Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AOT82_RS08240 Genome accession   NZ_CP012969
Coordinates   1947233..1948135 (+) Length   300 a.a.
NCBI ID   WP_071002608.1    Uniprot ID   -
Organism   Psychrobacter sp. AntiMn-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1942233..1953135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT82_RS08225 (AOT82_1742) tpiA 1942586..1943398 (-) 813 WP_071002605.1 triose-phosphate isomerase -
  AOT82_RS08230 (AOT82_1743) pilB 1944066..1945781 (+) 1716 WP_071002606.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOT82_RS08235 (AOT82_1744) pilC 1946002..1947225 (+) 1224 WP_071002607.1 type II secretion system F family protein Machinery gene
  AOT82_RS08240 (AOT82_1745) pilD 1947233..1948135 (+) 903 WP_071002608.1 A24 family peptidase Machinery gene
  AOT82_RS08245 (AOT82_1746) coaE 1948239..1948925 (+) 687 WP_071002609.1 dephospho-CoA kinase -
  AOT82_RS08250 (AOT82_1747) rlmB 1948888..1949658 (-) 771 WP_071002610.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AOT82_RS08255 (AOT82_1748) - 1949905..1950240 (-) 336 WP_058023616.1 pyrimidine/purine nucleoside phosphorylase -
  AOT82_RS08260 (AOT82_1749) recN 1950960..1952639 (+) 1680 WP_071002611.1 DNA repair protein RecN -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 33101.63 Da        Isoelectric Point: 8.9103

>NTDB_id=158663 AOT82_RS08240 WP_071002608.1 1947233..1948135(+) (pilD) [Psychrobacter sp. AntiMn-1]
MQFIQLLQENIALALVVFGLLGLCVGSFLNVVIHRIPLMMIYGWRQECSQFMSEQADMPREHTLPIANVVATDQPITLSR
PASRCPHCAHKIRWYENIPLISWLALKGRCSNCKAAIGLRYPVVELVTALLSVLVIYKFGVNATGLSALVLVWTLVALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQGWFVSPTQSIWGLLFGFLSLWIVVKVFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGIILMKKQGESRPFAFGPYIAVAGIVALLYGSDIVSWYLGMYTY

Nucleotide


Download         Length: 903 bp        

>NTDB_id=158663 AOT82_RS08240 WP_071002608.1 1947233..1948135(+) (pilD) [Psychrobacter sp. AntiMn-1]
ATGCAATTCATACAGTTACTACAAGAAAATATAGCTCTAGCCTTGGTCGTATTTGGTTTGTTAGGTCTTTGTGTTGGCAG
TTTTTTAAATGTTGTGATTCATCGTATCCCACTTATGATGATTTACGGTTGGCGACAAGAATGTAGCCAATTTATGAGCG
AACAGGCAGATATGCCACGCGAGCATACGTTACCTATAGCGAATGTCGTGGCTACTGACCAACCCATTACTTTGAGTCGA
CCTGCCTCACGCTGCCCCCATTGCGCTCATAAGATCAGATGGTATGAAAACATACCCTTAATCAGCTGGCTGGCACTAAA
AGGGCGCTGCTCAAACTGCAAAGCTGCGATTGGTTTGCGTTATCCTGTAGTTGAGTTAGTAACCGCTTTACTATCGGTGC
TCGTGATCTACAAATTCGGTGTCAATGCAACTGGATTGTCAGCGCTCGTTTTAGTGTGGACACTAGTGGCTCTAACCGGT
ATTGATTTTGATACGCAGCTACTACCCGATCGCCTGACTTTTCCACTAGCAGGTCTAGGATTGGCCGTCAACTCACAAGG
CTGGTTTGTCTCACCAACGCAGTCGATATGGGGTTTACTCTTCGGATTTTTATCACTCTGGATCGTCGTTAAAGTATTCT
ACTTAATCACTAAGAAACATGGGATGGGACAAGGTGATTTTAAATTACTAGCAGTATTAGGTGCTTGGCTCGGGCCCATG
ATGTTGCCATTAATTATTTTATTATCATCCTTGCTTGGGTCGATCGTCGGAATAATCTTGATGAAAAAACAAGGTGAGAG
CAGGCCTTTTGCCTTTGGTCCTTACATTGCTGTCGCAGGCATTGTTGCTTTATTATACGGCTCAGATATCGTCAGCTGGT
ATCTTGGTATGTATACTTACTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(149-258)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

54.514

96

0.523
  pilD Acinetobacter baumannii D1279779

53.979

96.333

0.52
  pilD Vibrio cholerae strain A1552

48.387

93

0.45
  pilD Vibrio campbellii strain DS40M4

47.038

95.667

0.45
  pilD Neisseria gonorrhoeae MS11

41.786

93.333

0.39

Multiple sequence alignment