Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXG94_RS10960 Genome accession   NZ_CP014262
Coordinates   2582636..2584336 (+) Length   566 a.a.
NCBI ID   WP_053192129.1    Uniprot ID   A0A3M3EES8
Organism   Pseudomonas corrugata strain RM1-1-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2577636..2589336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXG94_RS10940 (AXG94_10930) - 2577834..2580767 (+) 2934 WP_055135084.1 DUF748 domain-containing protein -
  AXG94_RS10945 (AXG94_10935) - 2580769..2581065 (+) 297 WP_053192127.1 DUF2845 domain-containing protein -
  AXG94_RS10950 (AXG94_10940) - 2581369..2581722 (-) 354 WP_024779584.1 BON domain-containing protein -
  AXG94_RS10955 (AXG94_10945) - 2581995..2582411 (-) 417 WP_055135086.1 pilin -
  AXG94_RS10960 (AXG94_10950) pilB 2582636..2584336 (+) 1701 WP_053192129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AXG94_RS10965 (AXG94_10955) pilC 2584339..2585556 (+) 1218 WP_024779581.1 type II secretion system F family protein Machinery gene
  AXG94_RS10970 (AXG94_10960) pilD 2585558..2586427 (+) 870 WP_055135083.1 prepilin peptidase Machinery gene
  AXG94_RS10975 (AXG94_10965) coaE 2586438..2587061 (+) 624 WP_055135082.1 dephospho-CoA kinase -
  AXG94_RS10980 (AXG94_10970) yacG 2587058..2587264 (+) 207 WP_024779578.1 DNA gyrase inhibitor YacG -
  AXG94_RS10985 (AXG94_10975) - 2587269..2587484 (-) 216 WP_024779577.1 hypothetical protein -
  AXG94_RS10990 (AXG94_10980) - 2587548..2588234 (-) 687 WP_024779576.1 energy-coupling factor ABC transporter permease -
  AXG94_RS10995 (AXG94_10985) - 2588569..2589195 (+) 627 WP_024779575.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62606.49 Da        Isoelectric Point: 5.2296

>NTDB_id=169931 AXG94_RS10960 WP_053192129.1 2582636..2584336(+) (pilB) [Pseudomonas corrugata strain RM1-1-4]
MNDIALSGLTKQLVLAELITEQSAQQAQQQAQRNRMPLVSYLVQNRLVQSRQVAEIASEHFGVALLDLNSLDKESQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSTTGLEGMGDVDLDG
LDIESDDDRKQDAIAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRVRTDGMLREVARPPTQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYLAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNQCKKATEIPEETLLKEGFPK
ERIGSFTIYEPVGCEQCNSGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=169931 AXG94_RS10960 WP_053192129.1 2582636..2584336(+) (pilB) [Pseudomonas corrugata strain RM1-1-4]
ATGAATGACATCGCCCTCAGCGGTTTGACCAAGCAACTGGTACTGGCCGAGCTGATCACCGAGCAAAGCGCGCAGCAGGC
GCAGCAGCAAGCCCAACGCAACCGCATGCCCCTGGTCAGCTACCTTGTGCAGAACAGATTGGTCCAGAGTCGCCAGGTTG
CTGAAATTGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAAGCCAGCCCACCGGCCTT
GTCAGTGAAAAACTGGTTCGGCAACACCATGCCCTGCCGCTGTGGCGGCGCGGCAACAAGCTGTTCGTGGGTATCTCCGA
CCCCACCAACCACCAGGCCATCAACGACATCCAGTTCAGTACCGGCCTGACCACCGAAGCCATCCTGGTAGAGGACGACA
AACTCAGCGATGCCATCGAGAAGTTTTTCGAATCCAGCACCACCGGCCTGGAAGGCATGGGGGATGTCGACCTCGACGGC
CTGGACATCGAGTCGGACGACGATCGCAAACAGGACGCCATTGCCGGCCAGGACGCTGACGATGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGTGGCTCTTCCGACCTGCACTTCGAACCCTATGAGAAATCCTACCGGG
TGCGGGTACGTACCGACGGCATGTTGCGGGAGGTGGCGAGGCCGCCGACCCAACTGGCCGGCCGCATCGCCGCGCGCCTG
AAGGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAGCCGCAGGACGGCCGGTTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGACTTCCGGGTCAATACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCTTCCAGCG
CGCAAATGGGTATCGATGCCCTGGGCTACGAACCGGATCAGAAAGAGCTCTACCTCGCGGCCCTCAAACAACCCCAAGGG
CTGATCCTGGTCACCGGGCCGACCGGCTCGGGCAAGACTGTCTCGCTCTACACCGGCCTGAATATCCTCAATACCGTGGA
CATCAATATTTCCACCGCCGAAGATCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCCCGCCAGG
GACTGGACTTTGCCCAGGCCCTGCGCTCCTTCCTGCGTCAGGACCCGGACGTGATCATGGTGGGAGAGATCCGCGACCTG
GAAACGGCGGAAATCGCCATCAAGGCAGCCCAGACCGGGCACATGGTGCTCTCCACCCTGCACACCAACAGTGCCGCGGA
AACCCTGATCCGCTTGCAGAACATGGGCATCCCCGGCTTCAACATCGCCACGGCGGTCCACCTGATCATCGCCCAGCGCC
TGGCGCGAAAACTGTGCAACCAATGCAAGAAGGCCACGGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGCTTCCCCAAG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGTGAACAGTGCAACAGTGGCTACAAAGGACGCGTGGGGGT
TTACGAAGTGGTGAAAAATACGCCCGAACTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGGTTCAACGACCTGCGCACTTCGGGGTTACTGAAAGTGATGCAAGGGGTGACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M3EES8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.979

99.647

0.578
  pilB Acinetobacter baumannii D1279779

57.496

100

0.576
  pilB Legionella pneumophila strain ERS1305867

55.026

100

0.551
  pilB Vibrio cholerae strain A1552

50.353

100

0.504
  pilB Vibrio campbellii strain DS40M4

48.319

99.823

0.482
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.002

97.35

0.477

Multiple sequence alignment