Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AO353_RS12495 Genome accession   NZ_CP012830
Coordinates   2748570..2750651 (+) Length   693 a.a.
NCBI ID   WP_054595231.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2743570..2755651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS12475 (AO353_12475) comM 2745782..2746846 (+) 1065 WP_054595227.1 pilus assembly protein PilM Machinery gene
  AO353_RS12480 (AO353_12480) - 2746846..2747412 (+) 567 WP_054595228.1 PilN domain-containing protein -
  AO353_RS12485 (AO353_12485) pilO 2747409..2748032 (+) 624 WP_054595229.1 type 4a pilus biogenesis protein PilO -
  AO353_RS12490 (AO353_12490) - 2748029..2748556 (+) 528 WP_054595230.1 pilus assembly protein PilP -
  AO353_RS12495 (AO353_12495) pilQ 2748570..2750651 (+) 2082 WP_054595231.1 type IV pilus secretin PilQ Machinery gene
  AO353_RS12500 (AO353_12500) aroK 2750656..2751174 (+) 519 WP_054595232.1 shikimate kinase AroK -
  AO353_RS12505 (AO353_12505) aroB 2751322..2752422 (+) 1101 WP_054595233.1 3-dehydroquinate synthase -
  AO353_RS12510 (AO353_12510) - 2752433..2754034 (+) 1602 WP_054595234.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75034.38 Da        Isoelectric Point: 6.5842

>NTDB_id=157783 AO353_RS12495 WP_054595231.1 2748570..2750651(+) (pilQ) [Pseudomonas fluorescens strain FW300-N2E3]
MNRIFSTLGFSLWMALLSPMVQAANLKALDVAALPGDRIELKLAFDGPPPAPHGYTTDQPARIALDLPGVSSQLASKTRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYSTRVEGNTLFVVVGQAAAAQKPRSAAPRVASAMPVAAKTYAPVSKAIRGV
DFQRGAQGEGNVVIDLSDPGISPDIQERDGKIILTFAKTQLPERLRVRLDVKDFATPVQFVNASASADKASISIEPSGTY
DYSTYQTDNKLTVSVRPMTVDDLQKRNADRFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESRKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKV
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVRNKGNWNASGVSNG
ANASSTIGTPGSTSTNSPFVDMGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVAFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=157783 AO353_RS12495 WP_054595231.1 2748570..2750651(+) (pilQ) [Pseudomonas fluorescens strain FW300-N2E3]
ATGAACAGGATCTTCTCAACCCTCGGTTTTTCGCTATGGATGGCGCTGCTTTCTCCGATGGTGCAGGCAGCCAATCTCAA
AGCGCTGGATGTCGCCGCGCTGCCGGGTGATCGCATCGAGTTGAAACTGGCTTTCGACGGGCCACCGCCCGCGCCTCATG
GCTATACCACCGATCAGCCGGCACGCATCGCCCTTGATCTGCCGGGTGTGAGTAGCCAACTGGCGAGCAAGACGCGGGAT
CTGGGAGGCGGCAACGCTCGCAGTGCGACAGTGGTCGAGGCCAAGGACCGCACCCGTTTGATCATCAACCTGACGCAGTT
GACGCCTTACAGCACGCGAGTCGAGGGCAATACGCTGTTCGTGGTGGTTGGGCAGGCTGCTGCCGCTCAGAAACCCCGAT
CCGCGGCACCGCGAGTGGCGAGCGCCATGCCAGTGGCCGCCAAGACTTATGCTCCCGTGAGCAAGGCCATTCGCGGCGTG
GACTTTCAGCGCGGTGCGCAGGGTGAAGGCAATGTCGTCATTGATTTATCAGACCCCGGTATCAGCCCGGACATCCAGGA
GCGTGACGGCAAGATCATTCTCACCTTCGCCAAAACCCAGTTGCCCGAACGGTTGCGGGTTCGCCTGGACGTCAAGGATT
TCGCCACGCCGGTGCAGTTCGTCAACGCCAGTGCGTCTGCCGATAAAGCGAGCATCAGCATCGAGCCCAGTGGTACATAC
GACTATTCGACCTACCAGACCGACAACAAGCTGACCGTCAGTGTCCGCCCGATGACTGTCGACGATCTGCAGAAGCGCAA
CGCAGATCGTTTCGCCTACAGCGGTGAGAAGCTGTCGTTGAATTTTCAGGACATCGATGTGCGCTCGGTACTGCAACTGA
TCGCTGACTTTACCAACCTCAACCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGCCTGCAAAACGTGCCG
TGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGGTTGGACAAGCGCAAGATCGGTAACGTCTTGCTGGTAGCGCC
GGCAGATGAGATTGCCGCCCGCGAGCGCCAGGAGCTGGAGTCGCGCAAACAGATCGCCGAGCTGGCGCCACTGCGCCGTG
AGCTGTTGCAGGTCAACTACGCCAAGGCGGCGGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCGAAAGTC
GACGAGCGTGGTTCGATCACTGTCGACGAGCGAACCAACAATATCATCGCCTACCAGACCCAGGATCGCCTGGATGAGCT
ACGGCGAATCGTCGCTCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGGATCGTCGAAGCCAACGTCGACT
ACGACAAAAGTCTCGGCGTGCGTTGGGGCGGCTCGGTCCGCAACAAGGGTAACTGGAATGCCTCCGGGGTCAGCAACGGG
GCCAACGCCTCATCGACCATTGGTACGCCGGGCAGCACCAGTACCAACTCGCCGTTCGTCGATATGGGCGCGGCAGGGAA
TACATCGGGGATCGGCATCGCCTTCATCACCGATAACGTGTTGCTGGACCTTGAGCTGACGGCCATGGAGAAGACCGGTA
ACGGGGAAATCGTCTCTCAACCCAAAGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGTACGGAAATCCCC
TATCAGGAAGCCAGCTCCAGCGGCGCGACATCGGTGGCGTTCAAGGAGGCCTCGCTGTCTCTGGAGGTAACGCCACAGAT
CACCCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAGAACGAGGTCAATGCCAAAGTACTGGTCAACGATGGCGAAACCATCGTCATTGGTGGGGTTTTCTCA
AATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGT
GGTGTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.566

100

0.759
  comQ Acinetobacter baylyi ADP1

40.329

100

0.424
  pilQ Acinetobacter baumannii strain A118

37.981

100

0.413
  pilQ Acinetobacter baumannii D1279779

37.981

100

0.413
  pilQ Legionella pneumophila strain ERS1305867

38.609

97.547

0.377
  pilQ Legionella pneumophila strain Lp02

38.462

97.547

0.375

Multiple sequence alignment