Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   AYJ52_RS03195 Genome accession   NZ_CP014477
Coordinates   677898..678359 (-) Length   153 a.a.
NCBI ID   WP_000993711.1    Uniprot ID   A0A009I3S8
Organism   Acinetobacter pittii strain AP_882     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672898..683359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYJ52_RS03170 (AYJ52_03170) - 673245..673628 (+) 384 WP_017387044.1 RidA family protein -
  AYJ52_RS20595 - 673618..673745 (-) 128 Protein_611 hypothetical protein -
  AYJ52_RS03175 (AYJ52_03175) bfd 673848..674042 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  AYJ52_RS03180 (AYJ52_03180) bfr 674286..674750 (+) 465 WP_017387045.1 heteropolymeric bacterioferritin subunit Bfr -
  AYJ52_RS03185 (AYJ52_03185) - 674794..676422 (-) 1629 WP_017387046.1 PglL family O-oligosaccharyltransferase -
  AYJ52_RS03190 (AYJ52_03190) - 676528..677841 (-) 1314 WP_017387047.1 O-antigen ligase family protein -
  AYJ52_RS03195 (AYJ52_03195) pilE 677898..678359 (-) 462 WP_000993711.1 pilin Machinery gene
  AYJ52_RS03200 (AYJ52_03200) - 678709..679269 (-) 561 WP_017387048.1 TPM domain-containing protein -
  AYJ52_RS03205 (AYJ52_03205) - 679263..680345 (-) 1083 WP_017403270.1 TPM domain-containing protein -
  AYJ52_RS03210 (AYJ52_03210) - 680370..680960 (-) 591 WP_002117090.1 LemA family protein -
  AYJ52_RS03215 (AYJ52_03215) - 681091..681978 (-) 888 WP_014207824.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15590.71 Da        Isoelectric Point: 7.5868

>NTDB_id=171829 AYJ52_RS03195 WP_000993711.1 677898..678359(-) (pilE) [Acinetobacter pittii strain AP_882]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYIARSQVSEAMELASGLKGAVAETFSQDATCPTNGTNGIATATDINGK
YVASVTTAGTAAASGGCTITAKMRATGVSKGIQNGELTLTLSNADKGSNVWSCTSTGSNKIEQKYLPKSCTSS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=171829 AYJ52_RS03195 WP_000993711.1 677898..678359(-) (pilE) [Acinetobacter pittii strain AP_882]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTCATGATCGTAGTTGCCATTATTGGTATTTTGGCAGCAATCGCGAT
TCCTGCGTATCAAGACTATATCGCTCGTTCTCAAGTTAGTGAAGCTATGGAGTTAGCTAGTGGATTGAAGGGAGCTGTTG
CAGAGACTTTTAGTCAAGATGCTACTTGCCCAACAAATGGTACTAATGGAATTGCTACTGCTACTGATATTAATGGTAAA
TATGTTGCTTCTGTAACCACGGCAGGTACCGCTGCTGCCTCAGGTGGATGTACAATTACTGCAAAAATGCGGGCAACTGG
AGTTTCAAAAGGTATTCAAAATGGAGAGCTTACTCTAACTCTTAGTAATGCAGATAAAGGTTCAAATGTGTGGAGTTGTA
CTTCAACTGGTTCAAACAAAATTGAACAAAAGTACTTACCTAAATCATGTACTTCTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I3S8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

47.826

100

0.503
  pilE Neisseria gonorrhoeae MS11

43.21

100

0.458
  pilA2 Legionella pneumophila strain ERS1305867

42.581

100

0.431
  pilA Acinetobacter baumannii strain A118

41.509

100

0.431
  pilA2 Legionella pneumophila str. Paris

43.046

98.693

0.425
  pilA Acinetobacter nosocomialis M2

52.459

79.739

0.418
  comP Acinetobacter baylyi ADP1

42.384

98.693

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.447

99.346

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

39.13

100

0.412
  pilA Pseudomonas aeruginosa PAK

39.744

100

0.405
  pilA/pilA1 Eikenella corrodens VA1

38.217

100

0.392
  pilA Vibrio parahaemolyticus RIMD 2210633

41.791

87.582

0.366

Multiple sequence alignment