Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AOT82_RS08230 Genome accession   NZ_CP012969
Coordinates   1944066..1945781 (+) Length   571 a.a.
NCBI ID   WP_071002606.1    Uniprot ID   A0A1D9D001
Organism   Psychrobacter sp. AntiMn-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1939066..1950781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT82_RS08200 (AOT82_1737) nusA 1939069..1940553 (-) 1485 WP_071002604.1 transcription termination factor NusA -
  AOT82_RS08205 (AOT82_1739) rimP 1940691..1941191 (-) 501 WP_058023609.1 ribosome maturation factor RimP -
  AOT82_RS08220 (AOT82_1741) secG 1942062..1942370 (-) 309 WP_021814138.1 preprotein translocase subunit SecG -
  AOT82_RS08225 (AOT82_1742) tpiA 1942586..1943398 (-) 813 WP_071002605.1 triose-phosphate isomerase -
  AOT82_RS08230 (AOT82_1743) pilB 1944066..1945781 (+) 1716 WP_071002606.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOT82_RS08235 (AOT82_1744) pilC 1946002..1947225 (+) 1224 WP_071002607.1 type II secretion system F family protein Machinery gene
  AOT82_RS08240 (AOT82_1745) pilD 1947233..1948135 (+) 903 WP_071002608.1 A24 family peptidase Machinery gene
  AOT82_RS08245 (AOT82_1746) coaE 1948239..1948925 (+) 687 WP_071002609.1 dephospho-CoA kinase -
  AOT82_RS08250 (AOT82_1747) rlmB 1948888..1949658 (-) 771 WP_071002610.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AOT82_RS08255 (AOT82_1748) - 1949905..1950240 (-) 336 WP_058023616.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62967.20 Da        Isoelectric Point: 4.8905

>NTDB_id=158661 AOT82_RS08230 WP_071002606.1 1944066..1945781(+) (pilB) [Psychrobacter sp. AntiMn-1]
MSITTSKYGGLAQQLISDGVVSEANMKTAQIESQEQKIGLVPYLVDNKLADSYDLAQMLSQAFGDPLFDLDALSTDVIPK
DLVDEKIVRKFNALPLFKRGQRLFVALSDPTRVDAIDAIAFNSRLSIETIVVEENKLKKRIESVYADTMQSFDSFSNSDL
NVDFEDGDEGNDDGETKLSDGVDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSFRVRFRVDGVMEKIANPPVQLANKIA
ARLKVMSQMDISERRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQQEMFLEALHK
PQGMLLITGPTGSGKTVSLYTGINILNTGATNISTAEDPVEINLEGINQVNVNAKVGLTFANALKSFLRQDPDIVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPINIPRQSLLEIG
FTDADLDNPDNVIYEPVGCNECREGYKGRVGIYEVMKVSPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVT
SIQEMMRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=158661 AOT82_RS08230 WP_071002606.1 1944066..1945781(+) (pilB) [Psychrobacter sp. AntiMn-1]
ATGTCTATTACAACCAGTAAATATGGTGGTCTAGCGCAGCAGCTCATCAGTGATGGTGTCGTTAGCGAAGCAAATATGAA
AACGGCACAGATTGAGTCGCAGGAGCAGAAAATCGGATTGGTGCCGTACCTTGTAGACAATAAACTGGCTGATTCTTATG
ACCTTGCTCAAATGTTATCACAAGCCTTTGGCGATCCCTTGTTCGATCTCGATGCGCTCAGCACTGATGTCATTCCAAAG
GACTTAGTCGATGAAAAAATCGTCCGTAAGTTTAATGCATTGCCATTATTCAAGCGCGGCCAACGCTTATTTGTAGCGCT
CAGTGATCCAACTCGTGTTGATGCTATTGATGCTATTGCCTTTAATTCTCGCTTGTCTATTGAAACGATTGTTGTTGAAG
AAAACAAGCTAAAAAAGCGTATCGAAAGCGTTTACGCTGATACGATGCAGAGCTTTGATAGTTTTTCTAATAGTGACTTG
AATGTTGATTTTGAAGATGGTGATGAAGGTAACGATGACGGTGAAACGAAGCTTAGCGATGGGGTCGATGAGGCACCTGT
TGTAAAATTCGTTAATAAAATGTTGGTCGATGCCATTCGCATGGGTGCCTCTGATTTGCATTTTGAACCTTACGAAAAAT
CCTTCCGAGTACGTTTCCGTGTTGATGGCGTGATGGAAAAGATAGCCAATCCGCCAGTCCAACTGGCCAACAAAATCGCA
GCGCGTTTGAAAGTCATGTCACAGATGGACATCTCAGAGCGTAGGGTCCCGCAAGATGGACGTATCAAGCTCAAAATCTC
TAAAACTAAAGCTATTGATTTCCGTGTGAACTCGTTACCGACCTTATTCGGTGAAAAAATCGTTTTGCGTATCCTAGATC
CTTCATCAGCCATGCTTGGTATTGATGCGCTAGGGTATGAACCCGATCAGCAGGAGATGTTTTTAGAAGCGCTGCACAAA
CCGCAAGGTATGCTACTGATTACTGGCCCTACTGGTTCTGGTAAGACAGTCTCTCTTTACACAGGTATCAATATCTTAAA
CACTGGTGCCACCAATATTTCAACCGCCGAAGACCCTGTGGAGATTAACCTTGAAGGTATCAATCAGGTCAATGTCAACG
CTAAGGTCGGCCTAACCTTTGCTAATGCGCTCAAGTCTTTTTTGCGTCAAGATCCTGATATTGTCATGGTTGGTGAGATT
CGTGACTTGGAGACTGCCGAGATCGCTATTAAAGCGGCACAAACAGGACACATGGTGCTTTCAACCTTGCATACTAACTC
AGCACCTGAAACCTTGACGCGTTTGCGTAATATGGGTGTGGCTTCTTTTAATATTGCAACCTCAGTTAACCTAGTCATTG
CCCAGCGTTTGGCACGGCGACTGTGCAAGAACTGTAAAAAACCGATCAATATCCCTCGTCAAAGTTTGCTAGAGATTGGT
TTTACTGATGCAGATTTGGATAATCCAGACAACGTGATTTATGAGCCAGTGGGCTGCAATGAGTGCCGTGAAGGTTATAA
AGGCCGTGTAGGTATTTATGAGGTGATGAAGGTCAGCCCCGACATCTCACGTATCATTATGGAAGATGGCAATGCCATAG
ATATCAAAGATGCGGCGCTCAAAAATGGTTTTCGAGATCTACGCCGTTCTGGGGTTTTAAAAGTTCTGCAAGGTGTCACT
AGTATTCAAGAAATGATGCGCGTTACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9D001

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.717

99.65

0.685
  pilB Acinetobacter baylyi ADP1

65.141

99.475

0.648
  pilB Legionella pneumophila strain ERS1305867

50.433

100

0.51
  pilF Neisseria gonorrhoeae MS11

49.117

99.124

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

49.438

93.52

0.462
  pilB Vibrio campbellii strain DS40M4

49.067

93.87

0.461
  pilB Vibrio cholerae strain A1552

49.621

92.469

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.511

100

0.371

Multiple sequence alignment