Detailed information NTG0164075

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: AU077_RS09215
• Genome accession: NZ_CP013688
• Coordinates: 1849825..1850292 (-)
• Length: 155 a.a.
• NCBI ID: WP_014293768.1
• Uniprot ID: A0A380K3J6
• Organism: Streptococcus gallolyticus strain ICDDRB-NRC-S1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1844825..1855292

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AU077_RS09180 (AU077_09180)	-	1845565..1846200 (-)	636	WP_014293774.1	CPBP family intramembrane glutamic endopeptidase	-
AU077_RS09185 (AU077_09185)	-	1846212..1846664 (-)	453	WP_058621960.1	hypothetical protein	-
AU077_RS09190 (AU077_09190)	-	1846677..1846886 (-)	210	WP_014293772.1	hypothetical protein	-
AU077_RS09195 (AU077_09195)	-	1846896..1847096 (-)	201	WP_012961332.1	helix-turn-helix transcriptional regulator	-
AU077_RS09200 (AU077_09200)	-	1847258..1848457 (-)	1200	WP_014293771.1	acetate kinase	-
AU077_RS09205 (AU077_09205)	comYH	1848510..1849466 (-)	957	WP_014293770.1	class I SAM-dependent methyltransferase	Machinery gene
AU077_RS09210 (AU077_09210)	comYG	1849548..1849853 (-)	306	WP_058621961.1	competence type IV pilus minor pilin ComGG	Machinery gene
AU077_RS09215 (AU077_09215)	comYF	1849825..1850292 (-)	468	WP_014293768.1	competence type IV pilus minor pilin ComGF	Machinery gene
AU077_RS09220 (AU077_09220)	comGE	1850246..1850539 (-)	294	WP_044562943.1	competence type IV pilus minor pilin ComGE	-
AU077_RS09225 (AU077_09225)	comYD	1850493..1850924 (-)	432	WP_014293766.1	competence type IV pilus minor pilin ComGD	Machinery gene
AU077_RS09230 (AU077_09230)	comYC	1850908..1851201 (-)	294	WP_039670352.1	competence type IV pilus major pilin ComGC	Machinery gene
AU077_RS09235 (AU077_09235)	comYB	1851201..1852286 (-)	1086	WP_014293764.1	competence type IV pilus assembly protein ComGB	Machinery gene
AU077_RS09240 (AU077_09240)	comYA	1852168..1853109 (-)	942	WP_014293763.1	competence type IV pilus ATPase ComGA	Machinery gene
AU077_RS09245 (AU077_09245)	-	1853219..1853548 (-)	330	WP_014293762.1	DUF1033 family protein	-

Sequence

Protein

• Length: 155 a.a.
• Molecular weight: 17564.08 Da
• Isoelectric Point: 9.2496

>NTDB_id=164075 AU077_RS09215 WP_014293768.1 1849825..1850292(-) (comYF) [Streptococcus gallolyticus strain ICDDRB-NRC-S1]
MFMRGKRKYYTLKKTSLEAFSLIECLVSLLVISGAILVYNGLTQYISANVHYLSENQEENWLLFSQQLRAELANCQLDKV
ENNKLYVTKSSQKLAFGQSKADDFRKTNASGQGYQPMIFGVKSSAISRDGQKVTMTLNLENGLERTFVYTFETAS

Nucleotide

• Length: 468 bp

>NTDB_id=164075 AU077_RS09215 WP_014293768.1 1849825..1850292(-) (comYF) [Streptococcus gallolyticus strain ICDDRB-NRC-S1]
ATGTTTATGAGGGGAAAACGGAAATATTACACGTTAAAAAAGACTAGTTTAGAGGCATTCAGCCTCATAGAATGTTTGGT
TTCTTTATTAGTAATTTCGGGTGCTATTCTTGTTTACAATGGCTTAACGCAATATATTTCTGCAAATGTGCATTATTTGT
CGGAAAATCAAGAGGAAAACTGGCTTTTGTTTTCACAACAGCTGCGTGCGGAGCTTGCCAATTGTCAATTAGATAAGGTT
GAAAATAACAAACTATATGTGACGAAGTCCAGTCAAAAGTTGGCATTTGGACAGTCAAAGGCTGATGATTTTCGTAAAAC
AAACGCATCTGGTCAAGGCTATCAGCCAATGATATTTGGAGTAAAATCTTCTGCTATTTCTAGAGATGGTCAGAAGGTGA
CAATGACATTGAATTTAGAGAATGGTTTGGAGAGGACATTTGTTTACACTTTTGAAACGGCAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (49-144)

3D structure

• AlphaFold DB: A0A380K3J6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	56.835	89.677	0.51
comYF	Streptococcus mutans UA159	56.115	89.677	0.503
comGF/cglF	Streptococcus pneumoniae Rx1	44.286	90.323	0.4
comGF/cglF	Streptococcus pneumoniae D39	44.286	90.323	0.4
comGF/cglF	Streptococcus pneumoniae R6	44.286	90.323	0.4
comGF/cglF	Streptococcus pneumoniae TIGR4	44.286	90.323	0.4
comGF/cglF	Streptococcus mitis NCTC 12261	42.958	91.613	0.394
comGF/cglF	Streptococcus mitis SK321	42.254	91.613	0.387
comGF	Lactococcus lactis subsp. cremoris KW2	43.478	89.032	0.387