Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FORC36_RS00535 Genome accession   NZ_CP015512
Coordinates   119547..121046 (+) Length   499 a.a.
NCBI ID   WP_011078943.1    Uniprot ID   A0A3Q0L2V2
Organism   Vibrio vulnificus strain FORC_036     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114547..126046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC36_RS00515 (FORC36_0089) hslO 114936..115811 (-) 876 WP_011078947.1 Hsp33 family molecular chaperone HslO -
  FORC36_RS00520 (FORC36_0090) hslR 115843..116229 (-) 387 WP_085760632.1 ribosome-associated heat shock protein Hsp15 -
  FORC36_RS00525 (FORC36_0092) gspC 116614..117489 (+) 876 WP_157401904.1 type II secretion system protein GspC -
  FORC36_RS00530 (FORC36_0093) gspD 117526..119547 (+) 2022 WP_011078944.1 type II secretion system secretin GspD -
  FORC36_RS00535 (FORC36_0094) pilB/pilB1 119547..121046 (+) 1500 WP_011078943.1 type II secretion system ATPase GspE Machinery gene
  FORC36_RS00540 (FORC36_0095) gspF 121046..122263 (+) 1218 WP_011078942.1 type II secretion system inner membrane protein GspF -
  FORC36_RS00545 (FORC36_0096) gspG 122317..122760 (+) 444 WP_011078941.1 type II secretion system major pseudopilin GspG -
  FORC36_RS00550 (FORC36_0097) gspH 122687..123391 (+) 705 WP_085760633.1 type II secretion system minor pseudopilin GspH -
  FORC36_RS00555 (FORC36_0098) gspI 123384..123746 (+) 363 WP_085760634.1 type II secretion system minor pseudopilin GspI -
  FORC36_RS00560 (FORC36_0099) gspJ 123733..124434 (+) 702 WP_065090793.1 type II secretion system minor pseudopilin GspJ -
  FORC36_RS00565 (FORC36_0100) gspK 124427..125449 (+) 1023 WP_085760635.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55846.14 Da        Isoelectric Point: 6.0826

>NTDB_id=153987 FORC36_RS00535 WP_011078943.1 119547..121046(+) (pilB/pilB1) [Vibrio vulnificus strain FORC_036]
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=153987 FORC36_RS00535 WP_011078943.1 119547..121046(+) (pilB/pilB1) [Vibrio vulnificus strain FORC_036]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AGTCGAGCATCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACGTTTGTCCCTCAAGCCATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGCCAGTTGATGGAAGACATTGGCTCGGATGATGACTTCTTCTCTTTGGCTGAAGAGTTGCCGCAAGA
TGAAGATTTGCTCGAAACGGAAGACGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GGGCGTCAGATATTCACATCGAAACGTTCGAGAAAATTCTGTCGATTCGTTTCCGCGTCGATGGCGTTTTGCGCGATGTA
CTGTCTCCGAGTCGCAAACTGGCGCCGCTGTTGGTGTCACGTGTTAAAGTTATGGCCAAACTGGATATTGCAGAAAAACG
TGTGCCACAAGATGGCCGTATTTCACTGCGTATCGGTGGTCGCGCTGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGCGAGCGTGTGGTGATGCGTTTGCTGGATAAAAACGCCACTCGTCTTGACCTGCATAGCCTAGGCATGACACCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGTCCTCACGGCATTATCTTGGTCACAGGTCCAACGGGTTCGGGTAAATC
CACCACCTTGTACGCTGGCCTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGACCCGATCGAATTTG
ATATTGATGGTATCGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCTCGTGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAAATTCGTGACTTGGAAACCGCGCAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCCGTAGGTGCAATTACCCGTCTGCGTGACATGGGCATTGAGCCCT
TTTTGATCTCTTCTTCACTGCTGGGCGTGCTGGCTCAGCGCCTGGTACGGACTTTATGCCAAGACTGTAAAGAACCGTAC
GAGGCGGACAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACTTTATATCGCGCCAAGGGATGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACCGGTATCCATGAGCTGTTGATGGTGGACGATAAAGTTCAAGAGCTGA
TCCATGCTGAAGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCGAGCATTCGCAGCGACGGTTTAGAC
AAAGTGTTGCAAGGCGTGACGTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L2V2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.966

100

0.453
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.713

98.998

0.423
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363

Multiple sequence alignment