Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A9C00_RS18650 Genome accession   NZ_CP015912
Coordinates   3751810..3753195 (-) Length   461 a.a.
NCBI ID   WP_065304323.1    Uniprot ID   -
Organism   Escherichia coli strain 210205630     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3746810..3758195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9C00_RS18615 (A9C00_18615) mutT 3747256..3747645 (+) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -
  A9C00_RS18625 (A9C00_18625) yacG 3747745..3747942 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  A9C00_RS18630 (A9C00_18630) zapD 3747952..3748695 (-) 744 WP_001194734.1 cell division protein ZapD -
  A9C00_RS18635 (A9C00_18635) coaE 3748695..3749315 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  A9C00_RS31000 - 3749340..3749384 (+) 45 WP_120795372.1 protein YacM -
  A9C00_RS18640 (A9C00_18640) guaC 3749540..3750583 (+) 1044 WP_001217338.1 GMP reductase -
  A9C00_RS18645 (A9C00_18645) hofC 3750618..3751820 (-) 1203 WP_000157236.1 protein transport protein HofC -
  A9C00_RS18650 (A9C00_18650) pilB 3751810..3753195 (-) 1386 WP_065304323.1 type II secretion system protein GspE Machinery gene
  A9C00_RS18655 (A9C00_18655) pilA 3753205..3753645 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  A9C00_RS18665 (A9C00_18665) nadC 3753848..3754741 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A9C00_RS18670 (A9C00_18670) ampD 3754829..3755380 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A9C00_RS18675 (A9C00_18675) ampE 3755377..3756231 (+) 855 WP_000172000.1 beta-lactamase regulator AmpE -
  A9C00_RS18680 (A9C00_18680) aroP 3756274..3757647 (-) 1374 WP_077625509.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50479.23 Da        Isoelectric Point: 6.6241

>NTDB_id=156353 A9C00_RS18650 WP_065304323.1 3751810..3753195(-) (pilB) [Escherichia coli strain 210205630]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLARTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQNPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=156353 A9C00_RS18650 WP_065304323.1 3751810..3753195(-) (pilB) [Escherichia coli strain 210205630]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAGCTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGAATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio campbellii strain DS40M4

47.804

83.948

0.401
  pilB Vibrio cholerae strain A1552

46.212

85.9

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

46.114

83.731

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Acinetobacter baumannii D1279779

43.401

85.466

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Neisseria gonorrhoeae MS11

43.896

83.514

0.367