Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   RN80_RS07625 Genome accession   NZ_CP012646
Coordinates   1556784..1557458 (-) Length   224 a.a.
NCBI ID   WP_060628534.1    Uniprot ID   A0A0M4JM00
Organism   Streptococcus mitis strain KCOM 1350 (= ChDC B183)     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1551784..1562458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN80_RS07600 (RN80_07615) - 1551974..1552903 (-) 930 WP_060628529.1 peptidase U32 family protein -
  RN80_RS07605 (RN80_07620) - 1553030..1553710 (-) 681 WP_060628530.1 ABC transporter ATP-binding protein -
  RN80_RS07610 (RN80_07625) - 1553724..1554779 (-) 1056 WP_060628531.1 ABC transporter permease -
  RN80_RS07615 (RN80_07630) - 1555139..1555426 (+) 288 WP_060628532.1 DUF3270 domain-containing protein -
  RN80_RS07620 (RN80_07635) ciaH 1555460..1556794 (-) 1335 WP_060628533.1 two-component system sensor histidine kinase CiaH Regulator
  RN80_RS07625 (RN80_07640) ciaR 1556784..1557458 (-) 675 WP_060628534.1 two-component system response regulator CiaR Regulator
  RN80_RS07630 (RN80_07645) - 1557567..1560113 (-) 2547 WP_060628535.1 M1 family metallopeptidase -
  RN80_RS07635 (RN80_07650) - 1560235..1560675 (-) 441 Protein_1455 ASCH domain-containing protein -
  RN80_RS07640 (RN80_07655) - 1560635..1561519 (-) 885 WP_060628536.1 putative PEP-binding protein -
  RN80_RS07645 (RN80_07660) - 1561561..1562289 (-) 729 WP_060628537.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 224 a.a.        Molecular weight: 25496.38 Da        Isoelectric Point: 4.3283

>NTDB_id=156034 RN80_RS07625 WP_060628534.1 1556784..1557458(-) (ciaR) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGFQVLKELREKGIMTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYILKDVQ

Nucleotide


Download         Length: 675 bp        

>NTDB_id=156034 RN80_RS07625 WP_060628534.1 1556784..1557458(-) (ciaR) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
ATGATAAAAATCTTATTAGTTGAGGATGACCTAGGGCTGTCAAATTCAGTATTTGACTTTTTAGACGATTTTGCGGATGT
CATGCAGGTATTTGATGGGGAAGAAGGTCTCTACGAAGCTGAGAGTGGCGTCTATGACTTGATTTTGCTTGATTTGATGT
TGCCTGAAAAAAATGGCTTCCAAGTCTTGAAAGAATTGCGTGAAAAGGGGATTATGACTCCAGTTCTGATCATGACTGCC
AAGGAAAGTTTGGATGATAAGGGACATGGATTTGAACTGGGAGCAGATGATTATCTGACCAAACCTTTCTACTTAGAAGA
ACTCAAAATGCGGATTCAAGCCCTTCTCAAACGTTCAGGTAAGTTTAATGAAAATACCTTGACTTATGGAAATATTGTAG
TTAATTTGTCAACAAATACTGTTAAGGTTGAAGATACTCCTGTCGAATTGCTGGGCAAAGAGTTCGATTTACTAGTTTAT
TTCCTTCAAAATCAAAATGTTATTTTGCCTAAGACTCAGATTTTTGATCGTCTATGGGGATTTGATAGTGATACAACGAT
TTCGGTTGTCGAAGTCTATGTTTCAAAAGTCCGTAAGAAATTAAAAGGAACCACTTTTGCAGAGAATTTGCAAACCTTGC
GCAGTGTTGGGTATATTTTAAAAGATGTTCAGTAA

Domains

Predicted by InterproScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-220)

Response regulator receiver domain (Response_reg)

(4-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M4JM00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

99.107

100

0.991
  ciaR Streptococcus pneumoniae D39

99.107

100

0.991
  ciaR Streptococcus pneumoniae R6

99.107

100

0.991
  ciaR Streptococcus pneumoniae TIGR4

99.107

100

0.991
  ciaR Streptococcus mutans UA159

88.393

100

0.884
  covR Lactococcus lactis subsp. lactis strain DGCC12653

36.726

100

0.371
  vicR Streptococcus mutans UA159

35.622

100

0.371

Multiple sequence alignment