Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KS2013_RS08795 Genome accession   NZ_CP012418
Coordinates   1901042..1902754 (-) Length   570 a.a.
NCBI ID   WP_068992651.1    Uniprot ID   A0A1B3BCA9
Organism   Kangiella sediminilitoris strain KCTC 23892     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1896042..1907754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KS2013_RS08770 (KS2013_1737) nadC 1896419..1897288 (+) 870 WP_068992635.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KS2013_RS08775 (KS2013_1738) zapD 1897332..1898099 (-) 768 WP_068992638.1 cell division protein ZapD -
  KS2013_RS08780 (KS2013_1739) coaE 1898105..1898725 (-) 621 WP_068992641.1 dephospho-CoA kinase -
  KS2013_RS08785 (KS2013_1740) - 1898738..1899646 (-) 909 WP_068992644.1 A24 family peptidase -
  KS2013_RS08790 (KS2013_1741) pilC 1899671..1900915 (-) 1245 WP_068992648.1 type II secretion system F family protein Machinery gene
  KS2013_RS08795 (KS2013_1742) pilB 1901042..1902754 (-) 1713 WP_068992651.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KS2013_RS08800 (KS2013_1743) - 1902822..1904543 (-) 1722 WP_169816866.1 PglL family O-oligosaccharyltransferase -
  KS2013_RS08805 (KS2013_1744) - 1904603..1905043 (-) 441 WP_068992656.1 pilin -
  KS2013_RS08810 (KS2013_1745) yacG 1905561..1905758 (+) 198 WP_068992659.1 DNA gyrase inhibitor YacG -
  KS2013_RS08815 (KS2013_1746) mutT 1905755..1906138 (-) 384 WP_068992662.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62748.02 Da        Isoelectric Point: 5.0099

>NTDB_id=154360 KS2013_RS08795 WP_068992651.1 1901042..1902754(-) (pilB) [Kangiella sediminilitoris strain KCTC 23892]
MALVETGLTGLAHMLVAEGLIEKDKVREAIERAKEEKVGLIDWLVNAGWVEGRSAVKAQGVNFGAPFFDVSALNVEDLPK
DIIDLNMMRKHRAIPLFKRGSRLFIGVSDATNLKSLEEIRFQTNCSIEAVLVEPSRLDTLVDKLIEEKENESLSDFDDAD
LDNIDLDAVDDSEASDVDEGAEKDDAPVVKFVKKMLVDAIRKGASDLHFEPYEKKYRVRFRIDGMLHEVASPPIQLSARI
SARLKVLSNLDISERRVPQDGRVKLKLSRTKAIDFRVNTLPTLFGEKIVMRILDPSSAMLGVDALGFEPKQKEHFMTAIN
KPQGMVLVTGPTGSGKTVTLYTGVNILNDEHKNISTAEDPVEINLAGVNQVNVNIKAGLTFASALRAFLRQDPDIVLVGE
IRDLETAEIAIKASQTGHLVLSTLHTNSAPETLTRLVNMGVAPFNIATTVNLVVAQRLARRLCEHCKKPSELPEEALLEE
GFTKEELNELTVYEPVGCDKCTLGYKGRVGLFQVMPVSEATGRIIMEGGNAIDIADQAKKENIPDLRRAGLNKIKLGATS
LEEVNRVTQD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=154360 KS2013_RS08795 WP_068992651.1 1901042..1902754(-) (pilB) [Kangiella sediminilitoris strain KCTC 23892]
ATGGCGTTAGTTGAAACAGGTTTGACGGGCTTAGCCCACATGCTGGTAGCAGAAGGCCTTATTGAAAAAGATAAAGTTAG
AGAGGCAATTGAGCGAGCCAAAGAAGAGAAGGTTGGGCTTATTGACTGGCTGGTTAATGCTGGCTGGGTGGAAGGAAGAA
GTGCCGTAAAGGCACAGGGAGTTAATTTCGGAGCTCCTTTTTTTGATGTCTCTGCATTAAACGTCGAAGACTTACCAAAA
GATATCATTGACCTGAACATGATGCGAAAGCATCGTGCTATCCCTTTATTTAAACGTGGCAGTCGCTTGTTCATTGGTGT
TTCCGATGCGACTAACCTTAAAAGTTTGGAAGAAATTCGATTCCAGACGAACTGCTCGATTGAGGCTGTACTCGTAGAGC
CTTCGAGATTAGACACACTTGTAGATAAGTTGATTGAAGAGAAAGAGAATGAGTCTCTGTCTGATTTTGATGACGCAGAC
CTTGATAACATTGACCTTGATGCGGTTGATGATAGTGAAGCTTCCGATGTAGATGAAGGAGCTGAAAAAGATGATGCTCC
AGTAGTGAAGTTTGTTAAGAAAATGCTGGTTGATGCTATCCGTAAAGGGGCATCCGATTTGCACTTCGAGCCATATGAAA
AGAAGTATCGTGTACGTTTCCGTATTGATGGTATGTTACACGAAGTTGCAAGCCCTCCTATTCAGTTATCAGCACGTATT
TCTGCACGCTTGAAGGTTTTATCAAATTTGGATATCTCTGAAAGGCGGGTTCCTCAGGATGGCCGTGTAAAATTAAAGCT
TTCAAGAACCAAAGCTATTGATTTTCGTGTGAATACTTTGCCGACTCTATTTGGTGAGAAGATTGTAATGCGTATTCTTG
ATCCCTCCAGTGCAATGTTAGGAGTGGATGCGTTAGGTTTTGAACCTAAGCAAAAAGAGCACTTTATGACTGCTATCAAT
AAACCTCAGGGAATGGTTTTGGTTACGGGGCCTACAGGTTCGGGTAAAACGGTAACCCTATATACTGGCGTGAATATTTT
AAATGATGAGCATAAAAATATTTCGACAGCTGAGGACCCGGTAGAGATTAACTTGGCTGGTGTTAACCAGGTCAACGTTA
ACATAAAAGCTGGATTAACCTTTGCTTCGGCTCTGAGAGCATTTCTACGTCAGGATCCGGATATTGTACTGGTGGGTGAG
ATCCGAGACCTTGAGACAGCTGAAATTGCTATTAAGGCATCTCAGACGGGTCACTTAGTACTTTCCACACTACATACAAA
CTCTGCACCTGAGACATTAACTCGTTTGGTTAATATGGGCGTAGCGCCTTTTAATATCGCAACCACTGTTAACTTGGTTG
TTGCACAGCGATTAGCCAGAAGACTGTGTGAGCATTGTAAGAAACCTTCTGAATTACCAGAAGAAGCGTTACTGGAAGAG
GGCTTCACTAAAGAAGAGCTGAACGAACTCACAGTTTACGAACCTGTAGGTTGCGATAAGTGTACATTAGGTTATAAAGG
TCGTGTAGGTTTATTCCAGGTAATGCCAGTATCCGAGGCAACTGGAAGAATAATTATGGAAGGTGGAAACGCGATTGATA
TCGCAGATCAGGCCAAAAAAGAGAATATTCCTGATTTACGGAGAGCTGGGTTAAACAAAATTAAATTAGGGGCTACCAGT
CTGGAAGAAGTTAATCGAGTGACGCAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B3BCA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.106

99.123

0.556
  pilB Acinetobacter baumannii D1279779

55.83

99.298

0.554
  pilB Legionella pneumophila strain ERS1305867

53.136

100

0.535
  pilF Neisseria gonorrhoeae MS11

51.456

90.351

0.465
  pilB Vibrio cholerae strain A1552

50.685

89.649

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

45.811

98.421

0.451
  pilB Vibrio campbellii strain DS40M4

45.276

98.421

0.446
  pilF Thermus thermophilus HB27

38.146

98.421

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.966

100

0.375

Multiple sequence alignment