Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AXF35_RS04835 Genome accession   NZ_CP014255
Coordinates   1035841..1037937 (+) Length   698 a.a.
NCBI ID   WP_027221268.1    Uniprot ID   A0AAX2IX32
Organism   Legionella pneumophila subsp. pascullei strain F-4185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030841..1042937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXF35_RS04810 (AXF35_11980) - 1031595..1032608 (-) 1014 WP_027221263.1 hypothetical protein -
  AXF35_RS04815 (AXF35_11975) pilM 1033027..1034091 (+) 1065 WP_027221264.1 pilus assembly protein PilM Machinery gene
  AXF35_RS04820 (AXF35_11970) pilN 1034095..1034643 (+) 549 WP_027221265.1 PilN domain-containing protein Machinery gene
  AXF35_RS04825 (AXF35_11965) pilO 1034657..1035256 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  AXF35_RS04830 (AXF35_11960) pilP 1035253..1035837 (+) 585 WP_027221267.1 pilus assembly protein PilP Machinery gene
  AXF35_RS04835 (AXF35_11955) pilQ 1035841..1037937 (+) 2097 WP_027221268.1 type IV pilus secretin PilQ Machinery gene
  AXF35_RS04840 (AXF35_11950) aroK 1038380..1038907 (+) 528 WP_032828804.1 shikimate kinase AroK -
  AXF35_RS04845 (AXF35_11945) aroB 1038894..1039997 (+) 1104 WP_027221270.1 3-dehydroquinate synthase -
  AXF35_RS04850 (AXF35_11940) - 1040000..1041454 (+) 1455 WP_027221271.1 SPOR domain-containing protein -
  AXF35_RS04855 (AXF35_11935) - 1041563..1041994 (+) 432 WP_027221272.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77594.94 Da        Isoelectric Point: 9.6310

>NTDB_id=169816 AXF35_RS04835 WP_027221268.1 1035841..1037937(+) (pilQ) [Legionella pneumophila subsp. pascullei strain F-4185]
MRKIIAILILISASLSVAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPADQKT
KTIQLGSLHSYNIVAVRDRIRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQIVNAKHEIKRIDFRGI
EKQGGRILIDVSDTGIPIDVTQVGKEIVVNFLNTRIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=169816 AXF35_RS04835 WP_027221268.1 1035841..1037937(+) (pilQ) [Legionella pneumophila subsp. pascullei strain F-4185]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTGTGGCATATTCTAAAGATAACTCTCTCATTTC
AGTAAAAGTATTACCATTGCCTGAAAACAGAGTACGTATTGATTTTCAATTTTCCAAACCCTTAAAGCAACTGCCTGCAA
GCTTTATTACTCAAAAGCCCCCACGTCTGGTATTGGATTTTATTAGCACCGATATGCAAATTCCCGCCGATCAGAAAACT
AAAACCATTCAATTAGGCTCATTACATAGTTATAACATTGTTGCAGTCAGGGATAGGATAAGGGCCATATTGGAATTGGA
TCGTTCAGTGTCCTATTCTGGCAGTACGGCAGGTTCTGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTGTTTG
AGAATAGCAAAGAGGTATTCATAACTAATCAGATTGTGAATGCAAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAAGGTGGTAGAATCTTAATTGATGTTTCTGATACTGGTATTCCGATTGATGTGACTCAAGTTGGAAAAGAAAT
AGTCGTTAATTTCCTGAATACCAGGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTTC
AGATTATCACGATGCAACAGGAAGGCAAAAAAGTTCGTATGACCATCCTCAGTAAAGGGGATTACGGCCATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTTGATGTGTTTCCTTTAACTGCGGAAGAAATTCGCCAGGCCAAATTGAAGAAACAGGT
ATTCACTGGCAAACGCATTTCTTTGAATTTCCAAAATATTAGTATTCGAGCTGTGCTTCAGCTGCTCGCAGATTTTACCG
GAATCAACATGGTTGTCAGCGACAAAGTGCAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGACCAGGCTCTG
GATATTATTTTAACAACACAAGGGTTAGATAAGCGTAGAAAGGGCAATGTCATGTTGATAGATACCAAGGCTTCCATGGA
TAAAATGGAGGAAGAGCAACTCAAAAGTCAACAGACAATTGAAAAGCTCGAGCCTATCCGTTCTGATTTAATTCAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCTTATTATCAGATAGAGGAAAGATAAGT
GTCGATGCTAGAACAAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGAGAACTCATAAAGCAATT
GGATATCCCTGTTAAGCAAGTACTTATTGAGGCGAGGATTGTTGAAGTAACTAAAGATTTTAGTCAAGATATTGGGATAC
GCTGGGGTGTATCAAGGCCAACTCACTTAAGTGGTACACTTGAGGGTGCCAACGAGTTAGCCAGAGGCACAGCACCAGCC
AATGTTGTCCCTTTGGAAAGACGGTTAAACCTTGATTTGGCAGCTGCCCCTTTAACTGGAGCTACACCCGCCTCGGTAGG
TATTGCGCTAGCCAAGCTTAATGATAATATTCTACTTGATCTGGAATTGTCTGCCTTGGAAAGTGAGGGGCGTGCTGAAT
TAATATCCAGCCCGCGCCTGATTACTACGAATCAACAAGCAGCAGTCATCGAGTCTGGTGAAGAGATTCCCTACCAGGAA
GCGACATCAAGTGGAGCGACTGCTGTAGCATTTAAAAAAGCGGTTTTGAGTTTGAAAGTTACCCCGCAAATTACCCCGGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCTCCACAAACATTCCTTGGGGTTCCAGCAATAATAA
CAAAAGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTGCTTGGGGGTATTTACAAGCAAGATAAGAAT
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTACCTGTTGTTGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATTTTTATTACACCAAGAATAATAACGAATGCTTTATCGATTACAACGATAGAAGGACGTGAAA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-680)

AMIN domain (AMIN)

(39-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

98.854

100

0.989
  pilQ Legionella pneumophila strain ERS1305867

98.854

100

0.989
  comQ Acinetobacter baylyi ADP1

37.868

100

0.387
  pilQ Pseudomonas aeruginosa PAK

37.956

98.138

0.372
  pilQ Acinetobacter baumannii strain A118

34.877

100

0.367
  pilQ Acinetobacter baumannii D1279779

34.877

100

0.367

Multiple sequence alignment