Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LBM2029_RS03015 Genome accession   NZ_CP014702
Coordinates   654794..656521 (-) Length   575 a.a.
NCBI ID   WP_075463376.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain KACC 10722     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 649794..661521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LBM2029_RS02985 (LBM2029_02985) - 650415..650876 (+) 462 WP_075463372.1 NUDIX domain-containing protein -
  LBM2029_RS02990 (LBM2029_02990) - 650869..651084 (-) 216 WP_013211401.1 DNA gyrase inhibitor YacG -
  LBM2029_RS02995 (LBM2029_02995) zapD 651096..651854 (-) 759 WP_013211402.1 cell division protein ZapD -
  LBM2029_RS03000 (LBM2029_03000) coaE 651972..652586 (-) 615 WP_075463373.1 dephospho-CoA kinase -
  LBM2029_RS03005 (LBM2029_03005) pilD 652600..653481 (-) 882 WP_075463374.1 prepilin peptidase Machinery gene
  LBM2029_RS03010 (LBM2029_03010) - 653484..654749 (-) 1266 WP_075463375.1 type II secretion system F family protein -
  LBM2029_RS03015 (LBM2029_03015) pilF 654794..656521 (-) 1728 WP_075463376.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LBM2029_RS03020 (LBM2029_03020) - 656769..658046 (-) 1278 WP_075463377.1 HlyC/CorC family transporter -
  LBM2029_RS03030 (LBM2029_03030) ispB 658325..659293 (-) 969 WP_082317225.1 octaprenyl diphosphate synthase -
  LBM2029_RS23815 rplU 659572..659883 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  LBM2029_RS03040 (LBM2029_03040) rpmA 659921..660181 (+) 261 WP_004633021.1 50S ribosomal protein L27 -
  LBM2029_RS03045 (LBM2029_03045) obgE 660368..661468 (+) 1101 WP_075463378.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63700.50 Da        Isoelectric Point: 7.4491

>NTDB_id=173466 LBM2029_RS03015 WP_075463376.1 654794..656521(-) (pilF) [Ralstonia solanacearum strain KACC 10722]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDAVPAVLSTNRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPTEIKSLIPKQEATQIEYDPG
AAAAVRRTTTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPRETL
LEAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILSHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=173466 LBM2029_RS03015 WP_075463376.1 654794..656521(-) (pilF) [Ralstonia solanacearum strain KACC 10722]
ATGACTCTCGGACTCGCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTTGAACAAAGCGCGCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGATCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGATGCCGTGCCGGCCGTGCTGTCGACCAACAGGCATTTC
CAGCAATTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCCAAGCC
GATCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAACGAAGCGCCGACCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGC
GCAGCCGCTGCGGTGCGGCGCACCACGACCGACGGCATCGACGATGCGCCGGTGGTGCGTTTCCTGCAAAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGTATCGACG
GCATCCTCGCGCAGGTCGCGCAGCCACCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACGGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCCACCGAGCGGCTCGATATCGACCAGCTAGGCTATGAGCCCGAGCAAAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTCTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAAGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGATGCGCCGACCACGCTCACGCGGCTGATGAACATGGGCGTGGCACCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCATGCAAGAAGCCGGGCCCGCTGCCCCGGGAAACACTG
CTGGAGGCCGGTTTCAAGGAAGCCGACCTGGACGGCTCGTGGCAGCCGTACCATCCAGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCAGCCACG
GCACGGCACTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCTGGCCTGCGGAAAGTGAAA
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.519

93.913

0.503
  pilB Acinetobacter baumannii D1279779

50.973

98.261

0.501
  pilB Acinetobacter baylyi ADP1

49.911

97.913

0.489
  pilB Legionella pneumophila strain ERS1305867

46.41

99.304

0.461
  pilB Vibrio cholerae strain A1552

44.784

96.696

0.433
  pilB Vibrio campbellii strain DS40M4

44.834

94.261

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilF Thermus thermophilus HB27

37.699

98.261

0.37

Multiple sequence alignment