Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AL545_RS09840 Genome accession   NZ_CP014045
Coordinates   348042..349058 (+) Length   338 a.a.
NCBI ID   WP_017821733.1    Uniprot ID   A0AAW7A029
Organism   Vibrio alginolyticus strain FDAARGOS_114     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343042..354058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL545_RS09825 (AL545_09830) - 343473..344201 (-) 729 WP_005382781.1 redoxin family protein -
  AL545_RS09830 (AL545_09835) oxyR 344371..345282 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  AL545_RS09835 (AL545_09840) - 345336..347897 (-) 2562 WP_017821734.1 penicillin-binding protein 1A -
  AL545_RS09840 (AL545_09845) pilM 348042..349058 (+) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  AL545_RS09845 (AL545_09850) pilN 349042..349617 (+) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  AL545_RS09850 (AL545_09855) pilO 349610..350203 (+) 594 WP_017633532.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL545_RS09855 (AL545_09860) pilP 350193..350708 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  AL545_RS09860 (AL545_09865) pilQ 350734..352479 (+) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  AL545_RS09865 (AL545_09870) aroK 352667..353185 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37595.95 Da        Isoelectric Point: 7.2224

>NTDB_id=167913 AL545_RS09840 WP_017821733.1 348042..349058(+) (pilM) [Vibrio alginolyticus strain FDAARGOS_114]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIIERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=167913 AL545_RS09840 WP_017821733.1 348042..349058(+) (pilM) [Vibrio alginolyticus strain FDAARGOS_114]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCATAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAGGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5

Multiple sequence alignment