Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AYJ52_RS03245 Genome accession   NZ_CP014477
Coordinates   691159..693324 (-) Length   721 a.a.
NCBI ID   WP_017387053.1    Uniprot ID   A0A009I3T9
Organism   Acinetobacter pittii strain AP_882     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 686159..698324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYJ52_RS03230 (AYJ52_03230) - 688581..689450 (-) 870 WP_017387052.1 hypothetical protein -
  AYJ52_RS03235 (AYJ52_03235) aroB 689467..690549 (-) 1083 WP_017403271.1 3-dehydroquinate synthase -
  AYJ52_RS03240 (AYJ52_03240) aroK 690578..691147 (-) 570 WP_002116987.1 shikimate kinase AroK -
  AYJ52_RS03245 (AYJ52_03245) pilQ 691159..693324 (-) 2166 WP_017387053.1 type IV pilus secretin PilQ Machinery gene
  AYJ52_RS03250 (AYJ52_03250) pilP 693387..693914 (-) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  AYJ52_RS03255 (AYJ52_03255) pilO 693925..694677 (-) 753 WP_017387054.1 type 4a pilus biogenesis protein PilO Machinery gene
  AYJ52_RS03260 (AYJ52_03260) pilN 694674..695315 (-) 642 WP_017387055.1 PilN domain-containing protein Machinery gene
  AYJ52_RS03265 (AYJ52_03265) pilM 695315..696373 (-) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 721 a.a.        Molecular weight: 78094.56 Da        Isoelectric Point: 6.3496

>NTDB_id=171830 AYJ52_RS03245 WP_017387053.1 691159..693324(-) (pilQ) [Acinetobacter pittii strain AP_882]
MNHVFRQFSMGAVAIAIMQVASAQVSMTNIVPMQIAGQGTEIRVIFNGLPPQPQAYQLENPSRLILDFNKAQQNLIQSKV
AIGTNEASSVDVSSDDQRSRLTVNLKEAGAFTTRIEGNTFILKINSAQAVAKPVSASAMQTQGVSNIGFQRGSQGEGLIV
IDLLGGNTPVDVQQQGSKVVVKTLGSKIPTHLAKRLNVNDFATPVSTIESYNEKGNGVITIQSTGSYEYMAYQAENKLTI
SLKRPQDKNVTSLYKTPNYSGNKLSLDFQDIEVRRVLQLLADFTGINMVAADSVQGNITLRLKDVPWDQALDIILKTKNL
DKRRNGNVIWIAPVAELIKAEEEEAKAVAQSVKLAPLQTEYIQLKYAQAQDIMSLITQGSNNSNGMQRTSDGGNSTSTNL
NTGVDSLGNNVGSLLSPRGTITQDDRTNTLIINDTAQSIDQIRKMIDLLDVQVKQVMVEARIVRASTSFTKELGVKWGIL
SQGITNNNNLLVGGSETTLWNLREPKKDETTGGYKYTIERPDNLNVDLGVTNPAGSIAFGLISMSDFMLDLELSALQADG
YGEVISTPKVMTADKQPAKVATGQQVPYQTTTNSAAGATASTSFKDALLSLDVTPSITPDGKIQMKLDISKDSVSGAAPN
GELILNKNNIKTNVLVNNGETVILGGVFEQTTLNSQTKVPFFGDLPGIGRLFRKDQKSDDKQELLIFVTPRIVNDTLVRN
H

Nucleotide


Download         Length: 2166 bp        

>NTDB_id=171830 AYJ52_RS03245 WP_017387053.1 691159..693324(-) (pilQ) [Acinetobacter pittii strain AP_882]
ATGAATCACGTATTCCGTCAGTTTTCTATGGGAGCTGTGGCGATTGCAATTATGCAGGTTGCATCTGCACAGGTCAGCAT
GACTAATATTGTACCGATGCAAATAGCGGGTCAGGGAACAGAAATTCGAGTGATATTTAATGGCTTGCCACCGCAACCAC
AGGCCTATCAGCTTGAGAATCCTTCACGTTTGATTTTAGATTTTAATAAAGCGCAGCAGAATTTAATACAGTCAAAAGTA
GCGATTGGAACGAATGAAGCGAGTTCGGTCGATGTAAGCTCCGATGATCAGCGTTCACGTTTAACTGTAAATTTGAAAGA
GGCAGGTGCTTTTACTACTCGTATTGAAGGCAACACTTTTATTTTAAAGATTAATTCTGCGCAAGCTGTAGCTAAACCTG
TATCAGCAAGTGCAATGCAAACCCAAGGTGTTTCAAACATCGGCTTCCAACGCGGTAGTCAAGGGGAAGGCCTTATCGTT
ATTGACCTGTTGGGAGGTAATACCCCTGTTGATGTGCAACAACAAGGCAGCAAAGTTGTAGTAAAAACTTTGGGTTCTAA
AATTCCTACACATCTAGCTAAACGCTTAAATGTAAATGATTTTGCGACACCTGTTTCGACAATTGAATCTTATAATGAAA
AAGGCAATGGCGTTATTACGATTCAATCTACTGGTAGCTATGAATATATGGCTTATCAGGCAGAAAATAAATTAACCATT
AGCCTCAAGCGTCCGCAGGACAAAAATGTAACCTCTTTATATAAGACGCCTAATTACTCTGGTAATAAGCTTTCTCTCGA
TTTTCAAGATATTGAAGTTCGTCGCGTTTTACAGTTACTTGCTGATTTTACTGGTATTAATATGGTCGCGGCTGATAGTG
TGCAAGGTAATATCACTTTGCGTTTAAAAGATGTACCATGGGACCAAGCACTTGATATTATTTTAAAAACTAAAAATTTA
GATAAACGCAGAAATGGAAATGTAATTTGGATTGCTCCAGTTGCGGAACTGATCAAGGCTGAAGAAGAAGAAGCAAAAGC
TGTTGCACAGAGCGTGAAATTAGCCCCATTACAAACTGAGTATATTCAACTTAAATATGCGCAAGCTCAGGATATTATGA
GTTTAATTACTCAAGGCTCAAATAATAGCAATGGTATGCAGCGCACATCTGATGGTGGAAATTCTACATCTACTAATTTA
AATACTGGTGTTGATAGTTTAGGTAATAATGTAGGAAGTTTATTGAGTCCCCGTGGAACAATTACTCAAGATGATCGTAC
CAATACCTTAATTATTAATGATACGGCTCAGTCGATTGATCAAATTCGTAAAATGATTGATCTGTTGGATGTACAAGTTA
AACAGGTTATGGTTGAGGCGCGAATTGTAAGAGCATCTACTTCTTTTACCAAAGAGCTCGGTGTTAAATGGGGTATATTA
TCGCAAGGGATCACTAACAATAATAACTTGTTAGTTGGTGGTAGTGAAACGACCTTGTGGAATTTACGAGAGCCCAAAAA
GGATGAGACTACGGGTGGTTATAAATACACGATTGAACGTCCAGATAACTTAAATGTCGATTTAGGTGTAACCAATCCGG
CTGGGAGTATTGCCTTTGGCTTGATTAGTATGTCTGATTTTATGCTTGATCTTGAACTTTCTGCATTACAAGCTGATGGC
TATGGTGAGGTTATTTCTACCCCTAAAGTAATGACTGCAGATAAACAGCCTGCAAAAGTTGCAACAGGTCAACAGGTCCC
ATATCAAACTACAACTAATTCTGCTGCTGGTGCTACAGCAAGTACCTCTTTTAAAGATGCACTATTAAGCCTAGATGTTA
CCCCGAGCATTACGCCTGATGGTAAAATTCAAATGAAATTGGATATTTCAAAGGATTCGGTTTCGGGTGCGGCTCCTAAT
GGTGAATTGATCCTAAATAAAAATAATATCAAGACAAATGTGTTGGTGAATAATGGTGAGACAGTCATTTTAGGAGGGGT
GTTTGAGCAGACAACCCTTAACTCTCAAACCAAAGTTCCTTTCTTTGGGGATTTACCGGGAATTGGACGCCTATTTAGGA
AAGATCAGAAATCTGATGATAAGCAAGAGTTGCTGATTTTCGTTACGCCACGAATTGTTAATGACACTTTGGTGAGAAAT
CATTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(360-455)

Bacterial type II and III secretion system protein (Secretin)

(555-713)

AMIN domain (AMIN)

(40-118)

Secretin and TonB N terminus short domain (STN)

(291-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I3T9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

91.956

100

0.92
  pilQ Acinetobacter baumannii strain A118

91.817

100

0.918
  comQ Acinetobacter baylyi ADP1

74.827

100

0.75
  pilQ Pseudomonas aeruginosa PAK

39.86

99.168

0.395

Multiple sequence alignment