Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BS103_RS03145 Genome accession   NZ_CP018332
Coordinates   656386..657876 (+) Length   496 a.a.
NCBI ID   WP_001166314.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A1296     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651386..662876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS103_RS03130 (BS103_03115) - 651744..654551 (+) 2808 WP_097355202.1 DUF2339 domain-containing protein -
  BS103_RS03135 (BS103_03120) - 654541..655458 (-) 918 WP_000785636.1 mechanosensitive ion channel family protein -
  BS103_RS03140 (BS103_03125) - 655468..656289 (-) 822 WP_032014496.1 hypothetical protein -
  BS103_RS03145 (BS103_03130) pilB 656386..657876 (+) 1491 WP_001166314.1 type II secretion system ATPase GspE Machinery gene
  BS103_RS03150 (BS103_03135) - 657931..658362 (-) 432 WP_097355203.1 organic hydroperoxide resistance protein -
  BS103_RS03155 (BS103_03140) - 658451..658888 (-) 438 WP_000371731.1 MarR family winged helix-turn-helix transcriptional regulator -
  BS103_RS03160 (BS103_03145) - 659049..659426 (-) 378 WP_000266123.1 DUF4951 domain-containing protein -
  BS103_RS03165 (BS103_03150) - 659441..660265 (-) 825 WP_032014495.1 carbon-nitrogen hydrolase family protein -
  BS103_RS03170 (BS103_03155) gigB 660292..660813 (-) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  BS103_RS03175 (BS103_03160) gigA 660916..661884 (-) 969 WP_000273589.1 RsbU family protein phosphatase GigA -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55296.15 Da        Isoelectric Point: 6.1696

>NTDB_id=175003 BS103_RS03145 WP_001166314.1 656386..657876(+) (pilB) [Acinetobacter baumannii strain A1296]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNSRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWHEADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=175003 BS103_RS03145 WP_001166314.1 656386..657876(+) (pilB) [Acinetobacter baumannii strain A1296]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTCAATAGCCTATTAAGTACCAGTTATGCAGGGGATACAGGCGAATCTCAACAAGTA
GCTGCGGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTGATGGATCAAGA
AGATGACGCGCCTATTGTTCGTCTAATCAATGCACTTCTCTCCGAGGCAATTCGAGTAGGGGCTTCAGATATCCATATTG
AAGCTTTTGAGAAAAAGCTATCGGTTCGTTTACGAGTAGATGGACAATTGCGTGAGATTGTACAACCGCGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCCGAAAAGCGTGTACCGCAAGATGGGCGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCTTCTTCTCATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAGCGTTTAACTCAG
TTGGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACAACGACTTTATATGCTGCCTT
ATCTGATCTAAATGACAATAGCCGTAACATCTTAACTGCTGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTAGATATGACTTTTGCTCGAGCTTTAAAAGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTGGGGGAGATTCGTGATCTAGAAACTGCTGAAATTGCGGTGCAGGCATCTTTAACAGGTCATTTGGTGTTATCAACACT
ACATACCAATACCGCTATTGGTGCAGTGACTCGTCTTAAAGATATGGGGATTGAACCTTTCCTGCTTTCAAGCTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCATGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAATCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilB Legionella pneumophila strain ERS1305867

41.393

98.387

0.407
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB/pilB1 Synechocystis sp. PCC 6803

40.249

97.177

0.391
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilF Neisseria gonorrhoeae MS11

48.731

79.435

0.387
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB Acinetobacter baumannii D1279779

47.395

81.25

0.385
  pilB Vibrio cholerae strain A1552

47.87

80.444

0.385
  pilB Acinetobacter baylyi ADP1

46.787

78.427

0.367