Detailed information NTG0169812

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AXF35_RS04815
• Genome accession: NZ_CP014255
• Coordinates: 1033027..1034091 (+)
• Length: 354 a.a.
• NCBI ID: WP_027221264.1
• Uniprot ID: A0AAX2IUM9
• Organism: Legionella pneumophila subsp. pascullei strain F-4185
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1028027..1039091

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXF35_RS04805 (AXF35_11985)	-	1029060..1031444 (-)	2385	WP_027221262.1	penicillin-binding protein 1A	-
AXF35_RS04810 (AXF35_11980)	-	1031595..1032608 (-)	1014	WP_027221263.1	hypothetical protein	-
AXF35_RS04815 (AXF35_11975)	pilM	1033027..1034091 (+)	1065	WP_027221264.1	pilus assembly protein PilM	Machinery gene
AXF35_RS04820 (AXF35_11970)	pilN	1034095..1034643 (+)	549	WP_027221265.1	PilN domain-containing protein	Machinery gene
AXF35_RS04825 (AXF35_11965)	pilO	1034657..1035256 (+)	600	WP_027221266.1	type 4a pilus biogenesis protein PilO	Machinery gene
AXF35_RS04830 (AXF35_11960)	pilP	1035253..1035837 (+)	585	WP_027221267.1	pilus assembly protein PilP	Machinery gene
AXF35_RS04835 (AXF35_11955)	pilQ	1035841..1037937 (+)	2097	WP_027221268.1	type IV pilus secretin PilQ	Machinery gene
AXF35_RS04840 (AXF35_11950)	aroK	1038380..1038907 (+)	528	WP_032828804.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38794.80 Da
• Isoelectric Point: 5.0331

>NTDB_id=169812 AXF35_RS04815 WP_027221264.1 1033027..1034091(+) (pilM) [Legionella pneumophila subsp. pascullei strain F-4185]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEQVCVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDQEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKNSALLDVLIVASRAENVNSRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIIAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSYMSFGKKVNLDAINNDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=169812 AXF35_RS04815 WP_027221264.1 1033027..1034091(+) (pilM) [Legionella pneumophila subsp. pascullei strain F-4185]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATTAGTTCTTCAGCAGTGAAAATTTTGGAAAT
TTCAGGTTCCGGAGAGCAAGTTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCTCTTGAAGGGAATATGA
TCAAGGACGTCGATGTAGTAGCGAATTGTATCAAAAAATTGGTAGAAAGGTTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATTAGCAAAGTAGTGCAAATTAATGAAGGATTGACTGACCAGGAAATGGAAGAGCTTGT
CGTTATTGAAGCGGATAAGTATATTCCTTACCCAATAGACGAAATAAATCTTGATTTTGAAGTGTTAGGTCATTCTGCCA
AGAATTCAGCGCTACTTGATGTTTTAATCGTGGCCTCAAGGGCAGAAAATGTTAATAGTCGTGTTGAAGCAGCTCATAGA
GCTGGCTTGGAGCCTAAAATCGTTGACGTTGAATCTTATGCAGTAGAACGTTCAGTGCAACAAATTGCTCAAGAATTGCC
AGCAGGTGGACAAGACAAAATTATAGCGATAGTAGATATTGGAGCCAATTACACTCATTTATTTGTTTTGCATGGCATGC
GATTAATCTTTTCAAGAGAAGAAAAATTTGGTGGCATGCAGCTTGTTGAAGCTATTGCAGAACATTATCATATGAATTTA
GAGCAGGCTATTGCCCTAAAGAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGCACTTTGCAGTTTTTTTATTCTACCAGTCAACATGGATTTGTAGACCATATTTTATTAG
CTGGAGGAATGGCAAGGCAACCAGGGTTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTAGCAAATCCG
CTATCCTATATGTCTTTTGGTAAAAAAGTGAATTTGGATGCCATTAATAATGACGCGCCTACACTGATGGTGGCTTGTGG
GTTAGCTCTAAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	96.61	100	0.966
comM	Acinetobacter nosocomialis M2	43.22	100	0.432
pilM	Acinetobacter baumannii D1279779	42.938	100	0.429
comM	Acinetobacter baylyi ADP1	40.96	100	0.41