Detailed information NTG0164475

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AVM03_RS06550
• Genome accession: NZ_CP013727
• Coordinates: 1462174..1462788 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain MBE1283
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1457174..1467788

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AVM03_RS06530 (AVM03_06525)	rpsT	1457474..1457740 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AVM03_RS06535 (AVM03_06530)	holA	1457757..1458798 (-)	1042	Protein_1345	DNA polymerase III subunit delta	-
AVM03_RS20070	-	1458838..1458992 (-)	155	Protein_1346	hypothetical protein	-
AVM03_RS19520	-	1459033..1459167 (+)	135	WP_003152870.1	YqzM family protein	-
AVM03_RS06540 (AVM03_06535)	comEC	1459186..1461537 (-)	2352	WP_058906194.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AVM03_RS06545 (AVM03_06540)	-	1461538..1462107 (-)	570	WP_003152868.1	ComE operon protein 2	-
AVM03_RS06550 (AVM03_06545)	comEA	1462174..1462788 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
AVM03_RS06555 (AVM03_06550)	comER	1462847..1463668 (+)	822	WP_014305449.1	late competence protein ComER	-
AVM03_RS06560 (AVM03_06555)	-	1463737..1464474 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
AVM03_RS06565 (AVM03_06560)	rsfS	1464471..1464827 (-)	357	WP_003152864.1	ribosome silencing factor	-
AVM03_RS06570 (AVM03_06565)	yqeK	1464824..1465405 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AVM03_RS06575 (AVM03_06570)	-	1465395..1465964 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
AVM03_RS06580 (AVM03_06575)	yhbY	1465975..1466265 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AVM03_RS06585 (AVM03_06580)	aroE	1466259..1467101 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=164475 AVM03_RS06550 WP_003152867.1 1462174..1462788(-) (comEA) [Bacillus amyloliquefaciens strain MBE1283]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=164475 AVM03_RS06550 WP_003152867.1 1462174..1462788(-) (comEA) [Bacillus amyloliquefaciens strain MBE1283]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363