Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AK823_RS00375 Genome accession   NZ_CP012529
Coordinates   84056..85765 (-) Length   569 a.a.
NCBI ID   WP_068325362.1    Uniprot ID   -
Organism   Psychrobacter sp. P2G3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 79056..90765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AK823_RS00350 (AK823_00350) - 79640..79975 (+) 336 WP_068033666.1 pyrimidine/purine nucleoside phosphorylase -
  AK823_RS00355 (AK823_00355) rlmB 80185..80955 (+) 771 WP_068033668.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AK823_RS00360 (AK823_00360) coaE 80918..81601 (-) 684 WP_068033670.1 dephospho-CoA kinase -
  AK823_RS00365 (AK823_00365) pilD 81813..82712 (-) 900 WP_068325360.1 A24 family peptidase Machinery gene
  AK823_RS00370 (AK823_00370) pilC 82720..83943 (-) 1224 WP_068033674.1 type II secretion system F family protein Machinery gene
  AK823_RS00375 (AK823_00375) pilB 84056..85765 (-) 1710 WP_068325362.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AK823_RS00380 (AK823_00380) tpiA 86212..87039 (+) 828 WP_068325365.1 triose-phosphate isomerase -
  AK823_RS00385 (AK823_00385) secG 87266..87574 (+) 309 WP_068033680.1 preprotein translocase subunit SecG -
  AK823_RS00400 (AK823_00400) rimP 88606..89106 (+) 501 WP_068033682.1 ribosome maturation factor RimP -
  AK823_RS00405 (AK823_00405) nusA 89254..90738 (+) 1485 WP_068033684.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62686.74 Da        Isoelectric Point: 4.9261

>NTDB_id=154987 AK823_RS00375 WP_068325362.1 84056..85765(-) (pilB) [Psychrobacter sp. P2G3]
MSTTTSTFGGLAQQLTNAGIVSEAHMKTAQTESQQQQISLVSYLVDHNMANAYQLALLMSQSFGDPFFDLSVLSNDVIPK
DLVDEKIVRKFNALPIFKRGQRVFVALSDPTRIDAIDAIAFNSRLSVETVVVEEDKLKKRIDSVYADTMQSFGNFADGDL
NVGFEDTEEDDSETTLTDSIDEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMEKMANPPVQLAGKIAAR
LKVMSQMDISERRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKVVLRILDPSAAMLGIDALGYEPDQKEMFLEALHKPQ
GMLLITGPTGSGKTVSLYTGINILNTGATNISTAEDPVEINLEGINQVNVNAKVGLTFSNALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKTVLDVPRQSLLEVGFT
DDDLDDPNSTIYEPVGCSECREGYKGRVGIYEVMKVSPDISRIIMEDGNAIDIKDAAVKNGFRDLRRSGILKVLQGVTSI
QEMMRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=154987 AK823_RS00375 WP_068325362.1 84056..85765(-) (pilB) [Psychrobacter sp. P2G3]
GTGTCTACTACAACCAGTACATTCGGTGGTTTAGCACAGCAGCTAACTAATGCAGGTATCGTTAGCGAAGCGCACATGAA
AACAGCACAGACTGAGTCGCAACAACAGCAAATCAGTCTCGTTTCGTATTTAGTAGACCATAATATGGCCAATGCCTATC
AACTTGCCCTGTTGATGTCGCAATCATTTGGCGATCCCTTTTTCGATCTGAGCGTGCTTAGTAACGATGTCATCCCAAAA
GATTTAGTTGATGAAAAGATTGTCCGTAAATTTAATGCCCTGCCTATCTTTAAGCGCGGCCAGCGAGTATTTGTTGCTTT
AAGTGATCCTACCCGTATTGATGCGATTGATGCGATTGCTTTTAATTCGCGTCTATCTGTTGAAACGGTGGTGGTCGAAG
AAGACAAGCTCAAAAAACGTATTGATAGTGTCTATGCAGATACCATGCAGAGTTTTGGTAACTTCGCTGATGGCGACTTA
AATGTTGGCTTTGAAGACACTGAAGAGGATGACAGCGAGACCACTCTTACCGACAGTATTGATGAGGCACCCGTTGTAAA
GTTCGTTAATAAGATGCTAGTTGACGCTATTCGTATGGGCGCATCTGACTTACATTTTGAGCCTTACGAGAAGTCTTATC
GCGTGCGCTTTCGGGTCGACGGGGTAATGGAGAAAATGGCAAATCCGCCAGTGCAATTAGCAGGGAAAATCGCAGCCCGT
CTTAAAGTCATGTCACAAATGGATATCTCAGAGCGCCGCGTACCACAAGATGGCCGTATTAAGCTTAAGTTATCTAAAAA
TAAGGCTATTGACTTTCGAGTGAACTCTTTGCCAACGCTATTCGGTGAAAAGGTCGTGCTGCGTATTTTAGATCCCTCTG
CGGCGATGCTTGGTATCGACGCTTTGGGTTATGAGCCTGATCAAAAAGAGATGTTTTTGGAAGCGCTTCACAAACCGCAG
GGCATGTTATTAATTACTGGTCCTACAGGCTCTGGTAAAACGGTATCGTTATATACAGGTATCAACATTTTGAACACTGG
AGCGACCAATATATCTACAGCAGAAGATCCTGTTGAAATTAACCTTGAGGGGATCAATCAGGTCAACGTCAACGCTAAAG
TAGGTCTGACCTTTTCCAATGCTTTAAAATCGTTTTTGCGTCAAGATCCAGACATCGTCATGGTTGGTGAGATTCGTGAT
CTTGAGACCGCTGAGATTGCCATTAAGGCAGCACAAACCGGCCATATGGTACTGTCAACTCTCCATACTAACTCTGCTCC
GGAAACTTTGACTCGATTACGGAATATGGGCGTAGCATCTTTTAATATTGCTACCTCAGTCAACTTAGTTATTGCTCAGC
GACTAGCACGACGTTTATGCAAAAATTGCAAAACTGTGCTTGATGTACCGAGGCAGAGCTTACTTGAGGTTGGCTTTACC
GATGATGATCTCGACGATCCAAATAGTACCATCTATGAGCCAGTCGGTTGTAGCGAGTGCCGTGAAGGCTACAAAGGTCG
TGTGGGTATTTATGAGGTTATGAAAGTCAGCCCAGATATTTCACGCATTATTATGGAAGACGGTAATGCGATCGATATCA
AAGATGCTGCTGTCAAAAATGGCTTTCGTGATTTGCGACGCTCAGGCATTTTAAAAGTACTGCAAGGTGTCACTAGTATT
CAAGAAATGATGCGAGTAACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.606

99.824

0.675
  pilB Acinetobacter baylyi ADP1

64.085

99.824

0.64
  pilB Legionella pneumophila strain ERS1305867

54.089

94.552

0.511
  pilF Neisseria gonorrhoeae MS11

51.521

98.243

0.506
  pilB Vibrio cholerae strain A1552

48.936

99.121

0.485
  pilB Vibrio campbellii strain DS40M4

46.986

99.121

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

49.438

93.849

0.464

Multiple sequence alignment