063098252.1 | TIGR01777 family oxidoreductase | - |
| IEC338SC_RS06605 (IEC338SC_1325) | - | 1380125..1381240 (-) | 1116 | WP_063098253.1 | NAD(P)/FAD-dependent oxidoreductase | - |
| IEC338SC_RS06610 (IEC338SC_1326) | hemB | 1381266..1382279 (-) | 1014 | WP_000222569.1 | porphobilinogen synthase | - |
| IEC338SC_RS06615 (IEC338SC_1327) | - | 1382422..1382919 (+) | 498 | WP_002121786.1 | thioesterase family protein | - |
| IEC338SC_RS06620 (IEC338SC_1328) | - | 1383187..1384338 (+) | 1152 | WP_005070833.1 | EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit | - |
| IEC338SC_RS06625 (IEC338SC_1329) | - | 1384345..1385868 (+) | 1524 | WP_063098254.1 | DHA2 family efflux MFS transporter permease subunit | - |
| IEC338SC_RS06630 (IEC338SC_1330) | ggt | 1386173..1388158 (+) | 1986 | WP_063098255.1 | gamma-glutamyltransferase | - |
| IEC338SC_RS06640 (IEC338SC_1332) | - | 1388862..1390004 (+) | 1143 | WP_063098256.1 | tyrosine-type recombinase/integrase | - |
| IEC338SC_RS06645 (IEC338SC_1333) | - | 1390288..1392705 (+) | 2418 | WP_063098257.1 | hypothetical protein | - |
| IEC338SC_RS06650 (IEC338SC_1334) | - | 1393061..1393933 (+) | 873 | WP_063098258.1 | YagK/YfjJ domain-containing protein | - |
| IEC338SC_RS19060 (IEC338SC_1335) | - | 1394033..1394305 (+) | 273 | WP_082188448.1 | hypothetical protein | - |
| IEC338SC_RS06655 (IEC338SC_1336) | - | 1394471..1396285 (+) | 1815 | WP_063098259.1 | DUF4365 domain-containing protein | - |
| IEC338SC_RS06660 (IEC338SC_1337) | - | 1396407..1397141 (-) | 735 | WP_063098260.1 | hypothetical protein | - |
| IEC338SC_RS06665 (IEC338SC_1338) | - | 1397221..1397865 (-) | 645 | WP_063098261.1 | DUF805 domain-containing protein | - |
| IEC338SC_RS06670 (IEC338SC_1339) | - | 1398783..1400123 (+) | 1341 | WP_063098262.1 | hypothetical protein | - |
| IEC338SC_RS06675 (IEC338SC_1340) | - | 1400365..1400550 (+) | 186 | Protein_1302 | hypothetical protein | - |
| IEC338SC_RS06680 (IEC338SC_1341) | - | 1400733..1400963 (+) | 231 | WP_063098264.1 | helix-turn-helix transcriptional regulator | - |
| IEC338SC_RS06685 (IEC338SC_1342) | - | 1401037..1401621 (+) | 585 | WP_063098265.1 | class I SAM-dependent methyltransferase | - |
| IEC338SC_RS06690 (IEC338SC_1343) | - | 1401614..1402621 (+) | 1008 | WP_063098266.1 | HNH endonuclease domain-containing protein | - |
| IEC338SC_RS19070 (IEC338SC_1344) | - | 1402744..1404585 (+) | 1842 | WP_082188450.1 | DUF262 domain-containing protein | - |
| IEC338SC_RS06700 (IEC338SC_1345) | - | 1404688..1407567 (+) | 2880 | WP_063098267.1 | ATP-dependent helicase | - |
| IEC338SC_RS06705 (IEC338SC_1346) | - | 1407568..1409514 (+) | 1947 | WP_063098268.1 | site-specific DNA-methyltransferase | - |
| IEC338SC_RS06710 (IEC338SC_1347) | - | 1409507..1412230 (+) | 2724 | WP_063098269.1 | TnsA endonuclease N-terminal domain-containing protein | - |
| IEC338SC_RS06715 (IEC338SC_1348) | - | 1412478..1414127 (+) | 1650 | WP_146064298.1 | DUF7017 domain-containing protein | - |
| IEC338SC_RS06720 (IEC338SC_1349) | - | 1414161..1416644 (-) | 2484 | WP_063098271.1 | ATP-dependent helicase | - |
| IEC338SC_RS06725 (IEC338SC_1350) | - | 1416646..1418307 (-) | 1662 | WP_063098272.1 | ATP-dependent DNA helicase | - |
| IEC338SC_RS06730 (IEC338SC_1351) | - | 1418337..1418708 (-) | 372 | WP_063098273.1 | DUF4236 domain-containing protein | - |
| IEC338SC_RS06735 | - | 1418873..1419535 (-) | 663 | Protein_1314 | Fic family protein | - |
| IEC338SC_RS06740 (IEC338SC_1353) | cas3f | 1420260..1423508 (+) | 3249 | WP_063098274.1 | type I-F CRISPR-associated helicase Cas3f | - |
| IEC338SC_RS06745 (IEC338SC_1354) | - | 1423755..1424705 (+) | 951 | WP_063098275.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 372 a.a. Molecular weight: 41889.11 Da Isoelectric Point: 6.6403
>NTDB_id=177106 IEC338SC_RS06550 WP_063098244.1 1367878..1368996(-) (pilU) [Acinetobacter pittii strain IEC338SC]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDKQ
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDKQ
Nucleotide
Download Length: 1119 bp
>NTDB_id=177106 IEC338SC_RS06550 WP_063098244.1 1367878..1368996(-) (pilU) [Acinetobacter pittii strain IEC338SC]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACTAAACGCGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTCATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAAACAGTAA
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACTAAACGCGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTCATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAAACAGTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilU | Acinetobacter baylyi ADP1 |
75.397 |
100 |
0.766 |
| pilU | Pseudomonas stutzeri DSM 10701 |
68.464 |
99.731 |
0.683 |
| pilU | Vibrio cholerae strain A1552 |
54.054 |
99.462 |
0.538 |
| pilT | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 |
40.934 |
97.849 |
0.401 |
| pilT | Legionella pneumophila strain Lp02 |
39.941 |
90.86 |
0.363 |
| pilT | Legionella pneumophila strain ERS1305867 |
39.941 |
90.86 |
0.363 |