Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AM586_RS20085 Genome accession   NZ_CP012640
Coordinates   4489230..4490963 (-) Length   577 a.a.
NCBI ID   WP_052233449.1    Uniprot ID   -
Organism   Massilia sp. WG5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4484230..4495963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM586_RS28920 - 4484971..4485360 (-) 390 WP_307719643.1 SGNH hydrolase domain-containing protein -
  AM586_RS20065 (AM586_05905) - 4485393..4485695 (+) 303 WP_052233452.1 putative quinol monooxygenase -
  AM586_RS20070 (AM586_05910) - 4485846..4486850 (-) 1005 WP_052233451.1 NAD-dependent epimerase/dehydratase family protein -
  AM586_RS20075 (AM586_05915) - 4486985..4487935 (+) 951 WP_082439415.1 LysR family transcriptional regulator -
  AM586_RS20080 (AM586_05920) pilC 4487960..4489195 (-) 1236 WP_052233450.1 type II secretion system F family protein Machinery gene
  AM586_RS20085 (AM586_05925) pilB 4489230..4490963 (-) 1734 WP_052233449.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AM586_RS20090 (AM586_05930) - 4491185..4492483 (-) 1299 WP_052233448.1 HlyC/CorC family transporter -
  AM586_RS20095 (AM586_05935) - 4492549..4493106 (-) 558 WP_052233447.1 ClpP family protease -
  AM586_RS20100 (AM586_05940) xdhA 4493267..4494748 (+) 1482 WP_052233446.1 xanthine dehydrogenase small subunit -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 61923.21 Da        Isoelectric Point: 4.9397

>NTDB_id=155950 AM586_RS20085 WP_052233449.1 4489230..4490963(-) (pilB) [Massilia sp. WG5]
MAAVLPNTTAGTALPGLARALVQSGRLTLPQADGLQKKAVADKAGFIDTLLGAGIIDAQGLAVFCAETFGYPLLDLSTFS
LDSLPANAIDARLMQAQRVVALSKRGNKLSVALSDPTNSQALDQIKFQSEASVEPVIVAHDTLLALLAGISKNAEQSISE
LVGDDAEIEFAEDEQQQATQAEAAAASEVEDAPIVRFLNKVLMDAITLGASDIHFEPYEKQYRIRLRVDGVLRDHLAPPL
SIRDKLVSRIKVLAKLDISEKRVPQDGRMRLVLSPTKSIDFRVSTLPTLFGEKTVMRILDATQAQMGIDSLGYDPDQRAL
LIDAITRPYGMVLVTGPTGSGKTVSLYSCLNVLNKPGINISTAEDPAEINLPGVNQVNVNEKAGLTFPVALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPLDIERD
VLKAAGFRDSDLDGEWKPYGPVGCERCLGSGYKGRVGIYQIMPISPAIEALILANGNSMEIAAQAESEGVNSLRRSGLMK
VKQGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=155950 AM586_RS20085 WP_052233449.1 4489230..4490963(-) (pilB) [Massilia sp. WG5]
ATGGCAGCAGTCCTCCCCAACACGACCGCAGGCACCGCATTGCCCGGGTTGGCGCGCGCCCTGGTCCAGTCCGGGCGACT
GACGCTTCCGCAGGCGGACGGCCTCCAAAAGAAGGCCGTCGCCGACAAGGCTGGTTTCATCGACACCTTGCTCGGCGCCG
GCATCATCGATGCGCAAGGCCTGGCGGTATTCTGCGCCGAGACCTTCGGCTATCCCCTGCTCGACCTCTCCACCTTCAGC
CTGGATTCGCTGCCGGCGAACGCCATCGATGCGCGCCTGATGCAGGCCCAGCGCGTGGTGGCCCTGTCCAAGCGCGGCAA
CAAGCTGTCGGTGGCGCTGTCCGATCCGACCAATTCCCAGGCCCTGGACCAGATCAAGTTCCAGAGCGAAGCCTCGGTCG
AGCCGGTGATCGTCGCCCACGACACACTGCTGGCCCTGCTGGCCGGCATCTCCAAGAACGCCGAACAGAGCATCAGCGAA
CTGGTCGGCGACGATGCCGAGATCGAGTTCGCCGAGGACGAGCAGCAGCAGGCCACGCAGGCCGAAGCGGCGGCCGCCTC
CGAGGTCGAGGACGCGCCGATCGTGCGCTTCCTGAACAAGGTGCTGATGGATGCGATCACCCTCGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAGAAGCAGTACCGGATCCGCCTGCGCGTGGACGGCGTGCTGCGCGACCACCTGGCGCCGCCGCTG
TCGATCCGCGACAAGCTGGTCTCGCGCATCAAGGTGCTGGCCAAGCTCGACATCTCCGAGAAAAGGGTCCCGCAGGATGG
CCGCATGCGCCTGGTGCTGTCGCCGACCAAGTCGATCGACTTCCGCGTGAGCACCCTGCCGACCCTGTTCGGCGAAAAGA
CCGTCATGCGTATCCTCGACGCCACCCAGGCGCAGATGGGCATCGACTCGCTCGGCTACGACCCGGACCAGAGGGCGCTG
CTGATCGACGCCATCACCCGTCCCTACGGCATGGTGCTGGTAACGGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTA
CAGCTGCCTGAACGTGCTGAACAAGCCGGGCATCAATATCTCGACGGCCGAGGATCCGGCCGAGATCAACCTGCCGGGCG
TGAACCAGGTCAACGTCAACGAAAAGGCGGGCCTCACCTTCCCGGTCGCGCTGAAGGCTTTTCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAGATCCGCGACCTGGAAACCGCCGACATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGTT
CTCGACCCTGCACACGAACGACGCGCCCTCGACCCTGACCCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCT
CCTCCGTCATCCTGATCACGGCCCAGCGCCTGGCGCGCCGCCTGTGCACCTGCAAGCAGCCGCTCGACATCGAGCGCGAC
GTGCTGAAGGCGGCCGGCTTCCGCGACAGCGACCTGGACGGCGAATGGAAGCCCTACGGCCCGGTCGGCTGCGAACGATG
CCTGGGTTCGGGCTACAAGGGCCGGGTCGGCATCTACCAGATCATGCCGATCTCGCCGGCGATCGAGGCCCTGATCCTGG
CCAACGGCAACTCGATGGAAATCGCCGCCCAGGCCGAATCCGAGGGCGTGAATTCGCTGCGCCGTTCCGGCCTGATGAAG
GTGAAGCAGGGACTGACCAGCCTCGAGGAAGTGCTCGGCTGCACTAACGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-151)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.054

99.307

0.527
  pilF Neisseria gonorrhoeae MS11

53.439

98.267

0.525
  pilB Acinetobacter baumannii D1279779

53.263

98.267

0.523
  pilB Legionella pneumophila strain ERS1305867

49.554

97.227

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

99.133

0.451
  pilB Vibrio cholerae strain A1552

45.39

97.747

0.444
  pilB Vibrio campbellii strain DS40M4

44.328

99.307

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.068

94.107

0.386
  pilF Thermus thermophilus HB27

38.993

92.894

0.362

Multiple sequence alignment