Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AXG94_RS17315 Genome accession   NZ_CP014262
Coordinates   3983243..3985315 (+) Length   690 a.a.
NCBI ID   WP_055134867.1    Uniprot ID   -
Organism   Pseudomonas corrugata strain RM1-1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3978243..3990315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXG94_RS17295 (AXG94_17270) comM 3980455..3981519 (+) 1065 WP_024779909.1 pilus assembly protein PilM Machinery gene
  AXG94_RS17300 (AXG94_17275) - 3981519..3982085 (+) 567 WP_024779908.1 PilN domain-containing protein -
  AXG94_RS17305 (AXG94_17280) pilO 3982082..3982705 (+) 624 WP_055134868.1 type 4a pilus biogenesis protein PilO -
  AXG94_RS17310 (AXG94_17285) - 3982702..3983229 (+) 528 WP_024779906.1 pilus assembly protein PilP -
  AXG94_RS17315 (AXG94_17290) pilQ 3983243..3985315 (+) 2073 WP_055134867.1 type IV pilus secretin PilQ Machinery gene
  AXG94_RS17320 (AXG94_17295) aroK 3985320..3985838 (+) 519 WP_024779904.1 shikimate kinase AroK -
  AXG94_RS17325 (AXG94_17300) aroB 3985987..3987087 (+) 1101 WP_055134866.1 3-dehydroquinate synthase -
  AXG94_RS17330 (AXG94_17305) - 3987098..3988663 (+) 1566 WP_024779902.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74739.98 Da        Isoelectric Point: 5.2014

>NTDB_id=169947 AXG94_RS17315 WP_055134867.1 3983243..3985315(+) (pilQ) [Pseudomonas corrugata strain RM1-1-4]
MNRIFTALGVSLWIALMSPMVLGASLKTLDVAALPGDRVELKLTFDGPPPAPHGYTTEQPARIALDLPGVTNQLPRKTQD
LGSGNARSATVVEAGERTRLIIGLTQLVPYNTRIEGNNLFVVVGKGAEPGTAAQVPRPAAAGVMPARSKAPAGRAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEPGGTFD
YSTYQTDNKLTVSVRPMTVEDLRKRDTDRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNQGNWNSSGVTNGS
STTIGTPGSTSTNQPFVDMGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDLVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=169947 AXG94_RS17315 WP_055134867.1 3983243..3985315(+) (pilQ) [Pseudomonas corrugata strain RM1-1-4]
ATGAACAGGATTTTTACTGCCCTCGGTGTTTCGCTATGGATAGCGCTGATGTCACCGATGGTTCTTGGGGCAAGCCTGAA
GACGCTGGATGTCGCGGCTTTGCCAGGGGATCGTGTCGAGTTGAAGCTGACTTTCGACGGGCCACCACCCGCCCCCCATG
GCTACACCACCGAACAACCGGCGCGCATTGCCCTGGACCTGCCTGGGGTGACCAACCAGCTGCCGCGCAAGACCCAGGAC
CTTGGCAGCGGCAATGCGCGCAGCGCCACGGTGGTCGAGGCGGGGGAGCGCACCCGGCTGATCATCGGTCTCACTCAGTT
GGTGCCGTATAACACTCGGATCGAGGGTAATAATCTGTTCGTGGTAGTCGGCAAGGGCGCGGAGCCCGGGACTGCCGCGC
AAGTCCCGCGTCCGGCGGCTGCCGGCGTGATGCCTGCCAGAAGCAAGGCGCCGGCGGGCCGGGCCATTCGTGGCGTGGAT
TTCCAGCGCGGGACCCAGGGCGAAGGCAATGTAGTGATCGACCTGTCGGACCCTTCCATCGCGCCGGACATCCAGGAGCG
GGAAGGCAAGATCATCCTCAACTTCGCCAGGACCCAATTGCCTGAACCACTGCGGGTACGCCTGGACGTCAAGGACTTCG
CCACCCCGGTGCAGTTCGTCAATGCCAGTGCCGGCTCCGACCGGGCCACTATCAGCATCGAGCCTGGCGGTACCTTCGAT
TACTCCACCTACCAGACCGACAACAAACTGACCGTCAGTGTCCGTCCGATGACAGTCGAGGACTTGCGCAAGCGCGACAC
CGACCGTGCCGCCTACAGCGGTGAAAAGCTGTCCCTGAACTTCCAGGACATCGAAGTGCGCTCGGTCCTGCAACTGATTG
CCGATTTCACCAACCTGAACCTGGTGGCAAGCGACACGGTGCAAGGGGGGATCACCTTGCGATTGCAGAATGTTCCCTGG
GACCAGGCGTTGGATCTGGTCCTGAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTAGCCCCTGC
CGATGAGATTGCGGCTCGCGAGCGCCAGGAGTTGGAGTCCCAGAAACAGATTGCCGACCTGGCGCCGCTGCGGCGAGAGC
TGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAATCGGTGACCAGCGCCGAAGCGAAAGCCGAT
GAACGTGGCACGATCACGGTGGATGAGCGAACCAATAACATCATTGCCTACCAGACCCAGGATCGGCTCGACGAGTTGCG
TCGGATCGTGGCCCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCCCGGATTGTCGAGGCCAACGTCGATTACG
ACAAAAGCCTGGGGGTGCGTTGGGGCGGCTCGGTGCAGAACCAGGGCAACTGGAACTCTTCGGGTGTGACCAACGGCAGC
TCCACCACTATCGGCACGCCGGGCAGCACCAGCACCAACCAGCCGTTCGTCGACATGGGCGCGGCAGGCAATACCTCGGG
TATCGGCATTGCGTTCATCACTGACAACGTACTGCTGGATCTGGAGCTGACGGCCATGGAGAAAACCGGCAACGGGGAAA
TCGTCTCCCAGCCCAAGGTGGTGACTTCGGACAAGGAGACCGCGAAAATCCTCAAGGGCACGGAAATTCCCTACCAGGAA
GCCAGTTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAAGCCTCGCTGTCGCTGGAGGTAACCCCGCAGATCACGCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAACCGGACTACCTGAACAAGGTGCAGGAAGTACCACCGATCA
AGAAGAACGAAGTCAACGCGAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACTCAG
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATCTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.587

100

0.752
  comQ Acinetobacter baylyi ADP1

41.245

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.378

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.378

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.872

97.681

0.38
  pilQ Legionella pneumophila strain ERS1305867

38.839

97.391

0.378

Multiple sequence alignment