Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AK825_RS00365 Genome accession   NZ_CP012533
Coordinates   81270..82979 (-) Length   569 a.a.
NCBI ID   WP_068033676.1    Uniprot ID   -
Organism   Psychrobacter sp. P11G5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 76270..87979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AK825_RS00340 (AK825_00340) - 76854..77189 (+) 336 WP_068033666.1 pyrimidine/purine nucleoside phosphorylase -
  AK825_RS00345 (AK825_00345) rlmB 77399..78169 (+) 771 WP_068033668.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AK825_RS00350 (AK825_00350) coaE 78132..78815 (-) 684 WP_068033670.1 dephospho-CoA kinase -
  AK825_RS00355 (AK825_00355) pilD 79027..79926 (-) 900 WP_068033673.1 A24 family peptidase Machinery gene
  AK825_RS00360 (AK825_00360) pilC 79934..81157 (-) 1224 WP_068033674.1 type II secretion system F family protein Machinery gene
  AK825_RS00365 (AK825_00365) pilB 81270..82979 (-) 1710 WP_068033676.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AK825_RS00370 (AK825_00370) tpiA 83424..84251 (+) 828 WP_068033678.1 triose-phosphate isomerase -
  AK825_RS00375 (AK825_00375) secG 84478..84786 (+) 309 WP_068033680.1 preprotein translocase subunit SecG -
  AK825_RS00390 (AK825_00390) rimP 85818..86318 (+) 501 WP_068033682.1 ribosome maturation factor RimP -
  AK825_RS00395 (AK825_00395) nusA 86466..87950 (+) 1485 WP_068033684.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62685.75 Da        Isoelectric Point: 4.9829

>NTDB_id=155007 AK825_RS00365 WP_068033676.1 81270..82979(-) (pilB) [Psychrobacter sp. P11G5]
MSTTTSTFGGLAQQLTNAGIVSEAHMKTAQTESQQQQISLVSYLVDHNMANAYQLALLMSQSFGDPFFDLSVLSNDVIPK
DLVDEKIVRKFNALPIFKRGQRVFVALSDPTRIDAIDAIAFNSRLSVETVVVEEDKLKKRIDSVYADTMQSFGNFADGDL
NVGFEDTEEDDSETTLTDSIDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMEKMANPPVQLAGKIAAR
LKVMSQMDISERRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKVVLRILDPSAAMLGIDALGYEPDQKEMFLEALHKPQ
GMLLITGPTGSGKTVSLYTGINILNTGATNISTAEDPVEINLEGINQVNVNAKVGLTFSNALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKTVLNVPRQSLLEVGFT
DDDLDDPNSTIYEPVGCSECREGYKGRVGIYEVMKVSPDISRIIMEDGNAIDIKDAAVKNGFRDLRRSGILKVLQGVTSI
QEMMRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=155007 AK825_RS00365 WP_068033676.1 81270..82979(-) (pilB) [Psychrobacter sp. P11G5]
GTGTCTACTACAACCAGTACATTCGGTGGTTTAGCACAGCAGCTAACTAATGCAGGTATCGTTAGCGAAGCGCACATGAA
AACAGCACAGACTGAGTCGCAACAACAGCAAATCAGTCTCGTTTCTTATTTAGTAGACCATAATATGGCCAATGCCTATC
AACTTGCCCTGTTGATGTCGCAATCATTTGGCGATCCCTTTTTCGATCTGAGCGTGCTTAGTAACGATGTCATCCCAAAA
GATTTAGTTGATGAAAAGATTGTCCGTAAATTTAATGCCCTGCCTATCTTTAAGCGCGGCCAGCGAGTATTTGTTGCTTT
AAGTGATCCTACCCGTATCGATGCGATTGATGCGATTGCTTTTAATTCGCGTCTATCTGTTGAAACGGTGGTGGTCGAAG
AAGACAAGCTCAAAAAACGTATTGATAGTGTCTATGCAGATACCATGCAGAGTTTTGGTAACTTCGCTGATGGCGACTTA
AATGTGGGCTTTGAAGACACTGAAGAGGATGACAGCGAGACCACTCTTACCGACAGTATTGATGAGGCACCCGTTGTAAA
GTTCGTTAATAAGATGCTAGTTGACGCTATTCGTATGGGCGCATCTGATTTGCATTTTGAGCCATATGAGAAGTCTTATC
GCGTGCGCTTTCGAGTCGACGGGGTAATGGAAAAAATGGCAAACCCGCCTGTGCAATTAGCAGGGAAAATCGCAGCCCGT
CTTAAAGTCATGTCACAAATGGATATCTCAGAGCGCCGTGTACCACAAGATGGACGTATTAAGCTTAAGTTATCTAAAAA
TAAAGCTATTGATTTTCGGGTGAACTCTTTGCCAACGCTATTCGGTGAAAAGGTCGTGCTACGTATTTTAGATCCCTCTG
CGGCGATGCTTGGTATCGACGCTTTGGGTTATGAGCCTGATCAAAAAGAGATGTTTTTGGAAGCGCTTCACAAACCGCAG
GGCATGTTATTAATTACTGGTCCTACAGGCTCTGGTAAAACGGTATCGTTATATACAGGTATCAACATTTTGAACACTGG
AGCGACCAATATATCTACAGCAGAAGATCCTGTTGAGATTAATCTTGAGGGGATCAATCAGGTCAACGTCAACGCTAAAG
TAGGTCTGACCTTTTCCAATGCTTTAAAATCGTTTTTGCGCCAAGATCCAGACATCGTCATGGTTGGTGAGATTCGTGAT
CTTGAGACCGCTGAGATTGCCATTAAGGCAGCACAGACCGGCCATATGGTACTGTCAACTCTCCATACTAACTCTGCTCC
GGAAACTTTGACTCGACTACGTAACATGGGCGTAGCATCTTTTAATATTGCTACCTCAGTCAACTTAGTTATTGCTCAGC
GGCTAGCACGACGTTTATGCAAAAATTGCAAAACTGTGCTTAATGTACCGAGGCAGAGCTTACTTGAGGTTGGCTTTACC
GATGATGATCTCGACGATCCAAATAGCACCATCTACGAGCCAGTCGGTTGTAGCGAGTGCCGTGAAGGCTACAAAGGTCG
TGTGGGTATTTATGAGGTTATGAAAGTCAGCCCAGATATTTCACGCATTATTATGGAAGACGGTAATGCGATCGATATCA
AAGATGCTGCTGTCAAAAATGGCTTTCGTGATTTGCGACGCTCAGGCATTTTAAAAGTATTGCAAGGTGTAACCAGCATA
CAAGAAATGATGCGGGTGACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.43

99.824

0.673
  pilB Acinetobacter baylyi ADP1

64.085

99.824

0.64
  pilB Legionella pneumophila strain ERS1305867

53.903

94.552

0.51
  pilF Neisseria gonorrhoeae MS11

51.521

98.243

0.506
  pilB Vibrio cholerae strain A1552

48.936

99.121

0.485
  pilB Vibrio campbellii strain DS40M4

46.986

99.121

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

48.79

94.376

0.46

Multiple sequence alignment