Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   A9F01_RS14430 Genome accession   NZ_CP015930
Coordinates   3252874..3255051 (+) Length   725 a.a.
NCBI ID   WP_010948552.1    Uniprot ID   Q5ZRL5
Organism   Legionella pneumophila strain Philadelphia_3     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3247874..3260051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9F01_RS14410 (A9F01_14410) lgt2 3248296..3250206 (-) 1911 WP_010948548.1 Dot/Icm type IV secretion system effector glucosyltransferase Lgt2/LegC8 -
  A9F01_RS14415 (A9F01_14415) - 3250332..3251087 (-) 756 WP_015443912.1 pteridine reductase -
  A9F01_RS14420 (A9F01_14420) - 3251264..3252376 (+) 1113 WP_015443911.1 SAM-dependent methyltransferase -
  A9F01_RS14425 (A9F01_14425) - 3252379..3252873 (+) 495 WP_010948551.1 6-carboxytetrahydropterin synthase -
  A9F01_RS14430 (A9F01_14430) priA 3252874..3255051 (+) 2178 WP_010948552.1 primosomal protein N' Machinery gene
  A9F01_RS14435 (A9F01_14435) - 3255048..3255440 (+) 393 WP_010948553.1 thioesterase family protein -
  A9F01_RS14440 (A9F01_14440) - 3255557..3255826 (-) 270 WP_016357044.1 hypothetical protein -
  A9F01_RS14445 (A9F01_14445) thyA 3255902..3256696 (-) 795 WP_010948554.1 thymidylate synthase -
  A9F01_RS14450 (A9F01_14450) lgt 3256693..3257463 (-) 771 WP_010948555.1 prolipoprotein diacylglyceryl transferase -
  A9F01_RS14455 (A9F01_14455) - 3257481..3258287 (-) 807 WP_010948556.1 sulfite exporter TauE/SafE family protein -

Sequence

Protein


Download         Length: 725 a.a.        Molecular weight: 81788.11 Da        Isoelectric Point: 9.1746

>NTDB_id=156777 A9F01_RS14430 WP_010948552.1 3252874..3255051(+) (priA) [Legionella pneumophila strain Philadelphia_3]
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Nucleotide


Download         Length: 2178 bp        

>NTDB_id=156777 A9F01_RS14430 WP_010948552.1 3252874..3255051(+) (priA) [Legionella pneumophila strain Philadelphia_3]
ATGAACGATATTTATCAGGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGCGGAAGAGTATGGGTTCCTTTTCGCAATCAAGCCCGAGTGGGCATTGTAATCAATAAAGTTTGTGACCAGCAAA
CGGGCCATTCGTTAAAGAAAATCACTTCAATAATCGATGAGAAACCTCTCGTTCCTGAAGATCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGCTATTATCAGTCACCATTATCTGAAGTGATTCCTTTGGTAATACCAAAAAAATATCGCCTGGGTTTACC
CTGCTCACTACCTACAGAAGATTTTTATCAATTGAATATACCTGTTGAAAAGGCGAAGACACTCATCTCGAAAAAAGCAA
AAAAACAACATGAGTTGATTGATTTTTTAAGCAGGCACCAGAAAGGGATTTCCAAACAGACCCTAAAACAACATGGCTAC
AACGGAACTCAAATTGCGGCACTACTTGCTTGTCAAGCAATTAACCTGTCACAGCAAGTTACCTTGCCAACCAAAATTCC
TGAAAAACTCAGTCCACCGCTAACACTCAATCCTGAACAAGCCGTTGCCGTAGATTCCATTTTCGAACACATCCATCATT
ACCAATGCTTTTTACTGCAAGGTGTTACCGGGAGTGGTAAGACAGAGGTTTATTTGCATGTCATTGCCAAAATATTGGAG
CAAGGCAAACAAGTATTGGTGCTGGTACCAGAAATCGGGTTAACCCCTCAGCTTTTATCTCGTTTTACAGCCCGATTTAA
CCACCCCATAACCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCCTGGCAGCTGGCAAAAGAGAACAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACCCCTCTACCCAATCTTGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCGCTCAAACAAATGGAAGGGGTGCGTTATTCTGCGCGAGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACAGCCACTCCCAGTCTGGAAACCATCCACAACTACAAACAAAAAAAATATAATTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACACCGTTACACTATCAACTGATTGATTTACGATCGCAAATGGTACAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAACAAAACCAGGTTCTTGTTTTCATTAACCGACGCGGCTT
TGCCCCAGTGTTACTTTGCCATCAATGTGGATGGATGGTTGATTGCAAAGCATGCGATAGCCATTTAACCTTGCATAAAC
AGGCAGGACAAATGATTTGTCATCATTGTGGATTAACCCAAAGAATACCCTTGTTTTGCAATAACTGCCAAAGTAAGGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAGTTTTTAAGCCATCAGTTTCCAGATACGAATGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGACAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTCCCCCGTCTATCCCTGGTCGTTGTCGTAGATGCTGATGCAGGCTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGGCAATTATTAACTCAAGTATCTGGACGAGCAGGGCGAGCAGAACA
TCCCGGGCAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTTAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCGAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCACCTTTTCAGTTTTTAGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGAGCAATTCCTAAAAGCAACCAAAGATCAAATTCAAGCTCATCCTCTCACGGTACTGGGTCC
TGCACCAGCTCCTTTACCACGAAAAGCTAACCAGCATAGGATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTAAGAGAGTGGTTAACAATGAATAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(442-468)

Helicase conserved C-terminal domain (Helicase_C)

(489-585)

Primosomal protein N C-terminal domain (PriA_C)

(629-721)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-99)

Type III restriction enzyme, res subunit (ResIII)

(195-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZRL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

42.219

100

0.43

Multiple sequence alignment