Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BBX32_RS05835 Genome accession   NZ_CP018677
Coordinates   1197409..1198899 (+) Length   496 a.a.
NCBI ID   WP_031958022.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain LAC4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1192409..1203899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBX32_RS05820 (BBX32_05795) - 1192767..1195574 (+) 2808 WP_000276876.1 DUF2339 domain-containing protein -
  BBX32_RS05825 (BBX32_05800) - 1195564..1196481 (-) 918 WP_000785638.1 mechanosensitive ion channel family protein -
  BBX32_RS05830 (BBX32_05805) - 1196491..1197312 (-) 822 WP_000462728.1 hypothetical protein -
  BBX32_RS05835 (BBX32_05810) pilB 1197409..1198899 (+) 1491 WP_031958022.1 type II secretion system ATPase GspE Machinery gene
  BBX32_RS05840 (BBX32_05815) - 1198954..1199385 (-) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  BBX32_RS05845 (BBX32_05820) - 1199472..1199909 (-) 438 WP_000371729.1 MarR family winged helix-turn-helix transcriptional regulator -
  BBX32_RS05850 (BBX32_05825) - 1200070..1200447 (-) 378 WP_000266123.1 DUF4951 domain-containing protein -
  BBX32_RS05855 (BBX32_05830) - 1200462..1201286 (-) 825 WP_000177091.1 carbon-nitrogen hydrolase family protein -
  BBX32_RS05860 (BBX32_05835) gigB 1201313..1201834 (-) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  BBX32_RS05865 (BBX32_05840) gigA 1201937..1202905 (-) 969 WP_000273589.1 RsbU family protein phosphatase GigA -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55297.16 Da        Isoelectric Point: 6.2037

>NTDB_id=175452 BBX32_RS05835 WP_031958022.1 1197409..1198899(+) (pilB) [Acinetobacter baumannii strain LAC4]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLVANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=175452 BBX32_RS05835 WP_031958022.1 1197409..1198899(+) (pilB) [Acinetobacter baumannii strain LAC4]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTTAATAGTCTATTAAGTACCAGTTATGCAGGGGATACAGGCGAATCTCAACAAGTA
GCTGCGGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
GGATGACGCGCCTATTGTTCGTCTAATCAATGCACTTCTCTCTGAGGCAATTCGTGTCGGAGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGGGTAGATGGTCAATTACGTGAAATTGTGCAGCCACGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCCGAAAAGCGTGTACCGCAAGATGGACGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAGTGGCAAATGACTATGAACGTTTAACTCAG
TTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGATAATACCCGTAACATCTTAACTGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTGGATATGACATTTGCTCGTGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTGGTGATG
GTGGGGGAGATTCGTGATCTGGAAACTGCTGAAATTGCAGTTCAAGCATCTTTAACAGGTCATTTGGTTTTATCGACGCT
ACATACCAACACTGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCTTTCCTGCTTTCAAGCTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCAATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAACCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.598

98.387

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

40.041

97.177

0.389
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilF Neisseria gonorrhoeae MS11

48.477

79.435

0.385
  pilB Acinetobacter baumannii D1279779

47.146

81.25

0.383
  pilB Acinetobacter baylyi ADP1

46.787

78.427

0.367