Detailed information NTG0173804

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: WM43_RS14595
• Genome accession: NZ_CP014774
• Coordinates: 3196704..3198410 (-)
• Length: 568 a.a.
• NCBI ID: WP_064339260.1
• Uniprot ID: A0AAC9B949
• Organism: Aeromonas veronii strain AVNIH1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	3198916..3200052	3196704..3198410	flank	506

Gene organization within MGE regions

Location: 3196704..3200052

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WM43_RS14595 (WM43_14590)	pilB	3196704..3198410 (-)	1707	WP_064339260.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
WM43_RS14600 (WM43_14595)	-	3198417..3198860 (-)	444	WP_272936814.1	pilin	-
WM43_RS14605 (WM43_14600)	-	3198916..3200052 (-)	1137	WP_021141311.1	ISAs1-like element ISAs12 family transposase	-

Sequence

Protein

• Length: 568 a.a.
• Molecular weight: 62563.90 Da
• Isoelectric Point: 5.3192

>NTDB_id=173804 WM43_RS14595 WP_064339260.1 3196704..3198410(-) (pilB) [Aeromonas veronii strain AVNIH1]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKTLADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide

• Length: 1707 bp

>NTDB_id=173804 WM43_RS14595 WP_064339260.1 3196704..3198410(-) (pilB) [Aeromonas veronii strain AVNIH1]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCTCAGCGCTA
TCTGAGCCAGGCCAAGGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGACGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAGAGCGTCAATACCGCTGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATACTGATGGATGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTTCGCTTTCGCATCGATGGCATTCTGCATGAAATCGCCACCCCGCCGGTTAACCTCTCCAACCGCTTCTCCGCACGC
CTCAAGGTCATGGCGCGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCGAAACCACAG
GGGATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTAGAGATCAACTTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCCTGCGGCAAGATCCGGATGTAGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTCACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCTGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATATACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATTGCGG
CGATTGCCCAGAAAGAGGGGATGAGAACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGAGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-146)

• Type II/IV secretion system protein (T2SSE) (191-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.965	100	0.562
pilB	Acinetobacter baumannii D1279779	55.986	100	0.56
pilB	Legionella pneumophila strain ERS1305867	56.642	95.423	0.54
pilB	Vibrio cholerae strain A1552	54.758	98.063	0.537
pilB	Vibrio campbellii strain DS40M4	51.22	100	0.518
pilB	Vibrio parahaemolyticus RIMD 2210633	51.596	99.296	0.512
pilF	Neisseria gonorrhoeae MS11	46.809	99.296	0.465
pilB/pilB1	Synechocystis sp. PCC 6803	39.06	97.359	0.38
pilF	Thermus thermophilus HB27	40.341	92.958	0.375